Limits...
The Neuroprotective Effect of Tetramethylpyrazine Against Contusive Spinal Cord Injury by Activating PGC-1α in Rats.

Hu J, Lang Y, Cao Y, Zhang T, Lu H - Neurochem. Res. (2015)

Bottom Line: TMP treatment markedly increased PGC-1α expression, neuronal survival and BBB locomotor scores, while also reducing neural apoptosis.These results demonstrate that TMP is neuroprotective against contusive SCI, with the inhibition of neural apoptosis and increase of neuronal survival.The sustained expression of PGC-1α may partially contribute to the TMP-mediated neuroprotective effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, China.

ABSTRACT
Tetramethylpyrazine (TMP) has been suggested to have neuroprotective effects against spinal cord injury (SCI); however, few studies have examined these effects and the corresponding mechanism. Therefore, the present study aimed to investigate the neuroprotective effect and underlying mechanism of TMP against contusive SCI. Adult male Sprague-Dawley rats were randomly divided into Sham, normal saline (NS) and TMP groups. Each group was divided into subgroups according to the time of sacrifice: 1, 3, 7, 14, 21 and 28 days post-injury. Laminectomy was performed in all groups, followed by contusive SCI establishment in the TMP and NS groups. TMP (80 mg/kg) was injected thereafter daily from 3 to 7 days post-injury in the TMP group, which was replaced by equal volume of normal saline in the NS group. The Basso-Beattie-Bresnahan (BBB) Locomotor Rating Scale was measured at different time points post-injury to appraise locomotor functional recovery. Quantitative real-time PCR and immunofluorescence were used to assess the spatio-temporal expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), while western blot was adopted to detect the effect of TMP on PGC-1α. Neural apoptotic changes and neuronal survival were evaluated using the TUNEL method and Nissl staining, respectively. TMP treatment markedly increased PGC-1α expression, neuronal survival and BBB locomotor scores, while also reducing neural apoptosis. These results demonstrate that TMP is neuroprotective against contusive SCI, with the inhibition of neural apoptosis and increase of neuronal survival. The sustained expression of PGC-1α may partially contribute to the TMP-mediated neuroprotective effect.

No MeSH data available.


Related in: MedlinePlus

Effect of tetramethylpyrazine (TMP) treatment on neuronal survival. Representative Nissl staining (a) shows that at 7 days post-injury there were more surviving neurons in the TMP group than in the NS group, which were closer to the neurons in the Sham group. The mottled bluish violet stained typical morphological features of cells indicates neurons. Inside the black box are the examples of each group. Scale bar 100 μm. The quantitative data (b) demonstrates that the TMP group (n = 5) displayed a significantly increased number of neurons compared with that of the NS group (n = 5) at 7 days post-injury. **P < 0.01 comparison between the TMP and NS group
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4493940&req=5

Fig6: Effect of tetramethylpyrazine (TMP) treatment on neuronal survival. Representative Nissl staining (a) shows that at 7 days post-injury there were more surviving neurons in the TMP group than in the NS group, which were closer to the neurons in the Sham group. The mottled bluish violet stained typical morphological features of cells indicates neurons. Inside the black box are the examples of each group. Scale bar 100 μm. The quantitative data (b) demonstrates that the TMP group (n = 5) displayed a significantly increased number of neurons compared with that of the NS group (n = 5) at 7 days post-injury. **P < 0.01 comparison between the TMP and NS group

Mentions: Next, we detected whether there was any difference in neuronal survival at 7 days post-injury using Nissl staining. Our results demonstrate that there were markedly more Nissl-positive cells with good morphology in the TMP group compared with that of the NS group (Fig. 6). This showed that TMP treatment promoted the survival of neurons after contusive SCI.Fig. 6


The Neuroprotective Effect of Tetramethylpyrazine Against Contusive Spinal Cord Injury by Activating PGC-1α in Rats.

Hu J, Lang Y, Cao Y, Zhang T, Lu H - Neurochem. Res. (2015)

Effect of tetramethylpyrazine (TMP) treatment on neuronal survival. Representative Nissl staining (a) shows that at 7 days post-injury there were more surviving neurons in the TMP group than in the NS group, which were closer to the neurons in the Sham group. The mottled bluish violet stained typical morphological features of cells indicates neurons. Inside the black box are the examples of each group. Scale bar 100 μm. The quantitative data (b) demonstrates that the TMP group (n = 5) displayed a significantly increased number of neurons compared with that of the NS group (n = 5) at 7 days post-injury. **P < 0.01 comparison between the TMP and NS group
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4493940&req=5

Fig6: Effect of tetramethylpyrazine (TMP) treatment on neuronal survival. Representative Nissl staining (a) shows that at 7 days post-injury there were more surviving neurons in the TMP group than in the NS group, which were closer to the neurons in the Sham group. The mottled bluish violet stained typical morphological features of cells indicates neurons. Inside the black box are the examples of each group. Scale bar 100 μm. The quantitative data (b) demonstrates that the TMP group (n = 5) displayed a significantly increased number of neurons compared with that of the NS group (n = 5) at 7 days post-injury. **P < 0.01 comparison between the TMP and NS group
Mentions: Next, we detected whether there was any difference in neuronal survival at 7 days post-injury using Nissl staining. Our results demonstrate that there were markedly more Nissl-positive cells with good morphology in the TMP group compared with that of the NS group (Fig. 6). This showed that TMP treatment promoted the survival of neurons after contusive SCI.Fig. 6

Bottom Line: TMP treatment markedly increased PGC-1α expression, neuronal survival and BBB locomotor scores, while also reducing neural apoptosis.These results demonstrate that TMP is neuroprotective against contusive SCI, with the inhibition of neural apoptosis and increase of neuronal survival.The sustained expression of PGC-1α may partially contribute to the TMP-mediated neuroprotective effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, China.

ABSTRACT
Tetramethylpyrazine (TMP) has been suggested to have neuroprotective effects against spinal cord injury (SCI); however, few studies have examined these effects and the corresponding mechanism. Therefore, the present study aimed to investigate the neuroprotective effect and underlying mechanism of TMP against contusive SCI. Adult male Sprague-Dawley rats were randomly divided into Sham, normal saline (NS) and TMP groups. Each group was divided into subgroups according to the time of sacrifice: 1, 3, 7, 14, 21 and 28 days post-injury. Laminectomy was performed in all groups, followed by contusive SCI establishment in the TMP and NS groups. TMP (80 mg/kg) was injected thereafter daily from 3 to 7 days post-injury in the TMP group, which was replaced by equal volume of normal saline in the NS group. The Basso-Beattie-Bresnahan (BBB) Locomotor Rating Scale was measured at different time points post-injury to appraise locomotor functional recovery. Quantitative real-time PCR and immunofluorescence were used to assess the spatio-temporal expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), while western blot was adopted to detect the effect of TMP on PGC-1α. Neural apoptotic changes and neuronal survival were evaluated using the TUNEL method and Nissl staining, respectively. TMP treatment markedly increased PGC-1α expression, neuronal survival and BBB locomotor scores, while also reducing neural apoptosis. These results demonstrate that TMP is neuroprotective against contusive SCI, with the inhibition of neural apoptosis and increase of neuronal survival. The sustained expression of PGC-1α may partially contribute to the TMP-mediated neuroprotective effect.

No MeSH data available.


Related in: MedlinePlus