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Relations of Wolbachia Infection with Phylogeography of Philaenus spumarius (Hemiptera: Aphrophoridae) Populations Within and Beyond the Carpathian Contact Zone.

Lis A, Maryańska-Nadachowska A, Kajtoch Ł - Microb. Ecol. (2015)

Bottom Line: Given the reproductive alterations which are often associated with this endosymbiont, Wolbachia probably maintain genetic differentiation of its hosts in its contact zone in the Carpathians.This is one of the first studies demonstrating the presence of Wolbachia across a large part of the range of insect species, including the contact zone.The spread of Wolbachia in P. spumarius populations can potentially cause speciation by compromising the potential reproductive barrier between infected and uninfected populations.

View Article: PubMed Central - PubMed

Affiliation: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska St. 17, 31-016, Cracow, Poland, lis@isez.pan.krakow.pl.

ABSTRACT
Wolbachia is the most widespread intracellular α-proteobacteria maternally inherited endosymbiont of insects and nematodes. These bacteria are associated with a number of different reproductive phenotypes of their hosts. Relatively few studies have dealt with distribution of infections across populations and with the influence of these bacteria on host genetic diversification and speciation. The aims of this study are to determine the distribution and rate of infection and to characterize the Wolbachia strains associated with Philaenus spumarius spittlebug (Hemiptera) by using multilocus sequencing typing (MLST) analysis and host phylogeography. The results showed that infection rate was significantly different between members of both main mitochondrial phylogenetic lineages of P. spumarius. We detected much higher infection rates of Wolbachia in P. spumarius populations from the north-east clade than the south-west clade. Moreover, the frequency of these infections varied within and outside the contact zone known from the Carpathians. Given the reproductive alterations which are often associated with this endosymbiont, Wolbachia probably maintain genetic differentiation of its hosts in its contact zone in the Carpathians. This is one of the first studies demonstrating the presence of Wolbachia across a large part of the range of insect species, including the contact zone. The spread of Wolbachia in P. spumarius populations can potentially cause speciation by compromising the potential reproductive barrier between infected and uninfected populations. We discuss possible implications of Wolbachia infection inducing cytoplasmic incompatibility in the population dynamics of this spittlebug but confirm that more studies are also required.

No MeSH data available.


Related in: MedlinePlus

Haplotype cytochrome B networks of P. spumarius and association of Wolbachia infections with haplotypes belonging to the NE and SW mitochondrial clades from the species range, including contact zone in the Carpathians (symbols of populations like in Table 1). White circles, not infected; gray circles, all population infected; black circle, some individuals infected of population
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Fig4: Haplotype cytochrome B networks of P. spumarius and association of Wolbachia infections with haplotypes belonging to the NE and SW mitochondrial clades from the species range, including contact zone in the Carpathians (symbols of populations like in Table 1). White circles, not infected; gray circles, all population infected; black circle, some individuals infected of population

Mentions: Moreover, the networks in Fig. 4 demonstrated results of relations within the P. spumarius mtDNA clades and infections of Wolbachia bacteria. This figure clearly shows that infections dominate in northeastern clade, and only in population from Norway (belonging to this clade) we did not find this microorganism. In the southwestern clade, the mtDNA network the most infected populations were from the Carpathian Mountains where the contact zone of the main phylogenetic P. spumarius clades occurs. This is where specimens can mix with the northeastern clade. Another single exception of the infected populations belonging to the southwestern clade is from Portugal and the USA. About one fifth of the infected individuals was in the population from Portugal; in the USA, one-third of the examined individuals harbored the Wolbachia.Fig. 4


Relations of Wolbachia Infection with Phylogeography of Philaenus spumarius (Hemiptera: Aphrophoridae) Populations Within and Beyond the Carpathian Contact Zone.

Lis A, Maryańska-Nadachowska A, Kajtoch Ł - Microb. Ecol. (2015)

Haplotype cytochrome B networks of P. spumarius and association of Wolbachia infections with haplotypes belonging to the NE and SW mitochondrial clades from the species range, including contact zone in the Carpathians (symbols of populations like in Table 1). White circles, not infected; gray circles, all population infected; black circle, some individuals infected of population
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4494152&req=5

Fig4: Haplotype cytochrome B networks of P. spumarius and association of Wolbachia infections with haplotypes belonging to the NE and SW mitochondrial clades from the species range, including contact zone in the Carpathians (symbols of populations like in Table 1). White circles, not infected; gray circles, all population infected; black circle, some individuals infected of population
Mentions: Moreover, the networks in Fig. 4 demonstrated results of relations within the P. spumarius mtDNA clades and infections of Wolbachia bacteria. This figure clearly shows that infections dominate in northeastern clade, and only in population from Norway (belonging to this clade) we did not find this microorganism. In the southwestern clade, the mtDNA network the most infected populations were from the Carpathian Mountains where the contact zone of the main phylogenetic P. spumarius clades occurs. This is where specimens can mix with the northeastern clade. Another single exception of the infected populations belonging to the southwestern clade is from Portugal and the USA. About one fifth of the infected individuals was in the population from Portugal; in the USA, one-third of the examined individuals harbored the Wolbachia.Fig. 4

Bottom Line: Given the reproductive alterations which are often associated with this endosymbiont, Wolbachia probably maintain genetic differentiation of its hosts in its contact zone in the Carpathians.This is one of the first studies demonstrating the presence of Wolbachia across a large part of the range of insect species, including the contact zone.The spread of Wolbachia in P. spumarius populations can potentially cause speciation by compromising the potential reproductive barrier between infected and uninfected populations.

View Article: PubMed Central - PubMed

Affiliation: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska St. 17, 31-016, Cracow, Poland, lis@isez.pan.krakow.pl.

ABSTRACT
Wolbachia is the most widespread intracellular α-proteobacteria maternally inherited endosymbiont of insects and nematodes. These bacteria are associated with a number of different reproductive phenotypes of their hosts. Relatively few studies have dealt with distribution of infections across populations and with the influence of these bacteria on host genetic diversification and speciation. The aims of this study are to determine the distribution and rate of infection and to characterize the Wolbachia strains associated with Philaenus spumarius spittlebug (Hemiptera) by using multilocus sequencing typing (MLST) analysis and host phylogeography. The results showed that infection rate was significantly different between members of both main mitochondrial phylogenetic lineages of P. spumarius. We detected much higher infection rates of Wolbachia in P. spumarius populations from the north-east clade than the south-west clade. Moreover, the frequency of these infections varied within and outside the contact zone known from the Carpathians. Given the reproductive alterations which are often associated with this endosymbiont, Wolbachia probably maintain genetic differentiation of its hosts in its contact zone in the Carpathians. This is one of the first studies demonstrating the presence of Wolbachia across a large part of the range of insect species, including the contact zone. The spread of Wolbachia in P. spumarius populations can potentially cause speciation by compromising the potential reproductive barrier between infected and uninfected populations. We discuss possible implications of Wolbachia infection inducing cytoplasmic incompatibility in the population dynamics of this spittlebug but confirm that more studies are also required.

No MeSH data available.


Related in: MedlinePlus