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Systemic signs of neutrophil mobilization during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease.

Andelid K, Andersson A, Yoshihara S, Åhrén C, Jirholt P, Ekberg-Jansson A, Lindén A - Int J Chron Obstruct Pulmon Dis (2015)

Bottom Line: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers.However, MPO and NE protein and mRNA displayed positive correlations.In this condition, MPO and NE may share a cellular origin, but its location remains uncertain.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.

ABSTRACT

Background: It is still unclear whether signs of neutrophil mobilization in the blood of patients with chronic obstructive pulmonary disease represent true systemic events and how these relate to bacterial colonization in the airways. In this study, we evaluated these issues during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease and chronic bronchitis (OPD-CB).

Methods: Over a period of 60 weeks for each subject, blood samples were repeatedly collected from 60 smokers with OPD-CB during clinically stable periods, as well as during and after exacerbations. Myeloperoxidase (MPO) and neutrophil elastase (NE) protein and mRNA, growth of bacteria in sputum, and clinical parameters were analyzed. Ten asymptomatic smokers and ten never-smokers were included as controls.

Results: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers. During exacerbations, neutrophil and MPO protein concentrations were further increased in smokers with OPD-CB, without a detectable increase in the corresponding mRNA during exacerbations. However, MPO and NE protein and mRNA displayed positive correlations. During exacerbations, only increased neutrophil concentrations were associated with growth of bacteria in sputum. Among patients with low transcutaneous oxygen saturation during exacerbations, PaO2 (partial oxygen pressure) correlated with concentrations of MPO and NE protein and neutrophils in a negative manner.

Conclusion: There are signs of systemic neutrophil mobilization during clinically stable periods and even more so during exacerbations in chronic obstructive pulmonary disease. In this condition, MPO and NE may share a cellular origin, but its location remains uncertain. Factors other than local bacteria, including hypoxemia, may be important for driving systemic signs of neutrophil mobilization.

No MeSH data available.


Related in: MedlinePlus

Correlations between (A) blood concentrations of MPO and NE protein (ρ=0.4, P<0.05, Spearman rank correlation) and (B) levels of MPO and NE mRNA in blood leukocytes (ρ=0.89, P<0.001, Spearman rank correlation) from smokers with obstructive pulmonary disease and chronic bronchitis (n=34) during exacerbations. The data are presented as individual values.Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase.
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f5-copd-10-1253: Correlations between (A) blood concentrations of MPO and NE protein (ρ=0.4, P<0.05, Spearman rank correlation) and (B) levels of MPO and NE mRNA in blood leukocytes (ρ=0.89, P<0.001, Spearman rank correlation) from smokers with obstructive pulmonary disease and chronic bronchitis (n=34) during exacerbations. The data are presented as individual values.Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase.

Mentions: Smokers with OPD-CB showed a clear but modest increase in leukocyte and neutrophil concentrations during exacerbations (Table 1). Likewise, the MPO protein concentration were increased in a clear but modest manner, and both neutrophils and MPO (Figure 4) tended to normalize at the time of the first regular visit after exacerbation, which was up to 15 weeks later. Moreover, NE protein tended to be increased during exacerbations but not in a statistically significant manner, whereas CRP was clearly increased during exacerbations (Table 1). Importantly, there was a correlation between concentrations of MPO and those of NE protein in smokers with OPD-CB during exacerbations (Figure 5A). In addition, smokers with OPD-CB showed a clear increase in CRP during exacerbations (Table 2). However, we failed to detect any correlation between values at the time of inclusion and those during exacerbations for MPO protein (ρ=0.056, P>0.05, Spearman rank correlation; n=35) or NE protein (ρ=0.053, P>0.05, Spearman rank correlation; n=36) and neutrophil (ρ=0.009, P>0.05, Spearman rank correlation; n=37) concentrations, respectively; this was also true for CRP concentrations (ρ=0.16, P>0.05, Spearman rank correlation; n=37; data not shown). In addition to this, we did not find any predictive value of MPO or NE with regard to exacerbations (data not shown).


Systemic signs of neutrophil mobilization during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease.

Andelid K, Andersson A, Yoshihara S, Åhrén C, Jirholt P, Ekberg-Jansson A, Lindén A - Int J Chron Obstruct Pulmon Dis (2015)

Correlations between (A) blood concentrations of MPO and NE protein (ρ=0.4, P<0.05, Spearman rank correlation) and (B) levels of MPO and NE mRNA in blood leukocytes (ρ=0.89, P<0.001, Spearman rank correlation) from smokers with obstructive pulmonary disease and chronic bronchitis (n=34) during exacerbations. The data are presented as individual values.Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493974&req=5

f5-copd-10-1253: Correlations between (A) blood concentrations of MPO and NE protein (ρ=0.4, P<0.05, Spearman rank correlation) and (B) levels of MPO and NE mRNA in blood leukocytes (ρ=0.89, P<0.001, Spearman rank correlation) from smokers with obstructive pulmonary disease and chronic bronchitis (n=34) during exacerbations. The data are presented as individual values.Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase.
Mentions: Smokers with OPD-CB showed a clear but modest increase in leukocyte and neutrophil concentrations during exacerbations (Table 1). Likewise, the MPO protein concentration were increased in a clear but modest manner, and both neutrophils and MPO (Figure 4) tended to normalize at the time of the first regular visit after exacerbation, which was up to 15 weeks later. Moreover, NE protein tended to be increased during exacerbations but not in a statistically significant manner, whereas CRP was clearly increased during exacerbations (Table 1). Importantly, there was a correlation between concentrations of MPO and those of NE protein in smokers with OPD-CB during exacerbations (Figure 5A). In addition, smokers with OPD-CB showed a clear increase in CRP during exacerbations (Table 2). However, we failed to detect any correlation between values at the time of inclusion and those during exacerbations for MPO protein (ρ=0.056, P>0.05, Spearman rank correlation; n=35) or NE protein (ρ=0.053, P>0.05, Spearman rank correlation; n=36) and neutrophil (ρ=0.009, P>0.05, Spearman rank correlation; n=37) concentrations, respectively; this was also true for CRP concentrations (ρ=0.16, P>0.05, Spearman rank correlation; n=37; data not shown). In addition to this, we did not find any predictive value of MPO or NE with regard to exacerbations (data not shown).

Bottom Line: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers.However, MPO and NE protein and mRNA displayed positive correlations.In this condition, MPO and NE may share a cellular origin, but its location remains uncertain.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.

ABSTRACT

Background: It is still unclear whether signs of neutrophil mobilization in the blood of patients with chronic obstructive pulmonary disease represent true systemic events and how these relate to bacterial colonization in the airways. In this study, we evaluated these issues during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease and chronic bronchitis (OPD-CB).

Methods: Over a period of 60 weeks for each subject, blood samples were repeatedly collected from 60 smokers with OPD-CB during clinically stable periods, as well as during and after exacerbations. Myeloperoxidase (MPO) and neutrophil elastase (NE) protein and mRNA, growth of bacteria in sputum, and clinical parameters were analyzed. Ten asymptomatic smokers and ten never-smokers were included as controls.

Results: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers. During exacerbations, neutrophil and MPO protein concentrations were further increased in smokers with OPD-CB, without a detectable increase in the corresponding mRNA during exacerbations. However, MPO and NE protein and mRNA displayed positive correlations. During exacerbations, only increased neutrophil concentrations were associated with growth of bacteria in sputum. Among patients with low transcutaneous oxygen saturation during exacerbations, PaO2 (partial oxygen pressure) correlated with concentrations of MPO and NE protein and neutrophils in a negative manner.

Conclusion: There are signs of systemic neutrophil mobilization during clinically stable periods and even more so during exacerbations in chronic obstructive pulmonary disease. In this condition, MPO and NE may share a cellular origin, but its location remains uncertain. Factors other than local bacteria, including hypoxemia, may be important for driving systemic signs of neutrophil mobilization.

No MeSH data available.


Related in: MedlinePlus