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Alarmingly High Segregation Frequencies of Quinolone Resistance Alleles within Human and Animal Microbiomes Are Not Explained by Direct Clinical Antibiotic Exposure.

Field W, Hershberg R - Genome Biol Evol (2015)

Bottom Line: Within host-associated environments, resistance to quinolones was most often conferred by a specific resistance allele.High frequencies of quinolone resistance alleles were also found within hosts that were not directly treated with antibiotics.Therefore, the high segregation frequency of quinolone resistance alleles occurring within the housekeeping targets of antibiotics in host-associated environments does not seem to be the sole result of clinical antibiotic usage.

View Article: PubMed Central - PubMed

Affiliation: Rachel & Menachem Mendelovitch Evolutionary Processes of Mutation & Natural Selection Research Laboratory, Department of Genetics and Developmental Biology, the Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.

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Frequency of TRAs varies greatly between different bacterial phyla. Depicted are the average frequencies with which GyrA/ParC (green), RpoB (red), and RpsL (blue) protein sequences carry resistance alleles for the five most prevalent phyla. These frequencies are calculated based on combined data from all environments sampled.
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evv102-F3: Frequency of TRAs varies greatly between different bacterial phyla. Depicted are the average frequencies with which GyrA/ParC (green), RpoB (red), and RpsL (blue) protein sequences carry resistance alleles for the five most prevalent phyla. These frequencies are calculated based on combined data from all environments sampled.

Mentions: Following phylogenetic classifications of sequences of the three housekeeping genes, we could examine the frequency of TRAs within each phyla separately (fig. 3). We find that different phyla vary in overall frequencies of TRAs (fig. 3). While also present for the other antibiotic classes, this variation in TRA frequency is most noticeable in the case of quinolone resistance. GyrA/ParC sequences classified as belonging to Fusobacteria, Bacteroidetes, and Firmicutes carry quinolone TRAs much more frequently than sequences classified as coming from Proteobacteria or Actinobacteria (fig. 3).Fig. 3.—


Alarmingly High Segregation Frequencies of Quinolone Resistance Alleles within Human and Animal Microbiomes Are Not Explained by Direct Clinical Antibiotic Exposure.

Field W, Hershberg R - Genome Biol Evol (2015)

Frequency of TRAs varies greatly between different bacterial phyla. Depicted are the average frequencies with which GyrA/ParC (green), RpoB (red), and RpsL (blue) protein sequences carry resistance alleles for the five most prevalent phyla. These frequencies are calculated based on combined data from all environments sampled.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494058&req=5

evv102-F3: Frequency of TRAs varies greatly between different bacterial phyla. Depicted are the average frequencies with which GyrA/ParC (green), RpoB (red), and RpsL (blue) protein sequences carry resistance alleles for the five most prevalent phyla. These frequencies are calculated based on combined data from all environments sampled.
Mentions: Following phylogenetic classifications of sequences of the three housekeeping genes, we could examine the frequency of TRAs within each phyla separately (fig. 3). We find that different phyla vary in overall frequencies of TRAs (fig. 3). While also present for the other antibiotic classes, this variation in TRA frequency is most noticeable in the case of quinolone resistance. GyrA/ParC sequences classified as belonging to Fusobacteria, Bacteroidetes, and Firmicutes carry quinolone TRAs much more frequently than sequences classified as coming from Proteobacteria or Actinobacteria (fig. 3).Fig. 3.—

Bottom Line: Within host-associated environments, resistance to quinolones was most often conferred by a specific resistance allele.High frequencies of quinolone resistance alleles were also found within hosts that were not directly treated with antibiotics.Therefore, the high segregation frequency of quinolone resistance alleles occurring within the housekeeping targets of antibiotics in host-associated environments does not seem to be the sole result of clinical antibiotic usage.

View Article: PubMed Central - PubMed

Affiliation: Rachel & Menachem Mendelovitch Evolutionary Processes of Mutation & Natural Selection Research Laboratory, Department of Genetics and Developmental Biology, the Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.

Show MeSH