Limits...
Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis.

Zhao Q, Guo Z, Piao S, Wang C, An T - Proteome Sci (2015)

Bottom Line: In this study, we showed most of porcine oocytes became nuclear matured at 33 h of IVM and the rate had no significant difference with oocytes at 42 h of IVM (p > 0.05).Moreover, the cleavage and blastocyst rates of SCNT and PA embryos derived from 42O were significantly higher than that of 33O (p < 0.05).These results present a unique insight into maternal factors related to nuclear reprogramming and early embryo development.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Northeast Forestry University, 26 Hexing Road, Xiangfang Dist., Harbin, Helongjiang 150040 China.

ABSTRACT

Background: Differentiated cell nuclei can be reprogrammed to a pluripotent state in several ways, including incubation with oocyte extracts, transfer into enucleated oocytes, and induced pluripotent stem cell technology. Nuclear transfer-mediated reprogramming has been proven to be the most efficient method. Maternal factors stored in oocytes have critical roles on nuclear reprogramming and early embryo development, but remain elusive.

Results: In this study, we showed most of porcine oocytes became nuclear matured at 33 h of IVM and the rate had no significant difference with oocytes at 42 h of IVM (p > 0.05). Moreover, the cleavage and blastocyst rates of SCNT and PA embryos derived from 42O were significantly higher than that of 33O (p < 0.05). But 33O could sustain IVF embryo development with higher cleavage and blastocyst rates comparing to 42O (p < 0.05). To clarify the development potential difference between 33O and 42O, 18 differentially expressed proteins were identified by proteomic analysis, and randomly selected proteins were confirmed by Western blot. Bioinformatic analysis of these proteins revealed that 33O highly synthesized proteins related to fertilization, and 42O was rich in nuclear reprogramming factors.

Conclusions: These results present a unique insight into maternal factors related to nuclear reprogramming and early embryo development.

No MeSH data available.


The classification of the identified proteins was performed according to the gene ontology term “molecular function”, “biological process” and “cellular component”
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4493956&req=5

Fig5: The classification of the identified proteins was performed according to the gene ontology term “molecular function”, “biological process” and “cellular component”

Mentions: We categorized proteins identified in this study by searching Gene Ontology and performing a literature search. The differentially expressed proteins were classified into groups based on molecular function (structural constituent of cytoskeleton, unfolded protein binding, nucleotide binding, NAD or NADH binding, ribonucleotide binding, calcium ion binding and ATP binding), biological process (cellular homeostasis, response to inorganic substance, response to drug, intermediate, cell death, cellular ion homeostasis, maintenance of location, antioxidation and monosaccharide catabolic), and cellular component (cytoplasm, membrane, endoplasmic reticulum, cytoskeleton, cytosol, perinuclear region of cytoplasm and nucleus) with the UniProt database (Fig. 5). DAVID and Agilent Literature Search were combined by cytoscape to cluster most affected molecular functions in oocyte maturation, namely embryonic development, embryogenesis, ovulation, epigenetic modification, fertilization, meiosis, ageing, chromatin remodeling and anti-ageing. Additionally, we employed a detailed analysis of embryonic development, using automated text-mining tool which enables the software to generate pathways from entries in the PubMed database and Gene Ontology. These analysis revealed that proteins related to fertilization were found to be highly synthesized in 33O, such as PDIA3 [16], and nuclear reprogramming was significantly affected by high expressed proteins in 42O, such as PARK7 that was proved to be required for successful development of porcine SCNT embryos [22].Fig. 5


Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis.

Zhao Q, Guo Z, Piao S, Wang C, An T - Proteome Sci (2015)

The classification of the identified proteins was performed according to the gene ontology term “molecular function”, “biological process” and “cellular component”
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493956&req=5

Fig5: The classification of the identified proteins was performed according to the gene ontology term “molecular function”, “biological process” and “cellular component”
Mentions: We categorized proteins identified in this study by searching Gene Ontology and performing a literature search. The differentially expressed proteins were classified into groups based on molecular function (structural constituent of cytoskeleton, unfolded protein binding, nucleotide binding, NAD or NADH binding, ribonucleotide binding, calcium ion binding and ATP binding), biological process (cellular homeostasis, response to inorganic substance, response to drug, intermediate, cell death, cellular ion homeostasis, maintenance of location, antioxidation and monosaccharide catabolic), and cellular component (cytoplasm, membrane, endoplasmic reticulum, cytoskeleton, cytosol, perinuclear region of cytoplasm and nucleus) with the UniProt database (Fig. 5). DAVID and Agilent Literature Search were combined by cytoscape to cluster most affected molecular functions in oocyte maturation, namely embryonic development, embryogenesis, ovulation, epigenetic modification, fertilization, meiosis, ageing, chromatin remodeling and anti-ageing. Additionally, we employed a detailed analysis of embryonic development, using automated text-mining tool which enables the software to generate pathways from entries in the PubMed database and Gene Ontology. These analysis revealed that proteins related to fertilization were found to be highly synthesized in 33O, such as PDIA3 [16], and nuclear reprogramming was significantly affected by high expressed proteins in 42O, such as PARK7 that was proved to be required for successful development of porcine SCNT embryos [22].Fig. 5

Bottom Line: In this study, we showed most of porcine oocytes became nuclear matured at 33 h of IVM and the rate had no significant difference with oocytes at 42 h of IVM (p > 0.05).Moreover, the cleavage and blastocyst rates of SCNT and PA embryos derived from 42O were significantly higher than that of 33O (p < 0.05).These results present a unique insight into maternal factors related to nuclear reprogramming and early embryo development.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Northeast Forestry University, 26 Hexing Road, Xiangfang Dist., Harbin, Helongjiang 150040 China.

ABSTRACT

Background: Differentiated cell nuclei can be reprogrammed to a pluripotent state in several ways, including incubation with oocyte extracts, transfer into enucleated oocytes, and induced pluripotent stem cell technology. Nuclear transfer-mediated reprogramming has been proven to be the most efficient method. Maternal factors stored in oocytes have critical roles on nuclear reprogramming and early embryo development, but remain elusive.

Results: In this study, we showed most of porcine oocytes became nuclear matured at 33 h of IVM and the rate had no significant difference with oocytes at 42 h of IVM (p > 0.05). Moreover, the cleavage and blastocyst rates of SCNT and PA embryos derived from 42O were significantly higher than that of 33O (p < 0.05). But 33O could sustain IVF embryo development with higher cleavage and blastocyst rates comparing to 42O (p < 0.05). To clarify the development potential difference between 33O and 42O, 18 differentially expressed proteins were identified by proteomic analysis, and randomly selected proteins were confirmed by Western blot. Bioinformatic analysis of these proteins revealed that 33O highly synthesized proteins related to fertilization, and 42O was rich in nuclear reprogramming factors.

Conclusions: These results present a unique insight into maternal factors related to nuclear reprogramming and early embryo development.

No MeSH data available.