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Soft UV nanoimprint lithography-designed highly sensitive substrates for SERS detection.

Cottat M, Lidgi-Guigui N, Tijunelyte I, Barbillon G, Hamouda F, Gogol P, Aassime A, Lourtioz JM, Bartenlian B, de la Chapelle ML - Nanoscale Res Lett (2014)

Bottom Line: Gold nanocylinders (GNCs) showed to be very sensitive and specific sensing surfaces.Indeed, we demonstrated that less than 4 ×10(6) avidin molecules were detected and contributed to the surface-enhanced Raman scattering (SERS) signal.Thus, the soft UV-NIL technique allows to obtain quickly very sensitive substrates for SERS biosensing on surfaces of 1 mm (2).

View Article: PubMed Central - PubMed

Affiliation: CSPBAT (UMR 7244), CNRS-Université Paris 13, 74 rue Marcel Cachin, 93017, Bobigny, France, maximilien.cottat@univ-paris13.fr.

ABSTRACT
We report on the use of soft UV nanoimprint lithography (UV-NIL) for the development of reproducible, millimeter-sized, and sensitive substrates for SERS detection. The used geometry for plasmonic nanostructures is the cylinder. Gold nanocylinders (GNCs) showed to be very sensitive and specific sensing surfaces. Indeed, we demonstrated that less than 4 ×10(6) avidin molecules were detected and contributed to the surface-enhanced Raman scattering (SERS) signal. Thus, the soft UV-NIL technique allows to obtain quickly very sensitive substrates for SERS biosensing on surfaces of 1 mm (2).

No MeSH data available.


Optical characterization: SPR and SERS.(a) SPR measurements showing the avidin adsorption onto functionalized gold layer (orange) and after four injections of BSA (green). Single arrows show the BSA injection, and double arrows avidin injections at 0.03, 0.1, 0.3, 1, 3, 10, 30, and 100 nM. (b) Raman spectra of biotin-NHS powder multiplied by 5 (black), biotin on GNCs (red), avidin + biotin on GNCs (green), and avidin solution multiplied by 20 (blue).
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Fig4: Optical characterization: SPR and SERS.(a) SPR measurements showing the avidin adsorption onto functionalized gold layer (orange) and after four injections of BSA (green). Single arrows show the BSA injection, and double arrows avidin injections at 0.03, 0.1, 0.3, 1, 3, 10, 30, and 100 nM. (b) Raman spectra of biotin-NHS powder multiplied by 5 (black), biotin on GNCs (red), avidin + biotin on GNCs (green), and avidin solution multiplied by 20 (blue).

Mentions: Avidin detection was performed, thanks to the GNC functionalization with biotin (to capture avidin) and DDT (to block the remaining clean gold surface). In order to validate the functionalization protocol, we have performed SPR measurements on bare gold surface prior to the SERS experiment. Figure 4a presents the result of this experiment. These experiments have been done on the same substrate (bare flat gold surfaces) with two different flow cells. Cysteamine, biotin, and DDT are very light molecules and they cannot be detected by this technique; this is why only the results concerning the adsorption of BSA and avidin are shown. The first curve (orange) shows the result of avidin adsorption on the functionalized gold surface. Increasing concentrations are successively injected followed by a rinse with water, and the result is seen as a curve with a staircase shape. The response units are sensitive to the refractive index of the gold surrounding layer, so that an increase in the response units translates as an increased adsorption of proteins. The first injections (i.e., 0 and 0.01 nM) do not correspond to any step because there are too few avidin molecules immobilized on the surface. For 0.03 nM, a low step is observed; however, this concentration is very low and is not sufficient to fulfill all the biotins present on the surface; 0.1 nM seems to be a good concentration to start the saturation of the biotinylated gold chip. In order to confirm the binding specificity of avidin to biotin, a second experiment was performed. A high concentration of BSA was injected and rinsed four times before the injection of avidin. Although BSA has been injected, the result shows a total recovery of the sensitivity in terms of avidin detection. The functionalization route is thus specific to avidin.Figure 4


Soft UV nanoimprint lithography-designed highly sensitive substrates for SERS detection.

Cottat M, Lidgi-Guigui N, Tijunelyte I, Barbillon G, Hamouda F, Gogol P, Aassime A, Lourtioz JM, Bartenlian B, de la Chapelle ML - Nanoscale Res Lett (2014)

Optical characterization: SPR and SERS.(a) SPR measurements showing the avidin adsorption onto functionalized gold layer (orange) and after four injections of BSA (green). Single arrows show the BSA injection, and double arrows avidin injections at 0.03, 0.1, 0.3, 1, 3, 10, 30, and 100 nM. (b) Raman spectra of biotin-NHS powder multiplied by 5 (black), biotin on GNCs (red), avidin + biotin on GNCs (green), and avidin solution multiplied by 20 (blue).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494019&req=5

Fig4: Optical characterization: SPR and SERS.(a) SPR measurements showing the avidin adsorption onto functionalized gold layer (orange) and after four injections of BSA (green). Single arrows show the BSA injection, and double arrows avidin injections at 0.03, 0.1, 0.3, 1, 3, 10, 30, and 100 nM. (b) Raman spectra of biotin-NHS powder multiplied by 5 (black), biotin on GNCs (red), avidin + biotin on GNCs (green), and avidin solution multiplied by 20 (blue).
Mentions: Avidin detection was performed, thanks to the GNC functionalization with biotin (to capture avidin) and DDT (to block the remaining clean gold surface). In order to validate the functionalization protocol, we have performed SPR measurements on bare gold surface prior to the SERS experiment. Figure 4a presents the result of this experiment. These experiments have been done on the same substrate (bare flat gold surfaces) with two different flow cells. Cysteamine, biotin, and DDT are very light molecules and they cannot be detected by this technique; this is why only the results concerning the adsorption of BSA and avidin are shown. The first curve (orange) shows the result of avidin adsorption on the functionalized gold surface. Increasing concentrations are successively injected followed by a rinse with water, and the result is seen as a curve with a staircase shape. The response units are sensitive to the refractive index of the gold surrounding layer, so that an increase in the response units translates as an increased adsorption of proteins. The first injections (i.e., 0 and 0.01 nM) do not correspond to any step because there are too few avidin molecules immobilized on the surface. For 0.03 nM, a low step is observed; however, this concentration is very low and is not sufficient to fulfill all the biotins present on the surface; 0.1 nM seems to be a good concentration to start the saturation of the biotinylated gold chip. In order to confirm the binding specificity of avidin to biotin, a second experiment was performed. A high concentration of BSA was injected and rinsed four times before the injection of avidin. Although BSA has been injected, the result shows a total recovery of the sensitivity in terms of avidin detection. The functionalization route is thus specific to avidin.Figure 4

Bottom Line: Gold nanocylinders (GNCs) showed to be very sensitive and specific sensing surfaces.Indeed, we demonstrated that less than 4 ×10(6) avidin molecules were detected and contributed to the surface-enhanced Raman scattering (SERS) signal.Thus, the soft UV-NIL technique allows to obtain quickly very sensitive substrates for SERS biosensing on surfaces of 1 mm (2).

View Article: PubMed Central - PubMed

Affiliation: CSPBAT (UMR 7244), CNRS-Université Paris 13, 74 rue Marcel Cachin, 93017, Bobigny, France, maximilien.cottat@univ-paris13.fr.

ABSTRACT
We report on the use of soft UV nanoimprint lithography (UV-NIL) for the development of reproducible, millimeter-sized, and sensitive substrates for SERS detection. The used geometry for plasmonic nanostructures is the cylinder. Gold nanocylinders (GNCs) showed to be very sensitive and specific sensing surfaces. Indeed, we demonstrated that less than 4 ×10(6) avidin molecules were detected and contributed to the surface-enhanced Raman scattering (SERS) signal. Thus, the soft UV-NIL technique allows to obtain quickly very sensitive substrates for SERS biosensing on surfaces of 1 mm (2).

No MeSH data available.