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Toward angiogenesis of implanted bio-artificial liver using scaffolds with type I collagen and adipose tissue-derived stem cells.

Lee JG, Bak SY, Nahm JH, Lee SW, Min SO, Kim KS - Korean J Hepatobiliary Pancreat Surg (2015)

Bottom Line: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days.Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare.Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Yonsei University College of Medicine, Seoul, Korea.

ABSTRACT

Backgrounds/aims: Stem cell therapies for liver disease are being studied by many researchers worldwide, but scientific evidence to demonstrate the endocrinologic effects of implanted cells is insufficient, and it is unknown whether implanted cells can function as liver cells. Achieving angiogenesis, arguably the most important characteristic of the liver, is known to be quite difficult, and no practical attempts have been made to achieve this outcome. We carried out this study to observe the possibility of angiogenesis of implanted bio-artificial liver using scaffolds.

Methods: This study used adipose tissue-derived stem cells that were collected from adult patients with liver diseases with conditions similar to the liver parenchyma. Specifically, microfilaments were used to create an artificial membrane and maintain the structure of an artificial organ. After scratching the stomach surface of severe combined immunocompromised (SCID) mice (n=4), artificial scaffolds with adipose tissue-derived stem cells and type I collagen were implanted. Expression levels of angiogenesis markers including vascular endothelial growth factor (VEGF), CD34, and CD105 were immunohistochemically assessed after 30 days.

Results: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days.

Conclusions: Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare. Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.

No MeSH data available.


Related in: MedlinePlus

Magnification of the inner side of the H&E-stained artificial scaffold 30 days after implantation into SCID mice. The area in the black circle (A) is magnified in (B-D). (D) The ×200 magnification shows eosin-stained collagen and hematoxylin-stained adipose tissue-derived stem cells within the artificial scaffold.
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Figure 6: Magnification of the inner side of the H&E-stained artificial scaffold 30 days after implantation into SCID mice. The area in the black circle (A) is magnified in (B-D). (D) The ×200 magnification shows eosin-stained collagen and hematoxylin-stained adipose tissue-derived stem cells within the artificial scaffold.

Mentions: We next observed the H&E-stained artificial scaffolds under high magnification to better assess its characteristics (Figs. 5, 6). The basement membrane surrounding the outer side of the artificial scaffold contained eosin-stained collagen without nuclei (Fig. 5), and we also observed hematoxylin-stained adipose tissue-derived stem cells within the artificial scaffold (Fig. 6).


Toward angiogenesis of implanted bio-artificial liver using scaffolds with type I collagen and adipose tissue-derived stem cells.

Lee JG, Bak SY, Nahm JH, Lee SW, Min SO, Kim KS - Korean J Hepatobiliary Pancreat Surg (2015)

Magnification of the inner side of the H&E-stained artificial scaffold 30 days after implantation into SCID mice. The area in the black circle (A) is magnified in (B-D). (D) The ×200 magnification shows eosin-stained collagen and hematoxylin-stained adipose tissue-derived stem cells within the artificial scaffold.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494077&req=5

Figure 6: Magnification of the inner side of the H&E-stained artificial scaffold 30 days after implantation into SCID mice. The area in the black circle (A) is magnified in (B-D). (D) The ×200 magnification shows eosin-stained collagen and hematoxylin-stained adipose tissue-derived stem cells within the artificial scaffold.
Mentions: We next observed the H&E-stained artificial scaffolds under high magnification to better assess its characteristics (Figs. 5, 6). The basement membrane surrounding the outer side of the artificial scaffold contained eosin-stained collagen without nuclei (Fig. 5), and we also observed hematoxylin-stained adipose tissue-derived stem cells within the artificial scaffold (Fig. 6).

Bottom Line: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days.Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare.Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Yonsei University College of Medicine, Seoul, Korea.

ABSTRACT

Backgrounds/aims: Stem cell therapies for liver disease are being studied by many researchers worldwide, but scientific evidence to demonstrate the endocrinologic effects of implanted cells is insufficient, and it is unknown whether implanted cells can function as liver cells. Achieving angiogenesis, arguably the most important characteristic of the liver, is known to be quite difficult, and no practical attempts have been made to achieve this outcome. We carried out this study to observe the possibility of angiogenesis of implanted bio-artificial liver using scaffolds.

Methods: This study used adipose tissue-derived stem cells that were collected from adult patients with liver diseases with conditions similar to the liver parenchyma. Specifically, microfilaments were used to create an artificial membrane and maintain the structure of an artificial organ. After scratching the stomach surface of severe combined immunocompromised (SCID) mice (n=4), artificial scaffolds with adipose tissue-derived stem cells and type I collagen were implanted. Expression levels of angiogenesis markers including vascular endothelial growth factor (VEGF), CD34, and CD105 were immunohistochemically assessed after 30 days.

Results: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days.

Conclusions: Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare. Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.

No MeSH data available.


Related in: MedlinePlus