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Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes.

Barghi N, Concepcion GP, Olivera BM, Lluisma AO - Genome Biol Evol (2015)

Bottom Line: In this study, the sequences and expression levels of conotoxins from several specimens of two closely related worm-hunting species, Conus tribblei and Conus lenavati, were compared through transcriptome analysis.Comparison of the interspecific expression patterns of conopeptides at the superfamily level resulted in the discovery of both conserved as well as species-specific expression patterns, indicating divergence in the regulatory network affecting conotoxin gene expression.Comparison of the transcriptomes of the individual snails revealed that each specimen produces a distinct set of highly expressed conopeptides, reflecting the capability of individual snails to fine-tune the composition of their venoms.

View Article: PubMed Central - PubMed

Affiliation: Marine Science Institute, University of the Philippines-Diliman, Quezon City, Philippines.

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The expression of orthologous conopeptide pairs in C. tribblei and C. lenavati. (a) The correlation of the expression levels of identical orthologous conopeptide pairs (r = 0.614, P = 0.003). The correlation of the expression of orthologs pairs except for the three conopeptides with divergent expression patterns (marked with black arrows) (r = 0.924, P value < 0.001) is shown in insert. The correlation of orthologous conopeptide pairs (b) showing dN to dS ratios < 1 (r = 0.749, P = 0.02) and (c) dN to dS ratios > 1 (r = 0.585, P value < 0.001). The abbreviations of superfamily names are noted in figure 2.
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evv109-F6: The expression of orthologous conopeptide pairs in C. tribblei and C. lenavati. (a) The correlation of the expression levels of identical orthologous conopeptide pairs (r = 0.614, P = 0.003). The correlation of the expression of orthologs pairs except for the three conopeptides with divergent expression patterns (marked with black arrows) (r = 0.924, P value < 0.001) is shown in insert. The correlation of orthologous conopeptide pairs (b) showing dN to dS ratios < 1 (r = 0.749, P = 0.02) and (c) dN to dS ratios > 1 (r = 0.585, P value < 0.001). The abbreviations of superfamily names are noted in figure 2.

Mentions: The conopeptides with identical mature regions belonged to 14 superfamilies (B1-, N-, O1-, O2-, P-, W- and Y-superfamilies, “divergent MKFPLLFISL” superfamily, con-ikot-ikot and conopressin/conophysin families, and A-like, Y2-like, SF-02 and SF-04 groups) (fig. 6a). The expression levels of all orthologous conopeptide gene pairs in C. tribblei and C. lenavati were moderately correlated (r = 0.640, P value < 0.001, data not shown), and the correlation of the expression of identical orthologous conopeptide genes was slightly lower (r = 0.614, P = 0.003) (fig. 6a). Notably, the expressions of three outliers (conopeptides of O1- and O2- and P-superfamilies) (fig. 6a) in the correlation test of identical orthologs were highly divergent that upon their exclusion, the correlation increased to r = 0.924 (P value < 0.001). The expression of orthologs having higher rate of dS to dN was moderately though not significantly correlated (r = 0.749, P = 0.02) (fig. 6b), whereas the expression of orthologous pairs under positive selection showed weak correlation (r = 0.585, P value < 0.001) (fig. 6c).Fig. 6.—


Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes.

Barghi N, Concepcion GP, Olivera BM, Lluisma AO - Genome Biol Evol (2015)

The expression of orthologous conopeptide pairs in C. tribblei and C. lenavati. (a) The correlation of the expression levels of identical orthologous conopeptide pairs (r = 0.614, P = 0.003). The correlation of the expression of orthologs pairs except for the three conopeptides with divergent expression patterns (marked with black arrows) (r = 0.924, P value < 0.001) is shown in insert. The correlation of orthologous conopeptide pairs (b) showing dN to dS ratios < 1 (r = 0.749, P = 0.02) and (c) dN to dS ratios > 1 (r = 0.585, P value < 0.001). The abbreviations of superfamily names are noted in figure 2.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4494072&req=5

evv109-F6: The expression of orthologous conopeptide pairs in C. tribblei and C. lenavati. (a) The correlation of the expression levels of identical orthologous conopeptide pairs (r = 0.614, P = 0.003). The correlation of the expression of orthologs pairs except for the three conopeptides with divergent expression patterns (marked with black arrows) (r = 0.924, P value < 0.001) is shown in insert. The correlation of orthologous conopeptide pairs (b) showing dN to dS ratios < 1 (r = 0.749, P = 0.02) and (c) dN to dS ratios > 1 (r = 0.585, P value < 0.001). The abbreviations of superfamily names are noted in figure 2.
Mentions: The conopeptides with identical mature regions belonged to 14 superfamilies (B1-, N-, O1-, O2-, P-, W- and Y-superfamilies, “divergent MKFPLLFISL” superfamily, con-ikot-ikot and conopressin/conophysin families, and A-like, Y2-like, SF-02 and SF-04 groups) (fig. 6a). The expression levels of all orthologous conopeptide gene pairs in C. tribblei and C. lenavati were moderately correlated (r = 0.640, P value < 0.001, data not shown), and the correlation of the expression of identical orthologous conopeptide genes was slightly lower (r = 0.614, P = 0.003) (fig. 6a). Notably, the expressions of three outliers (conopeptides of O1- and O2- and P-superfamilies) (fig. 6a) in the correlation test of identical orthologs were highly divergent that upon their exclusion, the correlation increased to r = 0.924 (P value < 0.001). The expression of orthologs having higher rate of dS to dN was moderately though not significantly correlated (r = 0.749, P = 0.02) (fig. 6b), whereas the expression of orthologous pairs under positive selection showed weak correlation (r = 0.585, P value < 0.001) (fig. 6c).Fig. 6.—

Bottom Line: In this study, the sequences and expression levels of conotoxins from several specimens of two closely related worm-hunting species, Conus tribblei and Conus lenavati, were compared through transcriptome analysis.Comparison of the interspecific expression patterns of conopeptides at the superfamily level resulted in the discovery of both conserved as well as species-specific expression patterns, indicating divergence in the regulatory network affecting conotoxin gene expression.Comparison of the transcriptomes of the individual snails revealed that each specimen produces a distinct set of highly expressed conopeptides, reflecting the capability of individual snails to fine-tune the composition of their venoms.

View Article: PubMed Central - PubMed

Affiliation: Marine Science Institute, University of the Philippines-Diliman, Quezon City, Philippines.

Show MeSH
Related in: MedlinePlus