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Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.


Particle size and zeta potential. Particle size and zeta potential of HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs.
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Fig3: Particle size and zeta potential. Particle size and zeta potential of HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs.

Mentions: Figure 3 showed that the particle size of the HCPT-loaded PEG-b-PLA NPs was 120.1 ± 4.9 nm, much smaller than that of the HCPT-loaded PLA NPs (226.8 ± 10.4 nm), indicating that the HCPT-loaded PEG-b-PLA NPs will be taken up easier by cancer cells, for smaller particle size favors EPR targeting (enhanced permeability and retention effect). The reason may be that the hydrophilicity of the polymer becomes stronger with PEGylation, which can attain lower energy state than that without PEGylation and form smaller particles. Moreover, due to its lower PDI (0.057), the HCPT-loaded PEG-b-PLA NPs would have a better dispersibility and stability than the HCPT-loaded PLA NPs. Figure 3 also showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a zeta potential of −31.2 and −45.7 mV, for the oxygen atom of the PEG chains could combine with a few hydrogen ions, which was positively charged, and made the zeta potential lower. However, the zeta potential of the two NPs was high enough to support that these NPs could not aggregate much in aqueous state in general and in physiologically relevant media in particular.Figure 3


Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

Particle size and zeta potential. Particle size and zeta potential of HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493845&req=5

Fig3: Particle size and zeta potential. Particle size and zeta potential of HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs.
Mentions: Figure 3 showed that the particle size of the HCPT-loaded PEG-b-PLA NPs was 120.1 ± 4.9 nm, much smaller than that of the HCPT-loaded PLA NPs (226.8 ± 10.4 nm), indicating that the HCPT-loaded PEG-b-PLA NPs will be taken up easier by cancer cells, for smaller particle size favors EPR targeting (enhanced permeability and retention effect). The reason may be that the hydrophilicity of the polymer becomes stronger with PEGylation, which can attain lower energy state than that without PEGylation and form smaller particles. Moreover, due to its lower PDI (0.057), the HCPT-loaded PEG-b-PLA NPs would have a better dispersibility and stability than the HCPT-loaded PLA NPs. Figure 3 also showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a zeta potential of −31.2 and −45.7 mV, for the oxygen atom of the PEG chains could combine with a few hydrogen ions, which was positively charged, and made the zeta potential lower. However, the zeta potential of the two NPs was high enough to support that these NPs could not aggregate much in aqueous state in general and in physiologically relevant media in particular.Figure 3

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.