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Growth and Survival of Mesorhizobium loti Inside Acanthamoeba Enhanced Its Ability to Develop More Nodules on Lotus corniculatus.

Karaś MA, Turska-Szewczuk A, Trapska D, Urbanik-Sypniewska T - Microb. Ecol. (2015)

Bottom Line: Although the association ability and the initial uptake rate of both strains were similar, recovery of viable M. huakuii MAFF303099 after 4 h postinfection decreased markedly and that of M. loti NZP2213 increased.The internalization of mesorhizobia was mediated by the mannose-dependent receptor.M. loti NZP2213 bacteria released from amoebae developed 1.5 times more nodules on Lotus corniculatus than bacteria cultivated in an amoebae-free medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland, magdalena.karas@poczta.umcs.lublin.pl.

ABSTRACT
The importance of protozoa as environmental reservoirs of pathogens is well recognized, while their impact on survival and symbiotic properties of rhizobia has not been explored. The possible survival of free-living rhizobia inside amoebae could influence bacterial abundance in the rhizosphere of legume plants and the nodulation competitiveness of microsymbionts. Two well-characterized strains of Mesorhizobium: Mesorhizobium loti NZP2213 and Mesorhizobium huakuii symbiovar loti MAFF303099 were assayed for their growth ability within the Neff strain of Acanthamoeba castellanii. Although the association ability and the initial uptake rate of both strains were similar, recovery of viable M. huakuii MAFF303099 after 4 h postinfection decreased markedly and that of M. loti NZP2213 increased. The latter strain was also able to survive prolonged co-incubation within amoebae and to self-release from the amoeba cell. The temperature 28 °C and PBS were established as optimal for the uptake of Mesorhizobium by amoebae. The internalization of mesorhizobia was mediated by the mannose-dependent receptor. M. loti NZP2213 bacteria released from amoebae developed 1.5 times more nodules on Lotus corniculatus than bacteria cultivated in an amoebae-free medium.

No MeSH data available.


Related in: MedlinePlus

A level of binding/association of Mesorhizobium spp. with A. castellanii Neff presented as a percentage of bacterial inocula (CFU = 6 × 106) vs. temperature (28 and 18 °C) and medium type (PBS vs. PYG). Results are the mean of three independent experiments performed in duplicate. Error bars represent standard deviation (n = 3)
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Fig1: A level of binding/association of Mesorhizobium spp. with A. castellanii Neff presented as a percentage of bacterial inocula (CFU = 6 × 106) vs. temperature (28 and 18 °C) and medium type (PBS vs. PYG). Results are the mean of three independent experiments performed in duplicate. Error bars represent standard deviation (n = 3)

Mentions: The association assays were performed to determine the ability of the bacteria to interact with A. castellanii. Here, the term ‘association’ is used to describe both the rhizobia that were inside the amoebae and those that were attached on the surface of Acanthamoeba. The influence of nutrients and temperature on these relationships was studied (Fig. 1). The results obtained indicated that at 28 °C, the rates of association of NZP2213 to A. castellanii were similar in PBS and in the PYG medium (76.44 % ± 3.9 and 59.86 % ± 5.3, respectively). The rate of association of strain MAFF303099 measured in PBS at 28 °C was comparable to that of NZP2213 (60.28 % ± 2.8), but it declined ca. 2.5-fold in the PYG medium. A much lower number of bacteria interacted with A. castellanii at 18 °C. In the same medium (PBS) at 28 °C, the recovery of strains NZP2213 and MAFF303099 from amoebae cells decreased ca. 5- and 4-fold, respectively. On the other hand, in the nutrient-rich medium PYG at 18 °C, the number of bacteria engulfed by the amoebae was statistically insignificant.Fig. 1


Growth and Survival of Mesorhizobium loti Inside Acanthamoeba Enhanced Its Ability to Develop More Nodules on Lotus corniculatus.

Karaś MA, Turska-Szewczuk A, Trapska D, Urbanik-Sypniewska T - Microb. Ecol. (2015)

A level of binding/association of Mesorhizobium spp. with A. castellanii Neff presented as a percentage of bacterial inocula (CFU = 6 × 106) vs. temperature (28 and 18 °C) and medium type (PBS vs. PYG). Results are the mean of three independent experiments performed in duplicate. Error bars represent standard deviation (n = 3)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4494150&req=5

Fig1: A level of binding/association of Mesorhizobium spp. with A. castellanii Neff presented as a percentage of bacterial inocula (CFU = 6 × 106) vs. temperature (28 and 18 °C) and medium type (PBS vs. PYG). Results are the mean of three independent experiments performed in duplicate. Error bars represent standard deviation (n = 3)
Mentions: The association assays were performed to determine the ability of the bacteria to interact with A. castellanii. Here, the term ‘association’ is used to describe both the rhizobia that were inside the amoebae and those that were attached on the surface of Acanthamoeba. The influence of nutrients and temperature on these relationships was studied (Fig. 1). The results obtained indicated that at 28 °C, the rates of association of NZP2213 to A. castellanii were similar in PBS and in the PYG medium (76.44 % ± 3.9 and 59.86 % ± 5.3, respectively). The rate of association of strain MAFF303099 measured in PBS at 28 °C was comparable to that of NZP2213 (60.28 % ± 2.8), but it declined ca. 2.5-fold in the PYG medium. A much lower number of bacteria interacted with A. castellanii at 18 °C. In the same medium (PBS) at 28 °C, the recovery of strains NZP2213 and MAFF303099 from amoebae cells decreased ca. 5- and 4-fold, respectively. On the other hand, in the nutrient-rich medium PYG at 18 °C, the number of bacteria engulfed by the amoebae was statistically insignificant.Fig. 1

Bottom Line: Although the association ability and the initial uptake rate of both strains were similar, recovery of viable M. huakuii MAFF303099 after 4 h postinfection decreased markedly and that of M. loti NZP2213 increased.The internalization of mesorhizobia was mediated by the mannose-dependent receptor.M. loti NZP2213 bacteria released from amoebae developed 1.5 times more nodules on Lotus corniculatus than bacteria cultivated in an amoebae-free medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland, magdalena.karas@poczta.umcs.lublin.pl.

ABSTRACT
The importance of protozoa as environmental reservoirs of pathogens is well recognized, while their impact on survival and symbiotic properties of rhizobia has not been explored. The possible survival of free-living rhizobia inside amoebae could influence bacterial abundance in the rhizosphere of legume plants and the nodulation competitiveness of microsymbionts. Two well-characterized strains of Mesorhizobium: Mesorhizobium loti NZP2213 and Mesorhizobium huakuii symbiovar loti MAFF303099 were assayed for their growth ability within the Neff strain of Acanthamoeba castellanii. Although the association ability and the initial uptake rate of both strains were similar, recovery of viable M. huakuii MAFF303099 after 4 h postinfection decreased markedly and that of M. loti NZP2213 increased. The latter strain was also able to survive prolonged co-incubation within amoebae and to self-release from the amoeba cell. The temperature 28 °C and PBS were established as optimal for the uptake of Mesorhizobium by amoebae. The internalization of mesorhizobia was mediated by the mannose-dependent receptor. M. loti NZP2213 bacteria released from amoebae developed 1.5 times more nodules on Lotus corniculatus than bacteria cultivated in an amoebae-free medium.

No MeSH data available.


Related in: MedlinePlus