Functionally Structured Genomes in Lactobacillus kunkeei Colonizing the Honey Crop and Food Products of Honeybees and Stingless Bees.
Bottom Line: A gene flux analysis, including previously sequenced Lactobacillus species as outgroups, indicated the influence of reductive evolution.We suggest that these features have resulted from a genome-wide loss of genes, with integrations of novel genes mostly occurring in regions flanking the origin of replication.The results provide an extended framework for reductive genome evolution and functional genome organization in bacteria.
Affiliation: Department of Molecular Evolution, Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Sweden.Show MeSH
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Mentions: A comparison of a 1,408-bp-long alignment of the 16 S rRNA gene showed that eight of the isolated strains were identical to the type strain, L. kunkeei YH15, whereas strain LAdo contained a single polymorphism, and strains LAko and LAnu shared another polymorphism. Strain Fhon13 showed 98.8% sequence identity (17 polymorphisms) in the 16 S rRNA gene to L. kunkeei YH-15. An rRNA-based maximum-likelihood phylogeny showed that L. kunkeei and L. apinorum Fhon13 were related to Lactobacillus ozensis, Lactobacillus lindneri, Lactobacillus sanfranciscensis, and Lactobacillus fructivorans (fig. 1a). For the purpose of this discussion, we have considered L. apinorum Fhon13 to be a member of the L. kunkeei species complex.Fig. 1.—
Affiliation: Department of Molecular Evolution, Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Sweden.