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Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes.

Barghi N, Concepcion GP, Olivera BM, Lluisma AO - Genome Biol Evol (2015)

Bottom Line: In this study, the sequences and expression levels of conotoxins from several specimens of two closely related worm-hunting species, Conus tribblei and Conus lenavati, were compared through transcriptome analysis.Comparison of the interspecific expression patterns of conopeptides at the superfamily level resulted in the discovery of both conserved as well as species-specific expression patterns, indicating divergence in the regulatory network affecting conotoxin gene expression.Comparison of the transcriptomes of the individual snails revealed that each specimen produces a distinct set of highly expressed conopeptides, reflecting the capability of individual snails to fine-tune the composition of their venoms.

View Article: PubMed Central - PubMed

Affiliation: Marine Science Institute, University of the Philippines-Diliman, Quezon City, Philippines.

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Phylogenetic relationship of the species of subgenus Splinoconus. The phylogenetic tree is inferred from the partial COI sequences using Bayesian analysis. Posterior probabilities are indicated for each node. The specimens of C. tribblei and C. lenavati used for the transcriptome analysis are marked with red asterisks.
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evv109-F1: Phylogenetic relationship of the species of subgenus Splinoconus. The phylogenetic tree is inferred from the partial COI sequences using Bayesian analysis. Posterior probabilities are indicated for each node. The specimens of C. tribblei and C. lenavati used for the transcriptome analysis are marked with red asterisks.

Mentions: The mRNA extraction was performed individually on the venom ducts of three specimens of C. tribblei (C. tribblei_0317_1 [trib1, female], C. tribblei_0317_3 [trib3, male], and C. tribblei_0106_21 [trib21, male]) and three specimens of C. lenavati (C. lenavati_0317_1 [lena1, male], C. lenavati_0317_2 [lena2, female], and C. lenavati_0317_3 [lena3, male]) (fig. 1) using Dynabeads mRNA DIRECT kit (Invitrogen Dynal AS Oslo, Norway). For a more efficient extraction, each venom duct was homogenized using 0.5-mm Zirconia/Silica beads (Biospec Products, Inc.) in a bead beater (Precellys, Berlin Technologies), and the rest of the protocol was performed according to the manufacturer’s recommendations. A cDNA library was constructed for each of the specimens, fragments with the average insert size of 200 bp were selected, and the libraries were multiplexed and paired-end (PE) sequenced using Illumina HiSeq 2000.Fig. 1.—


Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes.

Barghi N, Concepcion GP, Olivera BM, Lluisma AO - Genome Biol Evol (2015)

Phylogenetic relationship of the species of subgenus Splinoconus. The phylogenetic tree is inferred from the partial COI sequences using Bayesian analysis. Posterior probabilities are indicated for each node. The specimens of C. tribblei and C. lenavati used for the transcriptome analysis are marked with red asterisks.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494072&req=5

evv109-F1: Phylogenetic relationship of the species of subgenus Splinoconus. The phylogenetic tree is inferred from the partial COI sequences using Bayesian analysis. Posterior probabilities are indicated for each node. The specimens of C. tribblei and C. lenavati used for the transcriptome analysis are marked with red asterisks.
Mentions: The mRNA extraction was performed individually on the venom ducts of three specimens of C. tribblei (C. tribblei_0317_1 [trib1, female], C. tribblei_0317_3 [trib3, male], and C. tribblei_0106_21 [trib21, male]) and three specimens of C. lenavati (C. lenavati_0317_1 [lena1, male], C. lenavati_0317_2 [lena2, female], and C. lenavati_0317_3 [lena3, male]) (fig. 1) using Dynabeads mRNA DIRECT kit (Invitrogen Dynal AS Oslo, Norway). For a more efficient extraction, each venom duct was homogenized using 0.5-mm Zirconia/Silica beads (Biospec Products, Inc.) in a bead beater (Precellys, Berlin Technologies), and the rest of the protocol was performed according to the manufacturer’s recommendations. A cDNA library was constructed for each of the specimens, fragments with the average insert size of 200 bp were selected, and the libraries were multiplexed and paired-end (PE) sequenced using Illumina HiSeq 2000.Fig. 1.—

Bottom Line: In this study, the sequences and expression levels of conotoxins from several specimens of two closely related worm-hunting species, Conus tribblei and Conus lenavati, were compared through transcriptome analysis.Comparison of the interspecific expression patterns of conopeptides at the superfamily level resulted in the discovery of both conserved as well as species-specific expression patterns, indicating divergence in the regulatory network affecting conotoxin gene expression.Comparison of the transcriptomes of the individual snails revealed that each specimen produces a distinct set of highly expressed conopeptides, reflecting the capability of individual snails to fine-tune the composition of their venoms.

View Article: PubMed Central - PubMed

Affiliation: Marine Science Institute, University of the Philippines-Diliman, Quezon City, Philippines.

Show MeSH