Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes.
Bottom Line: In this study, the sequences and expression levels of conotoxins from several specimens of two closely related worm-hunting species, Conus tribblei and Conus lenavati, were compared through transcriptome analysis.Comparison of the interspecific expression patterns of conopeptides at the superfamily level resulted in the discovery of both conserved as well as species-specific expression patterns, indicating divergence in the regulatory network affecting conotoxin gene expression.Comparison of the transcriptomes of the individual snails revealed that each specimen produces a distinct set of highly expressed conopeptides, reflecting the capability of individual snails to fine-tune the composition of their venoms.
Affiliation: Marine Science Institute, University of the Philippines-Diliman, Quezon City, Philippines.Show MeSH
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Mentions: The expression patterns of all the conopeptide superfamilies were moderately correlated between C. tribblei and C. lenavati (r = 0.608, P value < 0.001) (fig. 4), but it was apparent that a great number of superfamilies had highly correlated expression (32 shared superfamilies, r = 0.820, P value < 0.001). Although the expression patterns of majority of these superfamilies were apparently conserved, another subset consisting of the S-, H-, and U-superfamilies, and the conkunitzin family had very different expression levels in C. tribblei and C. lenavati (fig. 4 and supplementary table S4, Supplementary Material online). There is moderate correlation between the abundance and expression of different conopeptide gene superfamilies in C. tribblei (r = 0.602, P value < 0.001) and C. lenavati (r = 0.597, P value < 0.001) (supplementary fig. S2, Supplementary Material online).Fig. 4.—
Affiliation: Marine Science Institute, University of the Philippines-Diliman, Quezon City, Philippines.