Genome-Wide Analysis in Three Fusarium Pathogens Identifies Rapidly Evolving Chromosomes and Genes Associated with Pathogenicity.
Bottom Line: We found a two-speed genome structure both on the chromosome and gene group level.Members of two gene groups evolve rapidly, namely those that encode proteins with an N-terminal [SG]-P-C-[KR]-P sequence motif and proteins that are conserved predominantly in pathogens.Specifically, 29 F. graminearum genes are rapidly evolving, in planta induced and encode secreted proteins, strongly pointing toward effector function.
Affiliation: CSIRO Agriculture Flagship, Centre for Environment and Life Sciences, Perth, Western Australia, Australia firstname.lastname@example.org.Show MeSH
Related in: MedlinePlus
Mentions: We used statistical testing to determine the significance of differences in the number of genes under site-specific diversifying selection for the individual chromosomes in comparison to the remainder of the genome. On the dispensable F. oxysporum f. sp. lycopersici chromosomes, the percentage of genes that could be analyzed for site-specific diversifying selection is lower than for the core chromosomes (∼30–40% on dispensable chromosomes have a CODEML result compared with 69.7% genome-wide). Nevertheless, F. oxysporum f. sp. lycopersici has a significantly higher percentage of rapidly evolving genes on the dispensable chromosomes 3, 6, 14, and 15 as well as on chromosomes 2b (fig. 3B). Interestingly, the highest percentage of rapidly evolving genes for F. graminearum is found on chromosome 2 (fig. 3A). For F. verticillioides high percentages of rapidly evolving genes are located on chromosome 2, 7, 8, 6, 10, and 4 (fig. 3C).Fig. 3.—
Affiliation: CSIRO Agriculture Flagship, Centre for Environment and Life Sciences, Perth, Western Australia, Australia email@example.com.