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Toward angiogenesis of implanted bio-artificial liver using scaffolds with type I collagen and adipose tissue-derived stem cells.

Lee JG, Bak SY, Nahm JH, Lee SW, Min SO, Kim KS - Korean J Hepatobiliary Pancreat Surg (2015)

Bottom Line: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days.Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare.Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Yonsei University College of Medicine, Seoul, Korea.

ABSTRACT

Backgrounds/aims: Stem cell therapies for liver disease are being studied by many researchers worldwide, but scientific evidence to demonstrate the endocrinologic effects of implanted cells is insufficient, and it is unknown whether implanted cells can function as liver cells. Achieving angiogenesis, arguably the most important characteristic of the liver, is known to be quite difficult, and no practical attempts have been made to achieve this outcome. We carried out this study to observe the possibility of angiogenesis of implanted bio-artificial liver using scaffolds.

Methods: This study used adipose tissue-derived stem cells that were collected from adult patients with liver diseases with conditions similar to the liver parenchyma. Specifically, microfilaments were used to create an artificial membrane and maintain the structure of an artificial organ. After scratching the stomach surface of severe combined immunocompromised (SCID) mice (n=4), artificial scaffolds with adipose tissue-derived stem cells and type I collagen were implanted. Expression levels of angiogenesis markers including vascular endothelial growth factor (VEGF), CD34, and CD105 were immunohistochemically assessed after 30 days.

Results: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days.

Conclusions: Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare. Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.

No MeSH data available.


Related in: MedlinePlus

A CD105-stained artificial scaffold processed 30 days after implantation into SCID. The area in the black circle (A) is magnified. (D) ×100 and (F) ×200 magnifications of the area in the black dotted circle area (B). The cells inside the artificial scaffold include hematoxylin-stained adipose tissue-derived stem cells and diaminobenzidine-stained collagen. (C) ×100 magnification and (E) ×200 magnification of the area in the black circle shown in (B). The outside of the artificial scaffold contained mouse omentum cells with no collagen. There was no endothelial or vascular structure or CD105 expression.
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Figure 9: A CD105-stained artificial scaffold processed 30 days after implantation into SCID. The area in the black circle (A) is magnified. (D) ×100 and (F) ×200 magnifications of the area in the black dotted circle area (B). The cells inside the artificial scaffold include hematoxylin-stained adipose tissue-derived stem cells and diaminobenzidine-stained collagen. (C) ×100 magnification and (E) ×200 magnification of the area in the black circle shown in (B). The outside of the artificial scaffold contained mouse omentum cells with no collagen. There was no endothelial or vascular structure or CD105 expression.

Mentions: The CD105 immunohistochemistry stained slide also showed progressive brown staining starting from the membrane of the artificial scaffold (Fig. 9A, B). There were no endothelial cells or vascular structures at ×200 magnification, and the inside of the scaffold contained only stained collagen and was negative for CD105. Mouse omentum cells were observed outside the scaffold, and there was no positive staining for CD105 (Fig. 9E). On the other hand, the inside of the artificial scaffold contained hematoxylin-stained adipose tissue-derived stem cells and diaminobenzidine-stained collagen but was negative for CD105 (Fig. 9F). Microscopic observation failed to reveal any endothelial cells or vascular structures within the artificial scaffolds. Although diaminobenzidine-stained brown collagen was visible inside the scaffolds, the three angiogenesis markers were not expressed.


Toward angiogenesis of implanted bio-artificial liver using scaffolds with type I collagen and adipose tissue-derived stem cells.

Lee JG, Bak SY, Nahm JH, Lee SW, Min SO, Kim KS - Korean J Hepatobiliary Pancreat Surg (2015)

A CD105-stained artificial scaffold processed 30 days after implantation into SCID. The area in the black circle (A) is magnified. (D) ×100 and (F) ×200 magnifications of the area in the black dotted circle area (B). The cells inside the artificial scaffold include hematoxylin-stained adipose tissue-derived stem cells and diaminobenzidine-stained collagen. (C) ×100 magnification and (E) ×200 magnification of the area in the black circle shown in (B). The outside of the artificial scaffold contained mouse omentum cells with no collagen. There was no endothelial or vascular structure or CD105 expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494077&req=5

Figure 9: A CD105-stained artificial scaffold processed 30 days after implantation into SCID. The area in the black circle (A) is magnified. (D) ×100 and (F) ×200 magnifications of the area in the black dotted circle area (B). The cells inside the artificial scaffold include hematoxylin-stained adipose tissue-derived stem cells and diaminobenzidine-stained collagen. (C) ×100 magnification and (E) ×200 magnification of the area in the black circle shown in (B). The outside of the artificial scaffold contained mouse omentum cells with no collagen. There was no endothelial or vascular structure or CD105 expression.
Mentions: The CD105 immunohistochemistry stained slide also showed progressive brown staining starting from the membrane of the artificial scaffold (Fig. 9A, B). There were no endothelial cells or vascular structures at ×200 magnification, and the inside of the scaffold contained only stained collagen and was negative for CD105. Mouse omentum cells were observed outside the scaffold, and there was no positive staining for CD105 (Fig. 9E). On the other hand, the inside of the artificial scaffold contained hematoxylin-stained adipose tissue-derived stem cells and diaminobenzidine-stained collagen but was negative for CD105 (Fig. 9F). Microscopic observation failed to reveal any endothelial cells or vascular structures within the artificial scaffolds. Although diaminobenzidine-stained brown collagen was visible inside the scaffolds, the three angiogenesis markers were not expressed.

Bottom Line: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days.Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare.Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Yonsei University College of Medicine, Seoul, Korea.

ABSTRACT

Backgrounds/aims: Stem cell therapies for liver disease are being studied by many researchers worldwide, but scientific evidence to demonstrate the endocrinologic effects of implanted cells is insufficient, and it is unknown whether implanted cells can function as liver cells. Achieving angiogenesis, arguably the most important characteristic of the liver, is known to be quite difficult, and no practical attempts have been made to achieve this outcome. We carried out this study to observe the possibility of angiogenesis of implanted bio-artificial liver using scaffolds.

Methods: This study used adipose tissue-derived stem cells that were collected from adult patients with liver diseases with conditions similar to the liver parenchyma. Specifically, microfilaments were used to create an artificial membrane and maintain the structure of an artificial organ. After scratching the stomach surface of severe combined immunocompromised (SCID) mice (n=4), artificial scaffolds with adipose tissue-derived stem cells and type I collagen were implanted. Expression levels of angiogenesis markers including vascular endothelial growth factor (VEGF), CD34, and CD105 were immunohistochemically assessed after 30 days.

Results: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days.

Conclusions: Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare. Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.

No MeSH data available.


Related in: MedlinePlus