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Positive contrast high-resolution 3D-cine imaging of the cardiovascular system in small animals using a UTE sequence and iron nanoparticles at 4.7, 7 and 9.4 T.

Trotier AJ, Lefrançois W, Van Renterghem K, Franconi JM, Thiaudière E, Miraux S - J Cardiovasc Magn Reson (2015)

Bottom Line: UTE imaging generated positive contrast and higher SNR and CNR whatever the magnetic field and the USPIO concentration used compared to pre-contrast images.We have demonstrated that by combining the injection of iron nanoparticles with 3D-cine UTE sequences, it was possible to generate a strong positive contrast between blood and surrounding tissues.This approach might be useful to measure the functional cardiac parameters or to assess anatomical modifications to the blood vessels in cardio-vascular disease models.

View Article: PubMed Central - PubMed

Affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 CNRS/Université de Bordeaux, 146 rue Léo Saignat, Cedex 33076, Bordeaux, France. trotier@rmsb.u-bordeaux2.fr.

ABSTRACT

Background: To show that 3D sequences with ultra-short echo times (UTEs) can generate a positive contrast whatever the magnetic field (4.7, 7 or 9.4 T) and whatever Ultra Small Particles of Iron Oxide (USPIO) concentration injected and to use it for 3D time-resolved imaging of the murine cardiovascular system with high spatial and temporal resolutions.

Methods: Three different concentrations (50, 200 and 500 μmol Fe/kg) of USPIO were injected in mice and static images of the middle part of the animals were acquired at 4.7, 7 and 9.4 T pre and post-contrast with UTE (TE/TR = 0.05/4.5 ms) sequences. Signal-to-Noise Ratio (SNR) and Contrast-to-Noise Ratio (CNR) of blood and static tissus were evaluated before and after contrast agent injection. 3D-cine images (TE/TR = 0.05/3.5 ms, scan time < 12 min) at 156 μm isotropic resolution of the mouse cardiopulmonary system were acquired prospectively with the UTE sequence for the three magnetic fields and with an USPIO dose of 200 μmol Fe/kg. SNR, CNR and signal homogeneity of blood were measured. High spatial (104 μm) or temporal (3.5 ms) resolution 3D-cine imaging (scan time < 35 min) isotropic resolution were also performed at 7 T with a new sequence encoding scheme.

Results: UTE imaging generated positive contrast and higher SNR and CNR whatever the magnetic field and the USPIO concentration used compared to pre-contrast images. Time-resolved 3D acquisition enables high blood SNR (66.6 ± 4.5 at 7 T) and CNR (33.2 ± 4.2 at 7 T) without flow or motion artefact. Coronary arteries and aortic valve were visible on images acquired at 104 μm resolution.

Conclusions: We have demonstrated that by combining the injection of iron nanoparticles with 3D-cine UTE sequences, it was possible to generate a strong positive contrast between blood and surrounding tissues. These properties were exploited to produce images of the cardiovascular system in small animals at high magnetic fields with a high spatial and temporal resolution. This approach might be useful to measure the functional cardiac parameters or to assess anatomical modifications to the blood vessels in cardio-vascular disease models.

No MeSH data available.


Related in: MedlinePlus

Contrast-to-noise ratio between blood in the aortic arch and muscle, obtained with UTE sequences before and after injection of USPIO at 4.7, 7 and 9.4 T and for different injection doses (200 and 500 μmol Fe/kg). p values lower than 0.05 are indicated by an asterisk
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Fig3: Contrast-to-noise ratio between blood in the aortic arch and muscle, obtained with UTE sequences before and after injection of USPIO at 4.7, 7 and 9.4 T and for different injection doses (200 and 500 μmol Fe/kg). p values lower than 0.05 are indicated by an asterisk

Mentions: The same experiments were performed at 4.7 T, 7.0 T and 9.4 T, and the signal-to-noise ratio was measured for blood (aortic arch, jugular vein) and for the muscles. The blood-to-muscle contrast-to-noise ratios under the different conditions are indicated in Fig. 3.Fig. 3


Positive contrast high-resolution 3D-cine imaging of the cardiovascular system in small animals using a UTE sequence and iron nanoparticles at 4.7, 7 and 9.4 T.

Trotier AJ, Lefrançois W, Van Renterghem K, Franconi JM, Thiaudière E, Miraux S - J Cardiovasc Magn Reson (2015)

Contrast-to-noise ratio between blood in the aortic arch and muscle, obtained with UTE sequences before and after injection of USPIO at 4.7, 7 and 9.4 T and for different injection doses (200 and 500 μmol Fe/kg). p values lower than 0.05 are indicated by an asterisk
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493959&req=5

Fig3: Contrast-to-noise ratio between blood in the aortic arch and muscle, obtained with UTE sequences before and after injection of USPIO at 4.7, 7 and 9.4 T and for different injection doses (200 and 500 μmol Fe/kg). p values lower than 0.05 are indicated by an asterisk
Mentions: The same experiments were performed at 4.7 T, 7.0 T and 9.4 T, and the signal-to-noise ratio was measured for blood (aortic arch, jugular vein) and for the muscles. The blood-to-muscle contrast-to-noise ratios under the different conditions are indicated in Fig. 3.Fig. 3

Bottom Line: UTE imaging generated positive contrast and higher SNR and CNR whatever the magnetic field and the USPIO concentration used compared to pre-contrast images.We have demonstrated that by combining the injection of iron nanoparticles with 3D-cine UTE sequences, it was possible to generate a strong positive contrast between blood and surrounding tissues.This approach might be useful to measure the functional cardiac parameters or to assess anatomical modifications to the blood vessels in cardio-vascular disease models.

View Article: PubMed Central - PubMed

Affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 CNRS/Université de Bordeaux, 146 rue Léo Saignat, Cedex 33076, Bordeaux, France. trotier@rmsb.u-bordeaux2.fr.

ABSTRACT

Background: To show that 3D sequences with ultra-short echo times (UTEs) can generate a positive contrast whatever the magnetic field (4.7, 7 or 9.4 T) and whatever Ultra Small Particles of Iron Oxide (USPIO) concentration injected and to use it for 3D time-resolved imaging of the murine cardiovascular system with high spatial and temporal resolutions.

Methods: Three different concentrations (50, 200 and 500 μmol Fe/kg) of USPIO were injected in mice and static images of the middle part of the animals were acquired at 4.7, 7 and 9.4 T pre and post-contrast with UTE (TE/TR = 0.05/4.5 ms) sequences. Signal-to-Noise Ratio (SNR) and Contrast-to-Noise Ratio (CNR) of blood and static tissus were evaluated before and after contrast agent injection. 3D-cine images (TE/TR = 0.05/3.5 ms, scan time < 12 min) at 156 μm isotropic resolution of the mouse cardiopulmonary system were acquired prospectively with the UTE sequence for the three magnetic fields and with an USPIO dose of 200 μmol Fe/kg. SNR, CNR and signal homogeneity of blood were measured. High spatial (104 μm) or temporal (3.5 ms) resolution 3D-cine imaging (scan time < 35 min) isotropic resolution were also performed at 7 T with a new sequence encoding scheme.

Results: UTE imaging generated positive contrast and higher SNR and CNR whatever the magnetic field and the USPIO concentration used compared to pre-contrast images. Time-resolved 3D acquisition enables high blood SNR (66.6 ± 4.5 at 7 T) and CNR (33.2 ± 4.2 at 7 T) without flow or motion artefact. Coronary arteries and aortic valve were visible on images acquired at 104 μm resolution.

Conclusions: We have demonstrated that by combining the injection of iron nanoparticles with 3D-cine UTE sequences, it was possible to generate a strong positive contrast between blood and surrounding tissues. These properties were exploited to produce images of the cardiovascular system in small animals at high magnetic fields with a high spatial and temporal resolution. This approach might be useful to measure the functional cardiac parameters or to assess anatomical modifications to the blood vessels in cardio-vascular disease models.

No MeSH data available.


Related in: MedlinePlus