Limits...
Systemic signs of neutrophil mobilization during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease.

Andelid K, Andersson A, Yoshihara S, Åhrén C, Jirholt P, Ekberg-Jansson A, Lindén A - Int J Chron Obstruct Pulmon Dis (2015)

Bottom Line: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers.However, MPO and NE protein and mRNA displayed positive correlations.In this condition, MPO and NE may share a cellular origin, but its location remains uncertain.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.

ABSTRACT

Background: It is still unclear whether signs of neutrophil mobilization in the blood of patients with chronic obstructive pulmonary disease represent true systemic events and how these relate to bacterial colonization in the airways. In this study, we evaluated these issues during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease and chronic bronchitis (OPD-CB).

Methods: Over a period of 60 weeks for each subject, blood samples were repeatedly collected from 60 smokers with OPD-CB during clinically stable periods, as well as during and after exacerbations. Myeloperoxidase (MPO) and neutrophil elastase (NE) protein and mRNA, growth of bacteria in sputum, and clinical parameters were analyzed. Ten asymptomatic smokers and ten never-smokers were included as controls.

Results: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers. During exacerbations, neutrophil and MPO protein concentrations were further increased in smokers with OPD-CB, without a detectable increase in the corresponding mRNA during exacerbations. However, MPO and NE protein and mRNA displayed positive correlations. During exacerbations, only increased neutrophil concentrations were associated with growth of bacteria in sputum. Among patients with low transcutaneous oxygen saturation during exacerbations, PaO2 (partial oxygen pressure) correlated with concentrations of MPO and NE protein and neutrophils in a negative manner.

Conclusion: There are signs of systemic neutrophil mobilization during clinically stable periods and even more so during exacerbations in chronic obstructive pulmonary disease. In this condition, MPO and NE may share a cellular origin, but its location remains uncertain. Factors other than local bacteria, including hypoxemia, may be important for driving systemic signs of neutrophil mobilization.

No MeSH data available.


Related in: MedlinePlus

Correlations between (A) blood concentrations of MPO protein and neutrophils (ρ=0.38, P<0.05, Spearman rank correlation) and (B) blood concentrations of NE protein and neutrophils (ρ=0.38, P<0.05, Spearman rank correlation) of smokers with obstructive pulmonary disease and chronic bronchitis (n=34) during a clinically stable period at the time of inclusion.Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493974&req=5

f2-copd-10-1253: Correlations between (A) blood concentrations of MPO protein and neutrophils (ρ=0.38, P<0.05, Spearman rank correlation) and (B) blood concentrations of NE protein and neutrophils (ρ=0.38, P<0.05, Spearman rank correlation) of smokers with obstructive pulmonary disease and chronic bronchitis (n=34) during a clinically stable period at the time of inclusion.Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase.

Mentions: At the time of inclusion, neutrophil concentrations were clearly higher in smokers with OPD-CB and the AS group when compared with the NS group (Figure 1A), even though the differences were modest. However, there was no statistically significant difference in MPO protein concentrations between smokers with OPD-CB and the AS or NS groups; only weak trends toward an increase in smokers with OPD-CB were observed (Figure 1B). In contrast, NE protein concentrations were clearly higher in smokers with OPD-CB and in the AS group when compared with the NS group (Figure 1C). CRP concentrations were clearly higher in smokers with OPD-CB than in either of the control groups, ie, AS and NS (Figure 1D). In addition, there was a moderate positive correlation between MPO and NE protein concentrations and neutrophil concentrations in smokers with OPD-CB (Figure 2A and B). We were unable to identify a statistically significant correlation between CRP and NE (ρ=0.043, P>0.05, Spearman rank correlation; n=58) or between CRP and MPO (ρ=0.16, P>0.05, Spearman rank correlation; n=59) during clinically stable periods in smokers with OPD-CB at the time of inclusion (data not shown).


Systemic signs of neutrophil mobilization during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease.

Andelid K, Andersson A, Yoshihara S, Åhrén C, Jirholt P, Ekberg-Jansson A, Lindén A - Int J Chron Obstruct Pulmon Dis (2015)

Correlations between (A) blood concentrations of MPO protein and neutrophils (ρ=0.38, P<0.05, Spearman rank correlation) and (B) blood concentrations of NE protein and neutrophils (ρ=0.38, P<0.05, Spearman rank correlation) of smokers with obstructive pulmonary disease and chronic bronchitis (n=34) during a clinically stable period at the time of inclusion.Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493974&req=5

f2-copd-10-1253: Correlations between (A) blood concentrations of MPO protein and neutrophils (ρ=0.38, P<0.05, Spearman rank correlation) and (B) blood concentrations of NE protein and neutrophils (ρ=0.38, P<0.05, Spearman rank correlation) of smokers with obstructive pulmonary disease and chronic bronchitis (n=34) during a clinically stable period at the time of inclusion.Abbreviations: MPO, myeloperoxidase; NE, neutrophil elastase.
Mentions: At the time of inclusion, neutrophil concentrations were clearly higher in smokers with OPD-CB and the AS group when compared with the NS group (Figure 1A), even though the differences were modest. However, there was no statistically significant difference in MPO protein concentrations between smokers with OPD-CB and the AS or NS groups; only weak trends toward an increase in smokers with OPD-CB were observed (Figure 1B). In contrast, NE protein concentrations were clearly higher in smokers with OPD-CB and in the AS group when compared with the NS group (Figure 1C). CRP concentrations were clearly higher in smokers with OPD-CB than in either of the control groups, ie, AS and NS (Figure 1D). In addition, there was a moderate positive correlation between MPO and NE protein concentrations and neutrophil concentrations in smokers with OPD-CB (Figure 2A and B). We were unable to identify a statistically significant correlation between CRP and NE (ρ=0.043, P>0.05, Spearman rank correlation; n=58) or between CRP and MPO (ρ=0.16, P>0.05, Spearman rank correlation; n=59) during clinically stable periods in smokers with OPD-CB at the time of inclusion (data not shown).

Bottom Line: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers.However, MPO and NE protein and mRNA displayed positive correlations.In this condition, MPO and NE may share a cellular origin, but its location remains uncertain.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.

ABSTRACT

Background: It is still unclear whether signs of neutrophil mobilization in the blood of patients with chronic obstructive pulmonary disease represent true systemic events and how these relate to bacterial colonization in the airways. In this study, we evaluated these issues during clinically stable periods and during exacerbations in smokers with obstructive pulmonary disease and chronic bronchitis (OPD-CB).

Methods: Over a period of 60 weeks for each subject, blood samples were repeatedly collected from 60 smokers with OPD-CB during clinically stable periods, as well as during and after exacerbations. Myeloperoxidase (MPO) and neutrophil elastase (NE) protein and mRNA, growth of bacteria in sputum, and clinical parameters were analyzed. Ten asymptomatic smokers and ten never-smokers were included as controls.

Results: We found that, during clinically stable periods, neutrophil and NE protein concentrations were increased in smokers with OPD-CB and in the asymptomatic smokers when compared with never-smokers. During exacerbations, neutrophil and MPO protein concentrations were further increased in smokers with OPD-CB, without a detectable increase in the corresponding mRNA during exacerbations. However, MPO and NE protein and mRNA displayed positive correlations. During exacerbations, only increased neutrophil concentrations were associated with growth of bacteria in sputum. Among patients with low transcutaneous oxygen saturation during exacerbations, PaO2 (partial oxygen pressure) correlated with concentrations of MPO and NE protein and neutrophils in a negative manner.

Conclusion: There are signs of systemic neutrophil mobilization during clinically stable periods and even more so during exacerbations in chronic obstructive pulmonary disease. In this condition, MPO and NE may share a cellular origin, but its location remains uncertain. Factors other than local bacteria, including hypoxemia, may be important for driving systemic signs of neutrophil mobilization.

No MeSH data available.


Related in: MedlinePlus