Control of the pericentrosomal H2O2 level by peroxiredoxin I is critical for mitotic progression.
Bottom Line: The intracellular concentration of H2O2 increases as the cell cycle progresses.Whereas the centrosome is shielded from H2O2 through its association with the H2O2-eliminating enzyme peroxiredoxin I (PrxI) during interphase, the centrosome-associated PrxI is selectively inactivated through phosphorylation by Cdk1 during early mitosis, thereby exposing the centrosome to H2O2 and facilitating inactivation of centrosome-bound phosphatases.Dephosphorylation of PrxI by okadaic acid-sensitive phosphatases during late mitosis again shields the centrosome from H2O2 and thereby allows the reactivation of Cdk1-opposing phosphatases at the organelle.
Affiliation: Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, South Korea.Show MeSH
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Mentions: Using the antibodies to pCdh1, we investigated the effect of Cat-PACT expression on Ser40 phosphorylation in nocodazole-arrested cells. The amount of pCdh1 in HeLa cells expressing Cat-PACT was reduced by ∼30% compared with that in cells infected with the empty vector or expressing Cat-delPACT (Fig. 4, A and B), consistent with the notion that Cdh1 phosphorylation is highly influenced by pericentrosomal H2O2 level and that pCdh1 undergoes turnover at the centrosome (Raff et al., 2002).
Affiliation: Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, South Korea.