Limits...
Alarmingly High Segregation Frequencies of Quinolone Resistance Alleles within Human and Animal Microbiomes Are Not Explained by Direct Clinical Antibiotic Exposure.

Field W, Hershberg R - Genome Biol Evol (2015)

Bottom Line: Within host-associated environments, resistance to quinolones was most often conferred by a specific resistance allele.High frequencies of quinolone resistance alleles were also found within hosts that were not directly treated with antibiotics.Therefore, the high segregation frequency of quinolone resistance alleles occurring within the housekeeping targets of antibiotics in host-associated environments does not seem to be the sole result of clinical antibiotic usage.

View Article: PubMed Central - PubMed

Affiliation: Rachel & Menachem Mendelovitch Evolutionary Processes of Mutation & Natural Selection Research Laboratory, Department of Genetics and Developmental Biology, the Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.

Show MeSH

Related in: MedlinePlus

Breakdown of quinolone and streptomycin TRAs among sequences carrying a TRA. Depicted are three pie charts for each antibiotic class, representing the distribution of TRAs within all environments, host-associated environments, and non host-associated environments. n denotes the total number of sequences with TRAs in each group.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4494058&req=5

evv102-F2: Breakdown of quinolone and streptomycin TRAs among sequences carrying a TRA. Depicted are three pie charts for each antibiotic class, representing the distribution of TRAs within all environments, host-associated environments, and non host-associated environments. n denotes the total number of sequences with TRAs in each group.

Mentions: The first striking result of these analyses is the observation of a notable frequency of TRAs to all three antibiotics across most environments (fig. 1A). Rifamycin TRAs are segregated at the lowest frequencies. The frequency of RpoB sequences carrying a rifamycin TRA is on average 0.49% across environments and ranges between 0% and 1.70% for the different environments analyzed (fig. 1A). RpsL sequences carry resistance alleles to streptomycin with an average frequency of 7.44% across studied environments, ranging between 0% and 15.10% (fig. 1A). Across all environments sampled, resistance to streptomycin was conferred almost exclusively by a single resistance allele, arginine at RpsL position 88 (88R, position numbers are given in reference to the E. coli amino acid sequence, fig. 2). Quinolone TRAs segregate at the highest frequencies, with an average frequency across environments of 17.78%, ranging between 2.12% and 68.87% (fig. 1A).Fig. 1.—


Alarmingly High Segregation Frequencies of Quinolone Resistance Alleles within Human and Animal Microbiomes Are Not Explained by Direct Clinical Antibiotic Exposure.

Field W, Hershberg R - Genome Biol Evol (2015)

Breakdown of quinolone and streptomycin TRAs among sequences carrying a TRA. Depicted are three pie charts for each antibiotic class, representing the distribution of TRAs within all environments, host-associated environments, and non host-associated environments. n denotes the total number of sequences with TRAs in each group.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494058&req=5

evv102-F2: Breakdown of quinolone and streptomycin TRAs among sequences carrying a TRA. Depicted are three pie charts for each antibiotic class, representing the distribution of TRAs within all environments, host-associated environments, and non host-associated environments. n denotes the total number of sequences with TRAs in each group.
Mentions: The first striking result of these analyses is the observation of a notable frequency of TRAs to all three antibiotics across most environments (fig. 1A). Rifamycin TRAs are segregated at the lowest frequencies. The frequency of RpoB sequences carrying a rifamycin TRA is on average 0.49% across environments and ranges between 0% and 1.70% for the different environments analyzed (fig. 1A). RpsL sequences carry resistance alleles to streptomycin with an average frequency of 7.44% across studied environments, ranging between 0% and 15.10% (fig. 1A). Across all environments sampled, resistance to streptomycin was conferred almost exclusively by a single resistance allele, arginine at RpsL position 88 (88R, position numbers are given in reference to the E. coli amino acid sequence, fig. 2). Quinolone TRAs segregate at the highest frequencies, with an average frequency across environments of 17.78%, ranging between 2.12% and 68.87% (fig. 1A).Fig. 1.—

Bottom Line: Within host-associated environments, resistance to quinolones was most often conferred by a specific resistance allele.High frequencies of quinolone resistance alleles were also found within hosts that were not directly treated with antibiotics.Therefore, the high segregation frequency of quinolone resistance alleles occurring within the housekeeping targets of antibiotics in host-associated environments does not seem to be the sole result of clinical antibiotic usage.

View Article: PubMed Central - PubMed

Affiliation: Rachel & Menachem Mendelovitch Evolutionary Processes of Mutation & Natural Selection Research Laboratory, Department of Genetics and Developmental Biology, the Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.

Show MeSH
Related in: MedlinePlus