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Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs.

Petrini S, Ramadori G, Villa R, Borghetti P, de Angelis E, Cantoni AM, Corradi A, Amici A, Ferrari M - Vaccines (Basel) (2013)

Bottom Line: Only vaccines A and B were able to reduce the clinical signs of the infection.Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups.In the other groups, the IFN-g were detected after challenge infection.

View Article: PubMed Central - PubMed

Affiliation: Umbria and Marche Experimental Zooprophylaxis Institute, via Gaetano Salvemini 1, Perugia 06126, Italy. s.petrini@izsum.it.

ABSTRACT
In veterinary medicine, there have been different experiences with the plasmid DNA vaccination. In this area and with the hypothesis to demonstrate the effectiveness of different plasmids encoding porcine respiratory and reproductive syndrome (PRRS), five DNA vaccines against PRRS were evaluated for their innocuity and efficacy in pigs. Eighteen animals were divided into five groups which were injected with five (A, B, C, D, E) different DNA vaccines. Albeit, none of the proposed vaccines were able to protect the animals against PRRS virus. Only vaccines A and B were able to reduce the clinical signs of the infection. ELISA IgM were detected 30 days after the first vaccination in the pigs injected by Vaccine A or B. ELISA IgG were detected 90 days after the first vaccination in the pigs injected by Vaccine B or C. Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups. In the pigs inoculated with Vaccine C, IFN-g were detected 90 days after first vaccination, and after challenge exposure they increased. In the other groups, the IFN-g were detected after challenge infection. Pigs injected with each of the vaccines A, B, C, D and E showed a significantly higher level of CD4(-)CD8⁺ lymphocytes (p < 0.001) after infection in comparison with their controls.

No MeSH data available.


Related in: MedlinePlus

IFN-γ response (pg/mL) in pigs immunised with experimental PRRS DNA vaccines and challenge infected with virulent PRRSV.
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vaccines-01-00463-f008: IFN-γ response (pg/mL) in pigs immunised with experimental PRRS DNA vaccines and challenge infected with virulent PRRSV.

Mentions: IFN-γ were detected only in the groups of pigs inoculated with vaccines B or C on PVD 84 with a mean titre of 13 and 10 pg/mL, respectively. A further increase was detected in these animals on PCD 14 when the mean was 41 and 59 pg/mL, respectively. In the other groups immunized with Vaccines A, D, E, plasmid vector and in the challenge infection controls, IFN-γ were detected only on PCD 14, with a mean titre ranging from 12–36 pg/mL. In all groups, a decreased of IFN-γ was detected on PCD 61 with a mean titres of 2 pg/mL (Figure 8).


Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs.

Petrini S, Ramadori G, Villa R, Borghetti P, de Angelis E, Cantoni AM, Corradi A, Amici A, Ferrari M - Vaccines (Basel) (2013)

IFN-γ response (pg/mL) in pigs immunised with experimental PRRS DNA vaccines and challenge infected with virulent PRRSV.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494207&req=5

vaccines-01-00463-f008: IFN-γ response (pg/mL) in pigs immunised with experimental PRRS DNA vaccines and challenge infected with virulent PRRSV.
Mentions: IFN-γ were detected only in the groups of pigs inoculated with vaccines B or C on PVD 84 with a mean titre of 13 and 10 pg/mL, respectively. A further increase was detected in these animals on PCD 14 when the mean was 41 and 59 pg/mL, respectively. In the other groups immunized with Vaccines A, D, E, plasmid vector and in the challenge infection controls, IFN-γ were detected only on PCD 14, with a mean titre ranging from 12–36 pg/mL. In all groups, a decreased of IFN-γ was detected on PCD 61 with a mean titres of 2 pg/mL (Figure 8).

Bottom Line: Only vaccines A and B were able to reduce the clinical signs of the infection.Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups.In the other groups, the IFN-g were detected after challenge infection.

View Article: PubMed Central - PubMed

Affiliation: Umbria and Marche Experimental Zooprophylaxis Institute, via Gaetano Salvemini 1, Perugia 06126, Italy. s.petrini@izsum.it.

ABSTRACT
In veterinary medicine, there have been different experiences with the plasmid DNA vaccination. In this area and with the hypothesis to demonstrate the effectiveness of different plasmids encoding porcine respiratory and reproductive syndrome (PRRS), five DNA vaccines against PRRS were evaluated for their innocuity and efficacy in pigs. Eighteen animals were divided into five groups which were injected with five (A, B, C, D, E) different DNA vaccines. Albeit, none of the proposed vaccines were able to protect the animals against PRRS virus. Only vaccines A and B were able to reduce the clinical signs of the infection. ELISA IgM were detected 30 days after the first vaccination in the pigs injected by Vaccine A or B. ELISA IgG were detected 90 days after the first vaccination in the pigs injected by Vaccine B or C. Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups. In the pigs inoculated with Vaccine C, IFN-g were detected 90 days after first vaccination, and after challenge exposure they increased. In the other groups, the IFN-g were detected after challenge infection. Pigs injected with each of the vaccines A, B, C, D and E showed a significantly higher level of CD4(-)CD8⁺ lymphocytes (p < 0.001) after infection in comparison with their controls.

No MeSH data available.


Related in: MedlinePlus