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Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity.

Zhao J, Li Q, Pan CL, Liu JC, Wang HY, Tan LS, Pan YP - BMC Microbiol. (2015)

Bottom Line: P.gingivalis W83 has a pathogenic effect on host oral cavity.Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile.These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, School of Stomatology, China Medical University, Shenyang , Liaoning, China. cmu.zhaojian@163.com.

ABSTRACT

Background: The development of chronic periodontitis was due to not only periodontal pathogens, but also the interaction between periodontal pathogens and host. The aim of this study is to investigate the alterations in gene expression in Porphyromonas gingivalis (P.gingivalis) W83 after inoculation in rat oral cavity.

Results: P.gingivalis W83 inoculation in rat oral cavity caused inflammatory responses in gingival tissues and destroyed host alveolar bone. Microarray analysis revealed that 42 genes were upregulated, and 22 genes were downregulated in the detected 1786 genes in the inoculated P.gingivalis W83. Real-time quantitative PCR detection confirmed the expression alterations in some selected genes. Products of these upregulated and downregulated genes are mainly related to transposon functions, cell transmembrane transportation, protein and nucleic acid metabolism, energy metabolism, cell division and bacterial pathogenicity.

Conclusions: P.gingivalis W83 has a pathogenic effect on host oral cavity. Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile. These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

No MeSH data available.


Related in: MedlinePlus

Isolation and identification of inoculated P.gingivalis W83. (A) Some suspicious black colonies were found from plaque mixture. (B) Suspious black colonies were pure cultured. (C) Gram staining proved the pure culture as gram-negative brevibacterium (×400). (D) Agarose gel electrophoresis proved that PCR fragment length was 857 bp. 1 and 2: Wild type P.gingivalis W83; 3–8: three inoculated P.gingivalis W83 samples for microarray analysis (two columns for each sample)
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Fig2: Isolation and identification of inoculated P.gingivalis W83. (A) Some suspicious black colonies were found from plaque mixture. (B) Suspious black colonies were pure cultured. (C) Gram staining proved the pure culture as gram-negative brevibacterium (×400). (D) Agarose gel electrophoresis proved that PCR fragment length was 857 bp. 1 and 2: Wild type P.gingivalis W83; 3–8: three inoculated P.gingivalis W83 samples for microarray analysis (two columns for each sample)

Mentions: Suspicious gram-negative bacilli were taken from plaque culture. After pure subculture, gram staining and polymerase chain reaction (PCR) proved that the bacteria were P.gingivalis W83. PCR fragment length of the product was 857 bp, as shown in Fig. 2.Fig. 2


Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity.

Zhao J, Li Q, Pan CL, Liu JC, Wang HY, Tan LS, Pan YP - BMC Microbiol. (2015)

Isolation and identification of inoculated P.gingivalis W83. (A) Some suspicious black colonies were found from plaque mixture. (B) Suspious black colonies were pure cultured. (C) Gram staining proved the pure culture as gram-negative brevibacterium (×400). (D) Agarose gel electrophoresis proved that PCR fragment length was 857 bp. 1 and 2: Wild type P.gingivalis W83; 3–8: three inoculated P.gingivalis W83 samples for microarray analysis (two columns for each sample)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493947&req=5

Fig2: Isolation and identification of inoculated P.gingivalis W83. (A) Some suspicious black colonies were found from plaque mixture. (B) Suspious black colonies were pure cultured. (C) Gram staining proved the pure culture as gram-negative brevibacterium (×400). (D) Agarose gel electrophoresis proved that PCR fragment length was 857 bp. 1 and 2: Wild type P.gingivalis W83; 3–8: three inoculated P.gingivalis W83 samples for microarray analysis (two columns for each sample)
Mentions: Suspicious gram-negative bacilli were taken from plaque culture. After pure subculture, gram staining and polymerase chain reaction (PCR) proved that the bacteria were P.gingivalis W83. PCR fragment length of the product was 857 bp, as shown in Fig. 2.Fig. 2

Bottom Line: P.gingivalis W83 has a pathogenic effect on host oral cavity.Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile.These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, School of Stomatology, China Medical University, Shenyang , Liaoning, China. cmu.zhaojian@163.com.

ABSTRACT

Background: The development of chronic periodontitis was due to not only periodontal pathogens, but also the interaction between periodontal pathogens and host. The aim of this study is to investigate the alterations in gene expression in Porphyromonas gingivalis (P.gingivalis) W83 after inoculation in rat oral cavity.

Results: P.gingivalis W83 inoculation in rat oral cavity caused inflammatory responses in gingival tissues and destroyed host alveolar bone. Microarray analysis revealed that 42 genes were upregulated, and 22 genes were downregulated in the detected 1786 genes in the inoculated P.gingivalis W83. Real-time quantitative PCR detection confirmed the expression alterations in some selected genes. Products of these upregulated and downregulated genes are mainly related to transposon functions, cell transmembrane transportation, protein and nucleic acid metabolism, energy metabolism, cell division and bacterial pathogenicity.

Conclusions: P.gingivalis W83 has a pathogenic effect on host oral cavity. Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile. These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

No MeSH data available.


Related in: MedlinePlus