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Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs.

Petrini S, Ramadori G, Villa R, Borghetti P, de Angelis E, Cantoni AM, Corradi A, Amici A, Ferrari M - Vaccines (Basel) (2013)

Bottom Line: Only vaccines A and B were able to reduce the clinical signs of the infection.Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups.In the other groups, the IFN-g were detected after challenge infection.

View Article: PubMed Central - PubMed

Affiliation: Umbria and Marche Experimental Zooprophylaxis Institute, via Gaetano Salvemini 1, Perugia 06126, Italy. s.petrini@izsum.it.

ABSTRACT
In veterinary medicine, there have been different experiences with the plasmid DNA vaccination. In this area and with the hypothesis to demonstrate the effectiveness of different plasmids encoding porcine respiratory and reproductive syndrome (PRRS), five DNA vaccines against PRRS were evaluated for their innocuity and efficacy in pigs. Eighteen animals were divided into five groups which were injected with five (A, B, C, D, E) different DNA vaccines. Albeit, none of the proposed vaccines were able to protect the animals against PRRS virus. Only vaccines A and B were able to reduce the clinical signs of the infection. ELISA IgM were detected 30 days after the first vaccination in the pigs injected by Vaccine A or B. ELISA IgG were detected 90 days after the first vaccination in the pigs injected by Vaccine B or C. Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups. In the pigs inoculated with Vaccine C, IFN-g were detected 90 days after first vaccination, and after challenge exposure they increased. In the other groups, the IFN-g were detected after challenge infection. Pigs injected with each of the vaccines A, B, C, D and E showed a significantly higher level of CD4(-)CD8⁺ lymphocytes (p < 0.001) after infection in comparison with their controls.

No MeSH data available.


Related in: MedlinePlus

Plasmid pVAX1-48CpG.
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vaccines-01-00463-f006: Plasmid pVAX1-48CpG.

Mentions: Plasmid pVAX1-48CpG (Figure 6) was constructed by introduction in pVAX1 of specific CpG motifs based on the immunostimulatory sequence from ODN 2135 [13]. Briefly, two complementary oligodeoxynucleotides (BstE II forw: 5'-AATTCGGTTACCTCTAGACAAACCAACCAAT-3'; BstE II rew: 5'-CTAGATTGGTTGGTTGGTCTAGAGGTAACCG-3') were annealed to form a duplex containing the restriction site BstE II, and then cloned between EcoR I and Xba I sites in pCDNA3.1 (Invitrogen, San Diego, CA, USA). Another two complementary oligodeoxynucleotides were annealed to form a duplex containing 12 CpG motifs with protruding ends complementary to the restriction site BstE II.


Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs.

Petrini S, Ramadori G, Villa R, Borghetti P, de Angelis E, Cantoni AM, Corradi A, Amici A, Ferrari M - Vaccines (Basel) (2013)

Plasmid pVAX1-48CpG.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494207&req=5

vaccines-01-00463-f006: Plasmid pVAX1-48CpG.
Mentions: Plasmid pVAX1-48CpG (Figure 6) was constructed by introduction in pVAX1 of specific CpG motifs based on the immunostimulatory sequence from ODN 2135 [13]. Briefly, two complementary oligodeoxynucleotides (BstE II forw: 5'-AATTCGGTTACCTCTAGACAAACCAACCAAT-3'; BstE II rew: 5'-CTAGATTGGTTGGTTGGTCTAGAGGTAACCG-3') were annealed to form a duplex containing the restriction site BstE II, and then cloned between EcoR I and Xba I sites in pCDNA3.1 (Invitrogen, San Diego, CA, USA). Another two complementary oligodeoxynucleotides were annealed to form a duplex containing 12 CpG motifs with protruding ends complementary to the restriction site BstE II.

Bottom Line: Only vaccines A and B were able to reduce the clinical signs of the infection.Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups.In the other groups, the IFN-g were detected after challenge infection.

View Article: PubMed Central - PubMed

Affiliation: Umbria and Marche Experimental Zooprophylaxis Institute, via Gaetano Salvemini 1, Perugia 06126, Italy. s.petrini@izsum.it.

ABSTRACT
In veterinary medicine, there have been different experiences with the plasmid DNA vaccination. In this area and with the hypothesis to demonstrate the effectiveness of different plasmids encoding porcine respiratory and reproductive syndrome (PRRS), five DNA vaccines against PRRS were evaluated for their innocuity and efficacy in pigs. Eighteen animals were divided into five groups which were injected with five (A, B, C, D, E) different DNA vaccines. Albeit, none of the proposed vaccines were able to protect the animals against PRRS virus. Only vaccines A and B were able to reduce the clinical signs of the infection. ELISA IgM were detected 30 days after the first vaccination in the pigs injected by Vaccine A or B. ELISA IgG were detected 90 days after the first vaccination in the pigs injected by Vaccine B or C. Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups. In the pigs inoculated with Vaccine C, IFN-g were detected 90 days after first vaccination, and after challenge exposure they increased. In the other groups, the IFN-g were detected after challenge infection. Pigs injected with each of the vaccines A, B, C, D and E showed a significantly higher level of CD4(-)CD8⁺ lymphocytes (p < 0.001) after infection in comparison with their controls.

No MeSH data available.


Related in: MedlinePlus