The Origin and Evolution of the Plant Cell Surface: Algal Integrin-Associated Proteins and a New Family of Integrin-Like Cytoskeleton-ECM Linker Proteins.
Bottom Line: The extracellular matrix of scaly green flagellates consists of small organic scales consisting of polysaccharides and scale-associated proteins (SAPs).Gralins are absent from embryophytes.A model for the evolution of the cell surface proteins in Plantae is discussed.
Affiliation: Biozentrum Köln, Botanical Institute, Universität zu Köln, Germany email@example.com.Show MeSH
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Mentions: SAPs were isolated by 2D-gel electrophoresis (under non-reducing/reducing conditions) as described by Becker et al. (1996). SAPs were sequenced by mass spectrometry. Sequences of several peptides were obtained for each of four different SAPs (supplementary table S1, Supplementary Material online). Database searches (NCBI proteins and expressed sequence tags [ESTs]) failed to identify any protein showing similarity to more than a single SAP peptide in its sequence. Standard PCR using degenerate forward and reverse primers for each peptide also failed to isolate cDNA or genomic fragments coding for any SAP. However, amplification of a short fragment of known length representing a single peptide using short oligonucleotide primers was successful in a single case for a SAP98 peptide (fig. 1A).Fig. 1.—
Affiliation: Biozentrum Köln, Botanical Institute, Universität zu Köln, Germany firstname.lastname@example.org.