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Topically applied ceramide accumulates in skin glyphs.

Zhang Q, Flach CR, Mendelsohn R, Mao G, Pappas A, Mack MC, Walters RM, Southall MD - Clin Cosmet Investig Dermatol (2015)

Bottom Line: As a result, a very heterogeneous, sparse, spatial distribution of CERs in the SC was revealed.In contrast, oleic acid was found to be fairly homogeneously distributed throughout the SC and viable epidermis, albeit at lower concentrations in the latter.A more uniform, lateral distribution of CERs in the SC would likely be important for barrier efficacy or enhancement.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Rutgers University, Newark, NJ, USA.

ABSTRACT
Ceramides (CERs), structural components of the stratum corneum (SC), impart essential barrier properties to this thin outer layer of the epidermis. Variations in CER species within this layer have been linked to several skin diseases. A recent proliferation of CER-containing topical skin-care products warrants the elucidation of CER penetration profiles in both healthy and diseased skin. In the current study, the spatial distributions of CER concentration profiles, following topical application of two species of CER, were tracked using infrared imaging. Suspensions of single-chain perdeuterated sphingosine and phytosphingosine CER in oleic acid were applied, in separate experiments, to the surface of healthy intact ex vivo human skin using Franz diffusion cells. Following either a 24- or 48-hour incubation period at 34°C, infrared images were acquired from microtomed skin sections. Both CER species accumulated in glyph regions of the skin and penetrated into the SC, to a limited extent, only in these regions. The concentration profiles observed herein were independent of the CER species and incubation time utilized in the study. As a result, a very heterogeneous, sparse, spatial distribution of CERs in the SC was revealed. In contrast, oleic acid was found to be fairly homogeneously distributed throughout the SC and viable epidermis, albeit at lower concentrations in the latter. A more uniform, lateral distribution of CERs in the SC would likely be important for barrier efficacy or enhancement.

No MeSH data available.


Related in: MedlinePlus

(A) Typical single-pixel infrared spectra of N-palmitoyl-D-erythro-sphingosine (CER[NS]-d31) at different depths in skin. (B) The CD2 stretching region (2,234–2,064 cm−1) is highlighted at the same depths in A. This spectral region is baseline corrected.
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f1-ccid-8-329: (A) Typical single-pixel infrared spectra of N-palmitoyl-D-erythro-sphingosine (CER[NS]-d31) at different depths in skin. (B) The CD2 stretching region (2,234–2,064 cm−1) is highlighted at the same depths in A. This spectral region is baseline corrected.

Mentions: Typical single-pixel IR spectra of CER [NS]-d31 at different depths in skin are shown in Figure 1A with bands of interest marked. Figure 1B highlights the CD2 stretching region (2,234–2,064 cm−1) at the same depths. By integrating the area under the asymmetric CD2 stretching band and converting to CER [NS]-d31 concentration utilizing Beer’s law, we can quantitatively track the permeation of CER [NS]-d31 in skin. A high signal-to-noise ratio is evident in the spectra. The detection limit for CER [NS]-d31 is apparent in Figure 1B at a depth ~12.5 µm from the skin surface (third spectrum from top) and is 3.5 mM (~1.3 wt% of SC lipid phase) where the peak area is approximately two times the noise level.


Topically applied ceramide accumulates in skin glyphs.

Zhang Q, Flach CR, Mendelsohn R, Mao G, Pappas A, Mack MC, Walters RM, Southall MD - Clin Cosmet Investig Dermatol (2015)

(A) Typical single-pixel infrared spectra of N-palmitoyl-D-erythro-sphingosine (CER[NS]-d31) at different depths in skin. (B) The CD2 stretching region (2,234–2,064 cm−1) is highlighted at the same depths in A. This spectral region is baseline corrected.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493983&req=5

f1-ccid-8-329: (A) Typical single-pixel infrared spectra of N-palmitoyl-D-erythro-sphingosine (CER[NS]-d31) at different depths in skin. (B) The CD2 stretching region (2,234–2,064 cm−1) is highlighted at the same depths in A. This spectral region is baseline corrected.
Mentions: Typical single-pixel IR spectra of CER [NS]-d31 at different depths in skin are shown in Figure 1A with bands of interest marked. Figure 1B highlights the CD2 stretching region (2,234–2,064 cm−1) at the same depths. By integrating the area under the asymmetric CD2 stretching band and converting to CER [NS]-d31 concentration utilizing Beer’s law, we can quantitatively track the permeation of CER [NS]-d31 in skin. A high signal-to-noise ratio is evident in the spectra. The detection limit for CER [NS]-d31 is apparent in Figure 1B at a depth ~12.5 µm from the skin surface (third spectrum from top) and is 3.5 mM (~1.3 wt% of SC lipid phase) where the peak area is approximately two times the noise level.

Bottom Line: As a result, a very heterogeneous, sparse, spatial distribution of CERs in the SC was revealed.In contrast, oleic acid was found to be fairly homogeneously distributed throughout the SC and viable epidermis, albeit at lower concentrations in the latter.A more uniform, lateral distribution of CERs in the SC would likely be important for barrier efficacy or enhancement.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Rutgers University, Newark, NJ, USA.

ABSTRACT
Ceramides (CERs), structural components of the stratum corneum (SC), impart essential barrier properties to this thin outer layer of the epidermis. Variations in CER species within this layer have been linked to several skin diseases. A recent proliferation of CER-containing topical skin-care products warrants the elucidation of CER penetration profiles in both healthy and diseased skin. In the current study, the spatial distributions of CER concentration profiles, following topical application of two species of CER, were tracked using infrared imaging. Suspensions of single-chain perdeuterated sphingosine and phytosphingosine CER in oleic acid were applied, in separate experiments, to the surface of healthy intact ex vivo human skin using Franz diffusion cells. Following either a 24- or 48-hour incubation period at 34°C, infrared images were acquired from microtomed skin sections. Both CER species accumulated in glyph regions of the skin and penetrated into the SC, to a limited extent, only in these regions. The concentration profiles observed herein were independent of the CER species and incubation time utilized in the study. As a result, a very heterogeneous, sparse, spatial distribution of CERs in the SC was revealed. In contrast, oleic acid was found to be fairly homogeneously distributed throughout the SC and viable epidermis, albeit at lower concentrations in the latter. A more uniform, lateral distribution of CERs in the SC would likely be important for barrier efficacy or enhancement.

No MeSH data available.


Related in: MedlinePlus