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Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.


CLSM images of HCPT-loaded PEG-b-PLA NPs (A) and HCPT-loaded PLA NPs (B).
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Fig5: CLSM images of HCPT-loaded PEG-b-PLA NPs (A) and HCPT-loaded PLA NPs (B).

Mentions: The distribution of HCPT was showed in Figure 5. The green fluorescence imaging (excitation at 382 nm) was performed to visualize HCPT in the NPs. In the HCPT-loaded PLA NPs, green fluorescence imaging overlapped nicely with the image of the particles, which indicated that HCPT was uniformly distributed within the particles. However, in the HCPT-loaded PEG-b-PLA NPs, the green fluorescence only appeared in the core of the NPs, demonstrating that HCPT only existed in the hydrophobic core of the particles. The results of the TEM and CLSM images both confirm the core-shell architectures of the HCPT-loaded PEG-b-PLA NPs: HCPT and PLA formed the core, and PEG formed the shell. With the hydrophilic shell, the steric stabilization of the NPs was enhanced in the dispersion, and the particle size was decreased.Figure 5


Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

CLSM images of HCPT-loaded PEG-b-PLA NPs (A) and HCPT-loaded PLA NPs (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493845&req=5

Fig5: CLSM images of HCPT-loaded PEG-b-PLA NPs (A) and HCPT-loaded PLA NPs (B).
Mentions: The distribution of HCPT was showed in Figure 5. The green fluorescence imaging (excitation at 382 nm) was performed to visualize HCPT in the NPs. In the HCPT-loaded PLA NPs, green fluorescence imaging overlapped nicely with the image of the particles, which indicated that HCPT was uniformly distributed within the particles. However, in the HCPT-loaded PEG-b-PLA NPs, the green fluorescence only appeared in the core of the NPs, demonstrating that HCPT only existed in the hydrophobic core of the particles. The results of the TEM and CLSM images both confirm the core-shell architectures of the HCPT-loaded PEG-b-PLA NPs: HCPT and PLA formed the core, and PEG formed the shell. With the hydrophilic shell, the steric stabilization of the NPs was enhanced in the dispersion, and the particle size was decreased.Figure 5

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.