The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans.
Bottom Line: Previous work in Caenorhabditis elegans identified two ciliopathy-associated protein complexes or modules that direct assembly of transition zone Y-links.Co-inhibition of all three modules completely disrupted transition zone structure.Surprisingly, axoneme assembly was only mildly perturbed.
Affiliation: Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter (VBC), A-1030 Vienna, Austria.Show MeSH
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Mentions: While cilia assembly was largely unaffected in transition zone mutants, neuronal morphology was strongly perturbed. This was most clearly seen in phasmids, where dendrites collapsed almost entirely, with cilia found immediately adjacent to the cell body (Fig. 2 A). This phenomenon was previously reported in certain combinations of transition zone mutants (Williams et al., 2008, 2011). Loss of contact with the external environment rather than ciliogenesis defects could explain the observed lack of dye-filling, and dendrite lengths do correlate with dye-fill phenotypes (compare Figs. 3 C and S2 E). Complete dendrite collapse was not observed in amphids (Fig. S3, C and D). However, cilia frequently failed to extend into the channel formed by socket and sheath glia (Fig. S3, A and B).
Affiliation: Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter (VBC), A-1030 Vienna, Austria.