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Pilot Study on the Use of DNA Priming Immunization to Enhance Y. pestis LcrV-Specific B Cell Responses Elicited by a Recombinant LcrV Protein Vaccine.

Li W, Wang S, Lu S - Vaccines (Basel) (2013)

Bottom Line: However, there is limited information on the mechanism of this effect.Previously, we demonstrated that a DNA vaccine expressing LcrV antigen can protect mice from lethal mucosal challenge.The finding that DNA immunization can enhance antigen-specific B cell responses is highly significant and will help guide similar studies in other model antigen systems.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA. wei.li@umassmed.edu.

ABSTRACT
Recent studies indicate that DNA immunization is powerful in eliciting antigen-specific antibody responses in both animal and human studies. However, there is limited information on the mechanism of this effect. In particular, it is not known whether DNA immunization can also enhance the development of antigen-specific B cell development. In this report, a pilot study was conducted using plague LcrV immunogen as a model system to determine whether DNA immunization is able to enhance LcrV-specific B cell development in mice. Plague is an acute and often fatal infectious disease caused by Yersinia pestis (Y. pestis). Humoral immune responses provide critical protective immunity against plague. Previously, we demonstrated that a DNA vaccine expressing LcrV antigen can protect mice from lethal mucosal challenge. In the current study, we further evaluated whether the use of a DNA priming immunization is able to enhance the immunogenicity of a recombinant LcrV protein vaccine, and in particular, the development of LcrV-specific B cells. Our data indicate that DNA immunization was able to elicit high-level LcrV antibody responses when used alone or as part of a prime-boost immunization approach. Most significantly, DNA immunization was also able to increase the levels of LcrV-specific B cell development. The finding that DNA immunization can enhance antigen-specific B cell responses is highly significant and will help guide similar studies in other model antigen systems.

No MeSH data available.


Related in: MedlinePlus

V-specific temporal antibody responses in mice immunized with different vaccination regimens: codon optimized V DNA vaccine alone (V-DNA), V DNA vaccine prime followed by V protein boost formulated with IFA (V-DNA+Prot/IFA), V protein formulated with IFA (V-Prot/IFA), V protein alone (V-Prot), or empty DNA vaccine vector alone (Vector). V-specific antibody responses were measured by ELISA at different time points using pooled mouse sera from each group against V protein. Each curve represents mean OD values with standard error of duplicated assays for each mouse group, at 1:500 serum dilution.
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vaccines-02-00036-f002: V-specific temporal antibody responses in mice immunized with different vaccination regimens: codon optimized V DNA vaccine alone (V-DNA), V DNA vaccine prime followed by V protein boost formulated with IFA (V-DNA+Prot/IFA), V protein formulated with IFA (V-Prot/IFA), V protein alone (V-Prot), or empty DNA vaccine vector alone (Vector). V-specific antibody responses were measured by ELISA at different time points using pooled mouse sera from each group against V protein. Each curve represents mean OD values with standard error of duplicated assays for each mouse group, at 1:500 serum dilution.

Mentions: Gene gun delivery of the LcrV DNA vaccine was highly effective and positive antibody responses in the DNA Alone Group were detected even after a single immunization (Figure 2). The 2nd immunization was able to further increase antibody response levels, which continued to increase for about four weeks. The temporal antibody response pattern in the Prime-Boost Group was similar. In contrast, the Protein Alone Group had delayed and lower level antibody responses after one immunization, but was able to reach the same levels as those in the first two groups after the 2nd immunization. Adjuvant is important for the immunogenicity of LcrV protein vaccines as the LcrV protein alone without IFA (Protein No Adjuvant Group) was not able to elicit the same levels of antibody responses, even after two immunizations. There was no LcrV antibody response in the Negative Control Group.


Pilot Study on the Use of DNA Priming Immunization to Enhance Y. pestis LcrV-Specific B Cell Responses Elicited by a Recombinant LcrV Protein Vaccine.

Li W, Wang S, Lu S - Vaccines (Basel) (2013)

V-specific temporal antibody responses in mice immunized with different vaccination regimens: codon optimized V DNA vaccine alone (V-DNA), V DNA vaccine prime followed by V protein boost formulated with IFA (V-DNA+Prot/IFA), V protein formulated with IFA (V-Prot/IFA), V protein alone (V-Prot), or empty DNA vaccine vector alone (Vector). V-specific antibody responses were measured by ELISA at different time points using pooled mouse sera from each group against V protein. Each curve represents mean OD values with standard error of duplicated assays for each mouse group, at 1:500 serum dilution.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494201&req=5

vaccines-02-00036-f002: V-specific temporal antibody responses in mice immunized with different vaccination regimens: codon optimized V DNA vaccine alone (V-DNA), V DNA vaccine prime followed by V protein boost formulated with IFA (V-DNA+Prot/IFA), V protein formulated with IFA (V-Prot/IFA), V protein alone (V-Prot), or empty DNA vaccine vector alone (Vector). V-specific antibody responses were measured by ELISA at different time points using pooled mouse sera from each group against V protein. Each curve represents mean OD values with standard error of duplicated assays for each mouse group, at 1:500 serum dilution.
Mentions: Gene gun delivery of the LcrV DNA vaccine was highly effective and positive antibody responses in the DNA Alone Group were detected even after a single immunization (Figure 2). The 2nd immunization was able to further increase antibody response levels, which continued to increase for about four weeks. The temporal antibody response pattern in the Prime-Boost Group was similar. In contrast, the Protein Alone Group had delayed and lower level antibody responses after one immunization, but was able to reach the same levels as those in the first two groups after the 2nd immunization. Adjuvant is important for the immunogenicity of LcrV protein vaccines as the LcrV protein alone without IFA (Protein No Adjuvant Group) was not able to elicit the same levels of antibody responses, even after two immunizations. There was no LcrV antibody response in the Negative Control Group.

Bottom Line: However, there is limited information on the mechanism of this effect.Previously, we demonstrated that a DNA vaccine expressing LcrV antigen can protect mice from lethal mucosal challenge.The finding that DNA immunization can enhance antigen-specific B cell responses is highly significant and will help guide similar studies in other model antigen systems.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA. wei.li@umassmed.edu.

ABSTRACT
Recent studies indicate that DNA immunization is powerful in eliciting antigen-specific antibody responses in both animal and human studies. However, there is limited information on the mechanism of this effect. In particular, it is not known whether DNA immunization can also enhance the development of antigen-specific B cell development. In this report, a pilot study was conducted using plague LcrV immunogen as a model system to determine whether DNA immunization is able to enhance LcrV-specific B cell development in mice. Plague is an acute and often fatal infectious disease caused by Yersinia pestis (Y. pestis). Humoral immune responses provide critical protective immunity against plague. Previously, we demonstrated that a DNA vaccine expressing LcrV antigen can protect mice from lethal mucosal challenge. In the current study, we further evaluated whether the use of a DNA priming immunization is able to enhance the immunogenicity of a recombinant LcrV protein vaccine, and in particular, the development of LcrV-specific B cells. Our data indicate that DNA immunization was able to elicit high-level LcrV antibody responses when used alone or as part of a prime-boost immunization approach. Most significantly, DNA immunization was also able to increase the levels of LcrV-specific B cell development. The finding that DNA immunization can enhance antigen-specific B cell responses is highly significant and will help guide similar studies in other model antigen systems.

No MeSH data available.


Related in: MedlinePlus