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Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.


Schematic representations of HCPT-loaded PLA NPs and HCPT-loaded PEG-b-PLA NPs.
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Fig1: Schematic representations of HCPT-loaded PLA NPs and HCPT-loaded PEG-b-PLA NPs.

Mentions: HCPT has a rather poor solubility for most organic solvents, so NaOH solution is used to dissolve HCPT. Acetone is water-miscible and a good solvent for PEG-b-PLA, so it is chosen as the organic phase. Firstly, PEG-b-PLA was dissolved in acetone (solution A) and HCPT was dissolved in NaOH solution (solution B). When solution B was dropped into solution A, PEG-b-PLA and HCPT were codissolved in this mixed solution, which was used as the organic phase and then extensively dialyzed against the aqueous phase. In the dialysis process, acetone was gradually removed and slowly replaced with water. To attain the minimal energy state, the hydrophobic PLA assembled together and formed a core while the hydrophilic PEG extended to the aqueous environment to form a shell (Figure 1). Because of the driving force of hydrophobic interaction, HCPT spontaneously transferred into the hydrophobic cores of the NPs. Although both of PEG-PLA and PLA particles were prepared by the same dialysis method, their formation mechanisms are different: the amphiphilic PEG-PLA formed micelles-like particles with core-shell structure; however, the homopolymer of PLA formed homogeneous solid particles. The determined drug entrapment efficiency and drug-loaded content of HCPT-loaded PEG-b-PLA NPs determined by ultraviolet spectrophotometry were 44.52% ± 0.42% and 7.42% ± 0.07%, respectively, and those of HCPT-loaded PLA NPs were 44.94% ± 0.54% and 7.49% ± 0.09%, respectively.Figure 1


Evaluation of self-assembled HCPT-loaded PEG-b-PLA nanoparticles by comparing with HCPT-loaded PLA nanoparticles.

Yang X, Wu S, Wang Y, Li Y, Chang D, Luo Y, Ye S, Hou Z - Nanoscale Res Lett (2014)

Schematic representations of HCPT-loaded PLA NPs and HCPT-loaded PEG-b-PLA NPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493845&req=5

Fig1: Schematic representations of HCPT-loaded PLA NPs and HCPT-loaded PEG-b-PLA NPs.
Mentions: HCPT has a rather poor solubility for most organic solvents, so NaOH solution is used to dissolve HCPT. Acetone is water-miscible and a good solvent for PEG-b-PLA, so it is chosen as the organic phase. Firstly, PEG-b-PLA was dissolved in acetone (solution A) and HCPT was dissolved in NaOH solution (solution B). When solution B was dropped into solution A, PEG-b-PLA and HCPT were codissolved in this mixed solution, which was used as the organic phase and then extensively dialyzed against the aqueous phase. In the dialysis process, acetone was gradually removed and slowly replaced with water. To attain the minimal energy state, the hydrophobic PLA assembled together and formed a core while the hydrophilic PEG extended to the aqueous environment to form a shell (Figure 1). Because of the driving force of hydrophobic interaction, HCPT spontaneously transferred into the hydrophobic cores of the NPs. Although both of PEG-PLA and PLA particles were prepared by the same dialysis method, their formation mechanisms are different: the amphiphilic PEG-PLA formed micelles-like particles with core-shell structure; however, the homopolymer of PLA formed homogeneous solid particles. The determined drug entrapment efficiency and drug-loaded content of HCPT-loaded PEG-b-PLA NPs determined by ultraviolet spectrophotometry were 44.52% ± 0.42% and 7.42% ± 0.07%, respectively, and those of HCPT-loaded PLA NPs were 44.94% ± 0.54% and 7.49% ± 0.09%, respectively.Figure 1

Bottom Line: The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively.The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs.These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China, yangxiangruix@126.com.

ABSTRACT
We present a dialysis technique to prepare the 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (PEG-b-PLA) and PLA, respectively. Both HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs were characterized by differential scanning calorimetry (DSC), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The results showed that the HCPT-loaded PEG-b-PLA NPs and HCPT-loaded PLA NPs presented a hydrodynamic particle size of 120.1 and 226.8 nm, with a polydispersity index of 0.057 and 0.207, a zeta potential of -31.2 and -45.7 mV, drug encapsulation efficiency of 44.52% and 44.94%, and drug-loaded content of 7.42% and 7.49%, respectively. The HCPT-loaded PEG-b-PLA NPs presented faster drug release rate compared to the HCPT-loaded PLA NPs. The HCPT-loaded PEG-b-PLA NPs presented higher cytotoxicity than the HCPT-loaded PLA NPs. These results suggested that the HCPT-loaded PEG-b-PLA NPs presented better characteristics for drug delivery compared to HCPT-loaded PLA NPs.

No MeSH data available.