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Partial hepatectomy induces delayed hepatocyte proliferation and normal liver regeneration in ovariectomized mice.

Umeda M, Hiramoto M, Imai T - Clin Exp Gastroenterol (2015)

Bottom Line: Both E2 administration and PH induced the gene expression of estrogen receptor α (ERα).The transcripts of ERα were detected specifically in periportal hepatocytes after E2 administration and PH.Moreover, the E2 concentrations and hepatocyte proliferation rates were highest in the proestrus period of the estrous cycle.

View Article: PubMed Central - PubMed

Affiliation: Department of Aging Intervention, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan.

ABSTRACT
Estrogens play central roles in sexual development, reproduction, and hepatocyte proliferation. The ovaries are one of the main organs for estradiol (E2) production. Ovariectomies (OVXs) were performed on the female mice, and hepatocyte proliferation was analyzed. The ovariectomized mice exhibited delayed hepatocyte proliferation after partial hepatectomy (PH) and also exhibited delayed and reduced E2 induction. Both E2 administration and PH induced the gene expression of estrogen receptor α (ERα). The transcripts of ERα were detected specifically in periportal hepatocytes after E2 administration and PH. Moreover, the E2 concentrations and hepatocyte proliferation rates were highest in the proestrus period of the estrous cycle. Taken together, these findings indicate that E2 accelerated ERα expression in periportal hepatocytes and hepatocyte proliferation in the female mice.

No MeSH data available.


PH induced ERα expression in the hepatocyte of periportal area.Notes: PH-stimulated ERα expression in the periportal hepatocytes. After the PH operations, the livers were removed from these mice. ERα expression levels were analyzed with RT-PCR (A) and ISH (B). (A) The liver RNAs of the control (lanes 2, 7, and 10), PH (2-h lane 4, 8-h lanes 5, 9, and 11, 24-h lanes 6 and 12), the E2-injected mice (lanes 7, 8, and 9), and OVX (lanes 10, 11, and 12) were extracted, and RT-PCR was performed. (B) The livers were removed from the control (a), the PH (b), and the OVX-PH (c) mice. ISH was performed using ERα probes. Scale bars =1 mm. The white arrows indicated portal veins.Abbreviations: PH, partial hepatectomy; ERα, estrogen receptor α; RT-PCR, reverse transcription polymerase chain reaction; ISH, in situ hybridization; OVX, ovariectomy; E2, estradiol; HPRT, hypoxanthine-guanine phosphoribosyltransferase.
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f4-ceg-8-175: PH induced ERα expression in the hepatocyte of periportal area.Notes: PH-stimulated ERα expression in the periportal hepatocytes. After the PH operations, the livers were removed from these mice. ERα expression levels were analyzed with RT-PCR (A) and ISH (B). (A) The liver RNAs of the control (lanes 2, 7, and 10), PH (2-h lane 4, 8-h lanes 5, 9, and 11, 24-h lanes 6 and 12), the E2-injected mice (lanes 7, 8, and 9), and OVX (lanes 10, 11, and 12) were extracted, and RT-PCR was performed. (B) The livers were removed from the control (a), the PH (b), and the OVX-PH (c) mice. ISH was performed using ERα probes. Scale bars =1 mm. The white arrows indicated portal veins.Abbreviations: PH, partial hepatectomy; ERα, estrogen receptor α; RT-PCR, reverse transcription polymerase chain reaction; ISH, in situ hybridization; OVX, ovariectomy; E2, estradiol; HPRT, hypoxanthine-guanine phosphoribosyltransferase.

Mentions: The mRNA expressions of ERα and ERβ in the liver were analyzed using reverse transcription polymerase chain reaction (RT-PCR) (Figure 4A) and in situ hybridization (Figure 4B). First, ERβ mRNA was detected in the positive control of ovary, and not amplified from liver samples (Figure 4A).19 PH and E2 administration induced the transcripts of ERα (Figure 4A). Note that both E2 injection and PH induced ERα expression synergetically. Delayed induced expression of ERα was observed in OVX mice at 24 hours after PH, similar to delayed E2 induction in OVX mice (Figure 1B). These observations suggested that PH induced delayed E2 concentration and ERα expression.


Partial hepatectomy induces delayed hepatocyte proliferation and normal liver regeneration in ovariectomized mice.

Umeda M, Hiramoto M, Imai T - Clin Exp Gastroenterol (2015)

PH induced ERα expression in the hepatocyte of periportal area.Notes: PH-stimulated ERα expression in the periportal hepatocytes. After the PH operations, the livers were removed from these mice. ERα expression levels were analyzed with RT-PCR (A) and ISH (B). (A) The liver RNAs of the control (lanes 2, 7, and 10), PH (2-h lane 4, 8-h lanes 5, 9, and 11, 24-h lanes 6 and 12), the E2-injected mice (lanes 7, 8, and 9), and OVX (lanes 10, 11, and 12) were extracted, and RT-PCR was performed. (B) The livers were removed from the control (a), the PH (b), and the OVX-PH (c) mice. ISH was performed using ERα probes. Scale bars =1 mm. The white arrows indicated portal veins.Abbreviations: PH, partial hepatectomy; ERα, estrogen receptor α; RT-PCR, reverse transcription polymerase chain reaction; ISH, in situ hybridization; OVX, ovariectomy; E2, estradiol; HPRT, hypoxanthine-guanine phosphoribosyltransferase.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494181&req=5

f4-ceg-8-175: PH induced ERα expression in the hepatocyte of periportal area.Notes: PH-stimulated ERα expression in the periportal hepatocytes. After the PH operations, the livers were removed from these mice. ERα expression levels were analyzed with RT-PCR (A) and ISH (B). (A) The liver RNAs of the control (lanes 2, 7, and 10), PH (2-h lane 4, 8-h lanes 5, 9, and 11, 24-h lanes 6 and 12), the E2-injected mice (lanes 7, 8, and 9), and OVX (lanes 10, 11, and 12) were extracted, and RT-PCR was performed. (B) The livers were removed from the control (a), the PH (b), and the OVX-PH (c) mice. ISH was performed using ERα probes. Scale bars =1 mm. The white arrows indicated portal veins.Abbreviations: PH, partial hepatectomy; ERα, estrogen receptor α; RT-PCR, reverse transcription polymerase chain reaction; ISH, in situ hybridization; OVX, ovariectomy; E2, estradiol; HPRT, hypoxanthine-guanine phosphoribosyltransferase.
Mentions: The mRNA expressions of ERα and ERβ in the liver were analyzed using reverse transcription polymerase chain reaction (RT-PCR) (Figure 4A) and in situ hybridization (Figure 4B). First, ERβ mRNA was detected in the positive control of ovary, and not amplified from liver samples (Figure 4A).19 PH and E2 administration induced the transcripts of ERα (Figure 4A). Note that both E2 injection and PH induced ERα expression synergetically. Delayed induced expression of ERα was observed in OVX mice at 24 hours after PH, similar to delayed E2 induction in OVX mice (Figure 1B). These observations suggested that PH induced delayed E2 concentration and ERα expression.

Bottom Line: Both E2 administration and PH induced the gene expression of estrogen receptor α (ERα).The transcripts of ERα were detected specifically in periportal hepatocytes after E2 administration and PH.Moreover, the E2 concentrations and hepatocyte proliferation rates were highest in the proestrus period of the estrous cycle.

View Article: PubMed Central - PubMed

Affiliation: Department of Aging Intervention, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan.

ABSTRACT
Estrogens play central roles in sexual development, reproduction, and hepatocyte proliferation. The ovaries are one of the main organs for estradiol (E2) production. Ovariectomies (OVXs) were performed on the female mice, and hepatocyte proliferation was analyzed. The ovariectomized mice exhibited delayed hepatocyte proliferation after partial hepatectomy (PH) and also exhibited delayed and reduced E2 induction. Both E2 administration and PH induced the gene expression of estrogen receptor α (ERα). The transcripts of ERα were detected specifically in periportal hepatocytes after E2 administration and PH. Moreover, the E2 concentrations and hepatocyte proliferation rates were highest in the proestrus period of the estrous cycle. Taken together, these findings indicate that E2 accelerated ERα expression in periportal hepatocytes and hepatocyte proliferation in the female mice.

No MeSH data available.