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Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity.

Zhao J, Li Q, Pan CL, Liu JC, Wang HY, Tan LS, Pan YP - BMC Microbiol. (2015)

Bottom Line: P.gingivalis W83 has a pathogenic effect on host oral cavity.Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile.These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, School of Stomatology, China Medical University, Shenyang , Liaoning, China. cmu.zhaojian@163.com.

ABSTRACT

Background: The development of chronic periodontitis was due to not only periodontal pathogens, but also the interaction between periodontal pathogens and host. The aim of this study is to investigate the alterations in gene expression in Porphyromonas gingivalis (P.gingivalis) W83 after inoculation in rat oral cavity.

Results: P.gingivalis W83 inoculation in rat oral cavity caused inflammatory responses in gingival tissues and destroyed host alveolar bone. Microarray analysis revealed that 42 genes were upregulated, and 22 genes were downregulated in the detected 1786 genes in the inoculated P.gingivalis W83. Real-time quantitative PCR detection confirmed the expression alterations in some selected genes. Products of these upregulated and downregulated genes are mainly related to transposon functions, cell transmembrane transportation, protein and nucleic acid metabolism, energy metabolism, cell division and bacterial pathogenicity.

Conclusions: P.gingivalis W83 has a pathogenic effect on host oral cavity. Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile. These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

No MeSH data available.


Related in: MedlinePlus

Pathogenic effects of P.gingivalis W83 on rat oral cavity. (A) The gingival tissues were inflammatory and bleeding after P.gingivalis W83 inoculation in rat oral cavity for 8 weeks. (B) Severe alveolar bone losses were found in rats with P.gingivalis W83 inoculation. Left: control groups. Right: rats inoculated with P.gingivalis W83 intraorally for 8 weeks
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Fig1: Pathogenic effects of P.gingivalis W83 on rat oral cavity. (A) The gingival tissues were inflammatory and bleeding after P.gingivalis W83 inoculation in rat oral cavity for 8 weeks. (B) Severe alveolar bone losses were found in rats with P.gingivalis W83 inoculation. Left: control groups. Right: rats inoculated with P.gingivalis W83 intraorally for 8 weeks

Mentions: After P.gingivalis W83 inoculation in rat oral cavity for 8 weeks, the gingival tissues were inflammatory and bleeding (Fig. 1A). Severe alveolar bone losses were found in rats with P.gingivalis W83 inoculation (Fig. 1B). The distance between cementoenamel junction and alveolar bone crest (CEJ: ABC) was measured at proximal, middle and distal sites of buccal and palatal per molar, respectively. In first molars, second molars, third molars, the distances were significantly increased, which were 1216.00 ± 305.98 μm, 987.28 ± 238.14 μm, 725.11 ± 202.71 μm, compared with normal rats, which were 414.89 ± 209.67 μm, 300.44 ± 127.92 μm, 357.56 ± 281.06 μm.Fig. 1


Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity.

Zhao J, Li Q, Pan CL, Liu JC, Wang HY, Tan LS, Pan YP - BMC Microbiol. (2015)

Pathogenic effects of P.gingivalis W83 on rat oral cavity. (A) The gingival tissues were inflammatory and bleeding after P.gingivalis W83 inoculation in rat oral cavity for 8 weeks. (B) Severe alveolar bone losses were found in rats with P.gingivalis W83 inoculation. Left: control groups. Right: rats inoculated with P.gingivalis W83 intraorally for 8 weeks
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493947&req=5

Fig1: Pathogenic effects of P.gingivalis W83 on rat oral cavity. (A) The gingival tissues were inflammatory and bleeding after P.gingivalis W83 inoculation in rat oral cavity for 8 weeks. (B) Severe alveolar bone losses were found in rats with P.gingivalis W83 inoculation. Left: control groups. Right: rats inoculated with P.gingivalis W83 intraorally for 8 weeks
Mentions: After P.gingivalis W83 inoculation in rat oral cavity for 8 weeks, the gingival tissues were inflammatory and bleeding (Fig. 1A). Severe alveolar bone losses were found in rats with P.gingivalis W83 inoculation (Fig. 1B). The distance between cementoenamel junction and alveolar bone crest (CEJ: ABC) was measured at proximal, middle and distal sites of buccal and palatal per molar, respectively. In first molars, second molars, third molars, the distances were significantly increased, which were 1216.00 ± 305.98 μm, 987.28 ± 238.14 μm, 725.11 ± 202.71 μm, compared with normal rats, which were 414.89 ± 209.67 μm, 300.44 ± 127.92 μm, 357.56 ± 281.06 μm.Fig. 1

Bottom Line: P.gingivalis W83 has a pathogenic effect on host oral cavity.Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile.These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, School of Stomatology, China Medical University, Shenyang , Liaoning, China. cmu.zhaojian@163.com.

ABSTRACT

Background: The development of chronic periodontitis was due to not only periodontal pathogens, but also the interaction between periodontal pathogens and host. The aim of this study is to investigate the alterations in gene expression in Porphyromonas gingivalis (P.gingivalis) W83 after inoculation in rat oral cavity.

Results: P.gingivalis W83 inoculation in rat oral cavity caused inflammatory responses in gingival tissues and destroyed host alveolar bone. Microarray analysis revealed that 42 genes were upregulated, and 22 genes were downregulated in the detected 1786 genes in the inoculated P.gingivalis W83. Real-time quantitative PCR detection confirmed the expression alterations in some selected genes. Products of these upregulated and downregulated genes are mainly related to transposon functions, cell transmembrane transportation, protein and nucleic acid metabolism, energy metabolism, cell division and bacterial pathogenicity.

Conclusions: P.gingivalis W83 has a pathogenic effect on host oral cavity. Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile. These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

No MeSH data available.


Related in: MedlinePlus