Functionally Structured Genomes in Lactobacillus kunkeei Colonizing the Honey Crop and Food Products of Honeybees and Stingless Bees.
Bottom Line: A gene flux analysis, including previously sequenced Lactobacillus species as outgroups, indicated the influence of reductive evolution.We suggest that these features have resulted from a genome-wide loss of genes, with integrations of novel genes mostly occurring in regions flanking the origin of replication.The results provide an extended framework for reductive genome evolution and functional genome organization in bacteria.
Affiliation: Department of Molecular Evolution, Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Sweden.Show MeSH
Mentions: A visual inspection of the genome architecture in strain Fhon2 indicated that there was an exceptional bias in the functional organization of genes, such that conserved single-copy orthologs present in all of the currently sequenced Lactobacillus genomes were mainly situated in the chromosomal half that is flanking ter (fig. 3). In contrast, genes for metabolic processes tended to be located in the genomic half that flanks the origin of replication (fig. 3). The genes highly represented in this region code for proteins related to the metabolism of amino acids, such as ABC transporters, permeases, peptidases, and proteases.Fig. 3.—
Affiliation: Department of Molecular Evolution, Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Sweden.