Complete Genome Sequence and Transcriptomic Analysis of the Novel Pathogen Elizabethkingia anophelis in Response to Oxidative Stress.
Bottom Line: Chrome azurol sulfonate assay verified that siderophore production of E. anophelis is increased in the presence of oxidative stress.We further showed that hemoglobin facilitates the growth, hydrogen peroxide tolerance, cell attachment, and biofilm formation of E. anophelis NUHP1.Our study suggests that siderophore production and heme uptake pathways might play essential roles in stress response and virulence of the emerging pathogen E. anophelis.
Affiliation: School of Biological Sciences, Nanyang Technological University, Singapore.Show MeSH
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Mentions: The genome of E. anophelis NUHP1 strain consists of a circular chromosome comprising 4,369,828 bp and average GC content of 35.62% (fig. 1). A total of 4,141 genes are predicted from the NUHP1 genome, where 4,074 of which are protein-coding genes and 67 of which are RNA-coding genes (52 tRNAs, 10 rRNAs, and 5 other RNAs). A total of 2,489 of the protein-coding genes can be assigned to a putative function with the remaining annotated as hypothetical proteins.Fig. 1.—
Affiliation: School of Biological Sciences, Nanyang Technological University, Singapore.