Functionally Structured Genomes in Lactobacillus kunkeei Colonizing the Honey Crop and Food Products of Honeybees and Stingless Bees.
Bottom Line: A gene flux analysis, including previously sequenced Lactobacillus species as outgroups, indicated the influence of reductive evolution.We suggest that these features have resulted from a genome-wide loss of genes, with integrations of novel genes mostly occurring in regions flanking the origin of replication.The results provide an extended framework for reductive genome evolution and functional genome organization in bacteria.
Affiliation: Department of Molecular Evolution, Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Sweden.Show MeSH
Mentions: To determine whether L. kunkeei has codiversified with the bees, we compared the tree topologies of bacteria and bees. On the host side, it has been suggested that dwarf bees, giant bees, cavity nesting bees, and stingless bees belong to four different monophyletic groups (Arias and Sheppard 2005; Raffiudin and Crozier 2007; Lo et al. 2010). Our comparison of the diversification patterns revealed no congruence between bacterial and host tree topologies (fig. 7), suggesting that the L. kunkeei strains have not codiversified with their hosts. Nor did we find any correlation between strain relationships and country of isolation.Fig. 7.—
Affiliation: Department of Molecular Evolution, Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Sweden.