The Recent De Novo Origin of Protein C-Termini.
Bottom Line: Because we study recent additions to potentially old genes, we are able to apply a variety of stringent quality filters to our annotations of what is a true protein-coding gene, discarding the putative proteins of unknown function that are typical of recent fully de novo genes.We identify 54 examples of C-terminal extensions in Saccharomyces and 28 in Drosophila, all of them recent enough to still be polymorphic.Four of the Saccharomyces C-terminal extensions (to ADH1, ARP8, TPM2, and PIS1) that survived our quality filters are predicted to lead to significant modification of a protein domain structure.
Affiliation: Department of Ecology & Evolutionary Biology, University of Arizona Present address: Aegis Sciences, Nashville, TN.Show MeSH
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Mentions: The distributions of additions across strains of S. cerevisiae and S. paradoxus are shown in figure 2. A significant number of addition alleles have risen to high frequency. Forty-eight percent (12 out of 25) addition alleles sit neatly on monophyletic clades within the tree of strains in S. paradoxus (Fig. 2A), whereas only 34% (11 out of 32) are found to be monophyletic in S. cerevisiae (fig. 2B). Those additions that are not monophyletic are widely dispersed across our sampled populations, especially in S. cerevisiae (fig. 2B). This is consistent with previous observations of greater population structure in S. paradoxus (Liti et al. 2009).Fig. 2.—
Affiliation: Department of Ecology & Evolutionary Biology, University of Arizona Present address: Aegis Sciences, Nashville, TN.