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Complete Genome Sequence and Transcriptomic Analysis of the Novel Pathogen Elizabethkingia anophelis in Response to Oxidative Stress.

Li Y, Liu Y, Chew SC, Tay M, Salido MM, Teo J, Lauro FM, Givskov M, Yang L - Genome Biol Evol (2015)

Bottom Line: Chrome azurol sulfonate assay verified that siderophore production of E. anophelis is increased in the presence of oxidative stress.We further showed that hemoglobin facilitates the growth, hydrogen peroxide tolerance, cell attachment, and biofilm formation of E. anophelis NUHP1.Our study suggests that siderophore production and heme uptake pathways might play essential roles in stress response and virulence of the emerging pathogen E. anophelis.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, Nanyang Technological University, Singapore.

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Related in: MedlinePlus

Circular representation of sequence conservation between E. anophelis NUHP1 and 11 Elizabethkingia spp. strains for identify genome regions with high flexibility. Circles are numbered from 1 (outermost circle) to 14 (innermost circle). Circle 1: E. meningoseptica ATCC13253 (OSU). Circle 2: E. meningoseptica ATCC13253 (NITE). Circle 3: E. meningoseptica 502. Circle 4: E. anophelis R26. Circle 5: E. anophelis Ag1. Circle 6: E. anophelis NUH11. Circle 7: E. anophelis NUH6. Circle 8: E. anophelis NUH4. Circle 9: E. anophelis NUH1. Circle 10: E. anophelis NUHP3. Circle 11: E. anophelis NUHP2. Circle 12: GC skew (positive GC skew, green; negative GC skew, violet). Circle 13: GC content. Circle 14: scale of NUHP1 genome.
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evv101-F1: Circular representation of sequence conservation between E. anophelis NUHP1 and 11 Elizabethkingia spp. strains for identify genome regions with high flexibility. Circles are numbered from 1 (outermost circle) to 14 (innermost circle). Circle 1: E. meningoseptica ATCC13253 (OSU). Circle 2: E. meningoseptica ATCC13253 (NITE). Circle 3: E. meningoseptica 502. Circle 4: E. anophelis R26. Circle 5: E. anophelis Ag1. Circle 6: E. anophelis NUH11. Circle 7: E. anophelis NUH6. Circle 8: E. anophelis NUH4. Circle 9: E. anophelis NUH1. Circle 10: E. anophelis NUHP3. Circle 11: E. anophelis NUHP2. Circle 12: GC skew (positive GC skew, green; negative GC skew, violet). Circle 13: GC content. Circle 14: scale of NUHP1 genome.

Mentions: The genome of E. anophelis NUHP1 strain consists of a circular chromosome comprising 4,369,828 bp and average GC content of 35.62% (fig. 1). A total of 4,141 genes are predicted from the NUHP1 genome, where 4,074 of which are protein-coding genes and 67 of which are RNA-coding genes (52 tRNAs, 10 rRNAs, and 5 other RNAs). A total of 2,489 of the protein-coding genes can be assigned to a putative function with the remaining annotated as hypothetical proteins.Fig. 1.—


Complete Genome Sequence and Transcriptomic Analysis of the Novel Pathogen Elizabethkingia anophelis in Response to Oxidative Stress.

Li Y, Liu Y, Chew SC, Tay M, Salido MM, Teo J, Lauro FM, Givskov M, Yang L - Genome Biol Evol (2015)

Circular representation of sequence conservation between E. anophelis NUHP1 and 11 Elizabethkingia spp. strains for identify genome regions with high flexibility. Circles are numbered from 1 (outermost circle) to 14 (innermost circle). Circle 1: E. meningoseptica ATCC13253 (OSU). Circle 2: E. meningoseptica ATCC13253 (NITE). Circle 3: E. meningoseptica 502. Circle 4: E. anophelis R26. Circle 5: E. anophelis Ag1. Circle 6: E. anophelis NUH11. Circle 7: E. anophelis NUH6. Circle 8: E. anophelis NUH4. Circle 9: E. anophelis NUH1. Circle 10: E. anophelis NUHP3. Circle 11: E. anophelis NUHP2. Circle 12: GC skew (positive GC skew, green; negative GC skew, violet). Circle 13: GC content. Circle 14: scale of NUHP1 genome.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4494045&req=5

evv101-F1: Circular representation of sequence conservation between E. anophelis NUHP1 and 11 Elizabethkingia spp. strains for identify genome regions with high flexibility. Circles are numbered from 1 (outermost circle) to 14 (innermost circle). Circle 1: E. meningoseptica ATCC13253 (OSU). Circle 2: E. meningoseptica ATCC13253 (NITE). Circle 3: E. meningoseptica 502. Circle 4: E. anophelis R26. Circle 5: E. anophelis Ag1. Circle 6: E. anophelis NUH11. Circle 7: E. anophelis NUH6. Circle 8: E. anophelis NUH4. Circle 9: E. anophelis NUH1. Circle 10: E. anophelis NUHP3. Circle 11: E. anophelis NUHP2. Circle 12: GC skew (positive GC skew, green; negative GC skew, violet). Circle 13: GC content. Circle 14: scale of NUHP1 genome.
Mentions: The genome of E. anophelis NUHP1 strain consists of a circular chromosome comprising 4,369,828 bp and average GC content of 35.62% (fig. 1). A total of 4,141 genes are predicted from the NUHP1 genome, where 4,074 of which are protein-coding genes and 67 of which are RNA-coding genes (52 tRNAs, 10 rRNAs, and 5 other RNAs). A total of 2,489 of the protein-coding genes can be assigned to a putative function with the remaining annotated as hypothetical proteins.Fig. 1.—

Bottom Line: Chrome azurol sulfonate assay verified that siderophore production of E. anophelis is increased in the presence of oxidative stress.We further showed that hemoglobin facilitates the growth, hydrogen peroxide tolerance, cell attachment, and biofilm formation of E. anophelis NUHP1.Our study suggests that siderophore production and heme uptake pathways might play essential roles in stress response and virulence of the emerging pathogen E. anophelis.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, Nanyang Technological University, Singapore.

Show MeSH
Related in: MedlinePlus