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Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.


Thein vitrocytotoxicity assay against human liver BEL-7402 cells (48 h);p < 0.05.
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Fig7: Thein vitrocytotoxicity assay against human liver BEL-7402 cells (48 h);p < 0.05.

Mentions: The cytotoxicity of the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs was investigated and compared with bulk HCPT using BEL-7402 cells. As shown in Figure 7, at equivalent concentrations of HCPT, the HCPT-loaded PEG-b-PLA NPs showed higher cytotoxicity than the HCPT-loaded PLA NPs at equivalent concentrations of HCPT. The possible reasons are as follows. Firstly, the release rate of the HCPT-loaded PEG-b-PLA NPs was faster, and the total HCPT released was much more than HCPT-loaded PLA NPs (see Figure 6). Secondly, the particle size of the HCPT-loaded PEG-b-PLA NPs was much smaller, leading to the easier cellular uptake and more drug accumulation inside the cell and thus to the enhanced cytotoxicity. HCPT showed the highest inhibition rate, mainly ascribing to the fact that HCPT would directly act on the target site inside the cells without drug release with increasing intracellular drug concentration to a high level within 24 h.Figure 7


Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

Thein vitrocytotoxicity assay against human liver BEL-7402 cells (48 h);p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493845&req=5

Fig7: Thein vitrocytotoxicity assay against human liver BEL-7402 cells (48 h);p < 0.05.
Mentions: The cytotoxicity of the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs was investigated and compared with bulk HCPT using BEL-7402 cells. As shown in Figure 7, at equivalent concentrations of HCPT, the HCPT-loaded PEG-b-PLA NPs showed higher cytotoxicity than the HCPT-loaded PLA NPs at equivalent concentrations of HCPT. The possible reasons are as follows. Firstly, the release rate of the HCPT-loaded PEG-b-PLA NPs was faster, and the total HCPT released was much more than HCPT-loaded PLA NPs (see Figure 6). Secondly, the particle size of the HCPT-loaded PEG-b-PLA NPs was much smaller, leading to the easier cellular uptake and more drug accumulation inside the cell and thus to the enhanced cytotoxicity. HCPT showed the highest inhibition rate, mainly ascribing to the fact that HCPT would directly act on the target site inside the cells without drug release with increasing intracellular drug concentration to a high level within 24 h.Figure 7

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.