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Soft UV nanoimprint lithography-designed highly sensitive substrates for SERS detection.

Cottat M, Lidgi-Guigui N, Tijunelyte I, Barbillon G, Hamouda F, Gogol P, Aassime A, Lourtioz JM, Bartenlian B, de la Chapelle ML - Nanoscale Res Lett (2014)

Bottom Line: Gold nanocylinders (GNCs) showed to be very sensitive and specific sensing surfaces.Indeed, we demonstrated that less than 4 ×10(6) avidin molecules were detected and contributed to the surface-enhanced Raman scattering (SERS) signal.Thus, the soft UV-NIL technique allows to obtain quickly very sensitive substrates for SERS biosensing on surfaces of 1 mm (2).

View Article: PubMed Central - PubMed

Affiliation: CSPBAT (UMR 7244), CNRS-Université Paris 13, 74 rue Marcel Cachin, 93017, Bobigny, France, maximilien.cottat@univ-paris13.fr.

ABSTRACT
We report on the use of soft UV nanoimprint lithography (UV-NIL) for the development of reproducible, millimeter-sized, and sensitive substrates for SERS detection. The used geometry for plasmonic nanostructures is the cylinder. Gold nanocylinders (GNCs) showed to be very sensitive and specific sensing surfaces. Indeed, we demonstrated that less than 4 ×10(6) avidin molecules were detected and contributed to the surface-enhanced Raman scattering (SERS) signal. Thus, the soft UV-NIL technique allows to obtain quickly very sensitive substrates for SERS biosensing on surfaces of 1 mm (2).

No MeSH data available.


Related in: MedlinePlus

Sketch of the functionalization process of different molecules.(1) Cysteamine, (2) biotin, (3) blocking with dodecanethiol, and (4) avidin.
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Fig2: Sketch of the functionalization process of different molecules.(1) Cysteamine, (2) biotin, (3) blocking with dodecanethiol, and (4) avidin.

Mentions: Cysteamine, biotin-NHS, dodecanethiol (DDT), avidin, and bovine serum albumin (BSA) were purchased from Sigma Aldrich (St. Louis, MO, USA). The same functionalization procedure was used for GNC (SERS detection) and flat gold film (SPR detection). Figure 2 represents a sketch of functionalization process, where gold surfaces were first functionalized with a cysteamine monolayer, by an overnight incubation in a 100 mM solution of cysteamine in water. The surfaces were then thoroughly rinsed with water. In a second step, the samples were dipped in a 10 mM solution of biotin-NHS in dymethylformamide (DMF) and left to react for 2 h. Afterwards, the sample was thoroughly rinsed first with DMF, then with water. In order to guarantee that no surface was left unfunctionalized, a blocking step was needed. To do that, the nanostructures were soaked in a pure DDT solution for 1 h and then rinsed with ethanol and water. In the SERS experiment, a solution of 1 mM avidin was used to demonstrate the sensing ability of the GNCs.Figure 2


Soft UV nanoimprint lithography-designed highly sensitive substrates for SERS detection.

Cottat M, Lidgi-Guigui N, Tijunelyte I, Barbillon G, Hamouda F, Gogol P, Aassime A, Lourtioz JM, Bartenlian B, de la Chapelle ML - Nanoscale Res Lett (2014)

Sketch of the functionalization process of different molecules.(1) Cysteamine, (2) biotin, (3) blocking with dodecanethiol, and (4) avidin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494019&req=5

Fig2: Sketch of the functionalization process of different molecules.(1) Cysteamine, (2) biotin, (3) blocking with dodecanethiol, and (4) avidin.
Mentions: Cysteamine, biotin-NHS, dodecanethiol (DDT), avidin, and bovine serum albumin (BSA) were purchased from Sigma Aldrich (St. Louis, MO, USA). The same functionalization procedure was used for GNC (SERS detection) and flat gold film (SPR detection). Figure 2 represents a sketch of functionalization process, where gold surfaces were first functionalized with a cysteamine monolayer, by an overnight incubation in a 100 mM solution of cysteamine in water. The surfaces were then thoroughly rinsed with water. In a second step, the samples were dipped in a 10 mM solution of biotin-NHS in dymethylformamide (DMF) and left to react for 2 h. Afterwards, the sample was thoroughly rinsed first with DMF, then with water. In order to guarantee that no surface was left unfunctionalized, a blocking step was needed. To do that, the nanostructures were soaked in a pure DDT solution for 1 h and then rinsed with ethanol and water. In the SERS experiment, a solution of 1 mM avidin was used to demonstrate the sensing ability of the GNCs.Figure 2

Bottom Line: Gold nanocylinders (GNCs) showed to be very sensitive and specific sensing surfaces.Indeed, we demonstrated that less than 4 ×10(6) avidin molecules were detected and contributed to the surface-enhanced Raman scattering (SERS) signal.Thus, the soft UV-NIL technique allows to obtain quickly very sensitive substrates for SERS biosensing on surfaces of 1 mm (2).

View Article: PubMed Central - PubMed

Affiliation: CSPBAT (UMR 7244), CNRS-Université Paris 13, 74 rue Marcel Cachin, 93017, Bobigny, France, maximilien.cottat@univ-paris13.fr.

ABSTRACT
We report on the use of soft UV nanoimprint lithography (UV-NIL) for the development of reproducible, millimeter-sized, and sensitive substrates for SERS detection. The used geometry for plasmonic nanostructures is the cylinder. Gold nanocylinders (GNCs) showed to be very sensitive and specific sensing surfaces. Indeed, we demonstrated that less than 4 ×10(6) avidin molecules were detected and contributed to the surface-enhanced Raman scattering (SERS) signal. Thus, the soft UV-NIL technique allows to obtain quickly very sensitive substrates for SERS biosensing on surfaces of 1 mm (2).

No MeSH data available.


Related in: MedlinePlus