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Differential Expression of Genes that Control Respiration Contribute to Thermal Adaptation in Redband Trout (Oncorhynchus mykiss gairdneri).

Garvin MR, Thorgaard GH, Narum SR - Genome Biol Evol (2015)

Bottom Line: Because increased water temperatures will reduce the dissolved oxygen available for fish, we hypothesized that adaptation to low oxygen environments would involve improved respiration through oxidative phosphorylation (OXPHOS).To test this hypothesis, we subjected individuals from two ecologically divergent populations of inland (redband) rainbow trout (Oncorhynchus mykiss gairdneri) with historically different temperature regimes (desert and montane) and their F1 progeny to diel cycles of temperature stress and then examined gene expression data for 80 nuclear- and mitochondrial-encoded OXPHOS subunits that participate in respiration.Supercomplexes may increase the efficiency of respiration because complexes I, III, and IV are brought into close proximity and oligomerization of complex V alters the macrostructure of mitochondria to improve respiration.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, Washington State University michael.garvin@wsu.edu.

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A three-dimensional rendering of the supercomplex formed by complexes I, III, and IV. Each subunit is represented by a different color in each complex (top). Subunits that are differentially regulated in the desert strain as part of the response to increased temperature are shown in white (bottom).
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evv078-F2: A three-dimensional rendering of the supercomplex formed by complexes I, III, and IV. Each subunit is represented by a different color in each complex (top). Subunits that are differentially regulated in the desert strain as part of the response to increased temperature are shown in white (bottom).

Mentions: The COX6B2 isoform was upregulated in the desert fish in response to temperature. COX6B lies at the interface between monomers of complex IV and is one of the subunits that initiates the formation of the active, functional homodimer (Pierron et al. 2012). Different isoforms of nuclear-encoded COX subunits have been shown in many species to be important for the regulation of respiration and confer different activities on complex IV in different tissues and under different oxygen concentrations (Arnold 2012a; Pierron et al. 2012). It is possible that switching from the 6B1 to the 6B2 isoform here in gill tissue alters the formation of the homodimer, increases the stability of the complex itself or stabilizes interactions with other complexes, that is, supercomplexes (fig. 2) (Dudkina et al. 2008).Fig. 2.—


Differential Expression of Genes that Control Respiration Contribute to Thermal Adaptation in Redband Trout (Oncorhynchus mykiss gairdneri).

Garvin MR, Thorgaard GH, Narum SR - Genome Biol Evol (2015)

A three-dimensional rendering of the supercomplex formed by complexes I, III, and IV. Each subunit is represented by a different color in each complex (top). Subunits that are differentially regulated in the desert strain as part of the response to increased temperature are shown in white (bottom).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494065&req=5

evv078-F2: A three-dimensional rendering of the supercomplex formed by complexes I, III, and IV. Each subunit is represented by a different color in each complex (top). Subunits that are differentially regulated in the desert strain as part of the response to increased temperature are shown in white (bottom).
Mentions: The COX6B2 isoform was upregulated in the desert fish in response to temperature. COX6B lies at the interface between monomers of complex IV and is one of the subunits that initiates the formation of the active, functional homodimer (Pierron et al. 2012). Different isoforms of nuclear-encoded COX subunits have been shown in many species to be important for the regulation of respiration and confer different activities on complex IV in different tissues and under different oxygen concentrations (Arnold 2012a; Pierron et al. 2012). It is possible that switching from the 6B1 to the 6B2 isoform here in gill tissue alters the formation of the homodimer, increases the stability of the complex itself or stabilizes interactions with other complexes, that is, supercomplexes (fig. 2) (Dudkina et al. 2008).Fig. 2.—

Bottom Line: Because increased water temperatures will reduce the dissolved oxygen available for fish, we hypothesized that adaptation to low oxygen environments would involve improved respiration through oxidative phosphorylation (OXPHOS).To test this hypothesis, we subjected individuals from two ecologically divergent populations of inland (redband) rainbow trout (Oncorhynchus mykiss gairdneri) with historically different temperature regimes (desert and montane) and their F1 progeny to diel cycles of temperature stress and then examined gene expression data for 80 nuclear- and mitochondrial-encoded OXPHOS subunits that participate in respiration.Supercomplexes may increase the efficiency of respiration because complexes I, III, and IV are brought into close proximity and oligomerization of complex V alters the macrostructure of mitochondria to improve respiration.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, Washington State University michael.garvin@wsu.edu.

Show MeSH
Related in: MedlinePlus