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Systemic signs of neutrophil mobilization during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease.

Andelid K, Andersson A, Yoshihara S, Åhrén C, Jirholt P, Ekberg-Jansson A, Lindén A - Int J Chron Obstruct Pulmon Dis (2015)

Bottom Line: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers.However, MPO and NE protein and mRNA displayed positive correlations.In this condition, MPO and NE may share a cellular origin, but its location remains uncertain.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.

ABSTRACT

Background: It is still unclear whether signs of neutrophil mobilization in the blood of patients with chronic obstructive pulmonary disease represent true systemic events and how these relate to bacterial colonization in the airways. In this study, we evaluated these issues during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease and chronic bronchitis (OPD-CB).

Methods: Over a period of 60 weeks for each subject, blood samples were repeatedly collected from 60 smokers with OPD-CB during clinically stable periods, as well as during and after exacerbations. Myeloperoxidase (MPO) and neutrophil elastase (NE) protein and mRNA, growth of bacteria in sputum, and clinical parameters were analyzed. Ten asymptomatic smokers and ten never-smokers were included as controls.

Results: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers. During exacerbations, neutrophil and MPO protein concentrations were further increased in smokers with OPD-CB, without a detectable increase in the corresponding mRNA during exacerbations. However, MPO and NE protein and mRNA displayed positive correlations. During exacerbations, only increased neutrophil concentrations were associated with growth of bacteria in sputum. Among patients with low transcutaneous oxygen saturation during exacerbations, PaO2 (partial oxygen pressure) correlated with concentrations of MPO and NE protein and neutrophils in a negative manner.

Conclusion: There are signs of systemic neutrophil mobilization during clinically stable periods and even more so during exacerbations in chronic obstructive pulmonary disease. In this condition, MPO and NE may share a cellular origin, but its location remains uncertain. Factors other than local bacteria, including hypoxemia, may be important for driving systemic signs of neutrophil mobilization.

No MeSH data available.


Related in: MedlinePlus

Correlations between oxygen saturation and signs of systemic inflammation in smokers with obstructive pulmonary disease and chronic bronchitis (n=18) who displayed transcutaneous hypoxia during exacerbations.Notes: (A) PaO2 and blood neutrophil concentrations (ρ=0.63, P<0.01, Spearman rank correlation). (B) PaO2 and blood concentrations of NE protein (ρ=0.56, P<0.05, Spearman rank correlation). (C) PaO2 and blood concentrations of MPO protein (ρ=0.5, P<0.05, Spearman rank correlation).Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase; PaO2, partial oxygen pressure.
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f6-copd-10-1253: Correlations between oxygen saturation and signs of systemic inflammation in smokers with obstructive pulmonary disease and chronic bronchitis (n=18) who displayed transcutaneous hypoxia during exacerbations.Notes: (A) PaO2 and blood neutrophil concentrations (ρ=0.63, P<0.01, Spearman rank correlation). (B) PaO2 and blood concentrations of NE protein (ρ=0.56, P<0.05, Spearman rank correlation). (C) PaO2 and blood concentrations of MPO protein (ρ=0.5, P<0.05, Spearman rank correlation).Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase; PaO2, partial oxygen pressure.

Mentions: In 18 smokers with OPD-CB who developed exacerbations, the transcutaneous oxygen saturation was below 94%, but none of these individuals required mechanical ventilation. In this respect, the clinical characteristics did not markedly differ for mild or severe OPD-CB (Table 2). Arterial blood gas was analyzed in all smokers with low transcutaneous oxygen saturation in accordance with clinical guidelines. We then detected statistically significant negative correlations between PaO2 (partial oxygen pressure) and concentrations of neutrophils and NE and MPO protein, respectively (Figure 6A–C). However, all smokers with OPD-CB had normal PaCO2 during exacerbations.


Systemic signs of neutrophil mobilization during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease.

Andelid K, Andersson A, Yoshihara S, Åhrén C, Jirholt P, Ekberg-Jansson A, Lindén A - Int J Chron Obstruct Pulmon Dis (2015)

Correlations between oxygen saturation and signs of systemic inflammation in smokers with obstructive pulmonary disease and chronic bronchitis (n=18) who displayed transcutaneous hypoxia during exacerbations.Notes: (A) PaO2 and blood neutrophil concentrations (ρ=0.63, P<0.01, Spearman rank correlation). (B) PaO2 and blood concentrations of NE protein (ρ=0.56, P<0.05, Spearman rank correlation). (C) PaO2 and blood concentrations of MPO protein (ρ=0.5, P<0.05, Spearman rank correlation).Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase; PaO2, partial oxygen pressure.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493974&req=5

f6-copd-10-1253: Correlations between oxygen saturation and signs of systemic inflammation in smokers with obstructive pulmonary disease and chronic bronchitis (n=18) who displayed transcutaneous hypoxia during exacerbations.Notes: (A) PaO2 and blood neutrophil concentrations (ρ=0.63, P<0.01, Spearman rank correlation). (B) PaO2 and blood concentrations of NE protein (ρ=0.56, P<0.05, Spearman rank correlation). (C) PaO2 and blood concentrations of MPO protein (ρ=0.5, P<0.05, Spearman rank correlation).Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase; PaO2, partial oxygen pressure.
Mentions: In 18 smokers with OPD-CB who developed exacerbations, the transcutaneous oxygen saturation was below 94%, but none of these individuals required mechanical ventilation. In this respect, the clinical characteristics did not markedly differ for mild or severe OPD-CB (Table 2). Arterial blood gas was analyzed in all smokers with low transcutaneous oxygen saturation in accordance with clinical guidelines. We then detected statistically significant negative correlations between PaO2 (partial oxygen pressure) and concentrations of neutrophils and NE and MPO protein, respectively (Figure 6A–C). However, all smokers with OPD-CB had normal PaCO2 during exacerbations.

Bottom Line: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers.However, MPO and NE protein and mRNA displayed positive correlations.In this condition, MPO and NE may share a cellular origin, but its location remains uncertain.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.

ABSTRACT

Background: It is still unclear whether signs of neutrophil mobilization in the blood of patients with chronic obstructive pulmonary disease represent true systemic events and how these relate to bacterial colonization in the airways. In this study, we evaluated these issues during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease and chronic bronchitis (OPD-CB).

Methods: Over a period of 60 weeks for each subject, blood samples were repeatedly collected from 60 smokers with OPD-CB during clinically stable periods, as well as during and after exacerbations. Myeloperoxidase (MPO) and neutrophil elastase (NE) protein and mRNA, growth of bacteria in sputum, and clinical parameters were analyzed. Ten asymptomatic smokers and ten never-smokers were included as controls.

Results: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers. During exacerbations, neutrophil and MPO protein concentrations were further increased in smokers with OPD-CB, without a detectable increase in the corresponding mRNA during exacerbations. However, MPO and NE protein and mRNA displayed positive correlations. During exacerbations, only increased neutrophil concentrations were associated with growth of bacteria in sputum. Among patients with low transcutaneous oxygen saturation during exacerbations, PaO2 (partial oxygen pressure) correlated with concentrations of MPO and NE protein and neutrophils in a negative manner.

Conclusion: There are signs of systemic neutrophil mobilization during clinically stable periods and even more so during exacerbations in chronic obstructive pulmonary disease. In this condition, MPO and NE may share a cellular origin, but its location remains uncertain. Factors other than local bacteria, including hypoxemia, may be important for driving systemic signs of neutrophil mobilization.

No MeSH data available.


Related in: MedlinePlus