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Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.


Electron microscopy images. SEM images of HCPT-loaded PLA NPs (A) and HCPT-loaded PEG-b-PLA NPs (B) and TEM images of HCPT-loaded PLA NPs (C) and HCPT-loaded PEG-b-PLA NPs (D).
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Fig4: Electron microscopy images. SEM images of HCPT-loaded PLA NPs (A) and HCPT-loaded PEG-b-PLA NPs (B) and TEM images of HCPT-loaded PLA NPs (C) and HCPT-loaded PEG-b-PLA NPs (D).

Mentions: The SEM images revealed that the HCPT-loaded PEG-b-PLA NPs were regularly spherical in shape and had a relatively smooth surface with a little more than 100 nm in particle size, which was in accordance with the result of DLS. While the HCPT-loaded PLA NPs showed uniform color in TEM images, the HCPT-loaded PEG-b-PLA NPs contained a core particle with a lighter outer region (Figure 4), which might represent the hydrophobic core, made up of PLA and HCPT, and the hydrophilic shell, made up of PEG chain, respectively.Figure 4


Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

Electron microscopy images. SEM images of HCPT-loaded PLA NPs (A) and HCPT-loaded PEG-b-PLA NPs (B) and TEM images of HCPT-loaded PLA NPs (C) and HCPT-loaded PEG-b-PLA NPs (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493845&req=5

Fig4: Electron microscopy images. SEM images of HCPT-loaded PLA NPs (A) and HCPT-loaded PEG-b-PLA NPs (B) and TEM images of HCPT-loaded PLA NPs (C) and HCPT-loaded PEG-b-PLA NPs (D).
Mentions: The SEM images revealed that the HCPT-loaded PEG-b-PLA NPs were regularly spherical in shape and had a relatively smooth surface with a little more than 100 nm in particle size, which was in accordance with the result of DLS. While the HCPT-loaded PLA NPs showed uniform color in TEM images, the HCPT-loaded PEG-b-PLA NPs contained a core particle with a lighter outer region (Figure 4), which might represent the hydrophobic core, made up of PLA and HCPT, and the hydrophilic shell, made up of PEG chain, respectively.Figure 4

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.