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iTRAQ protein profile analysis of neuroblastoma (NA) cells infected with the rabies viruses rHep-Flury and Hep-dG.

Yang Y, Liu W, Yan G, Luo Y, Zhao J, Yang X, Mei M, Wu X, Guo X - Front Microbiol (2015)

Bottom Line: Bioinformatics analysis of the distinct protein suggested that glycoprotein over-expression in the attenuated RABV strain can induce activation of the interferon signaling.Furthermore, it may promote the antiviral response, MHC-I mediated antigen-specific T cell immune response, apoptosis and autophagy in an IFN-dependent manner.These findings might not only improve the understanding of the dynamics of RABV and host interaction, but also help understand the mechanisms underlying innate and adaptive immunity during RABV infection.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University Guangzhou, China.

ABSTRACT
The rabies virus (RABV) glycoprotein (G) is the principal contributor to the pathogenicity and protective immunity of RABV. In a previous work, we reported that recombinant rabies virus Hep-dG, which was generated by reverse genetics to carry two copies of the G-gene, showed lower virulence than the parental virus rHep-Flury in suckling mice with a better immune protection effect. To better understand the mechanisms underlying rabies virus attenuation and the role of glycoprotein G, isobaric tags for relative and absolute quantitation (iTRAQ) was performed to identify and quantify distinct proteins. 10 and 111 differentially expressed proteins were obtained in rHep-Flury and Hep-dG infection groups, respectively. Selected data were validated by western blot and qRT-PCR. Bioinformatics analysis of the distinct protein suggested that glycoprotein over-expression in the attenuated RABV strain can induce activation of the interferon signaling. Furthermore, it may promote the antiviral response, MHC-I mediated antigen-specific T cell immune response, apoptosis and autophagy in an IFN-dependent manner. These findings might not only improve the understanding of the dynamics of RABV and host interaction, but also help understand the mechanisms underlying innate and adaptive immunity during RABV infection.

No MeSH data available.


Related in: MedlinePlus

Functional characterizations of differentially expressed proteins in rabies virus Hep-dG infection group. (A) Top canonical pathways. (B) Diseases and disorders. (C) Molecular and cellular functions. (D) Physiological system development and functions. (E) Clinical chemistry and hematology. (F) Cardiotoxicity. (G) Hepatotoxicity. (H) Nephrotoxicity.
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Figure 4: Functional characterizations of differentially expressed proteins in rabies virus Hep-dG infection group. (A) Top canonical pathways. (B) Diseases and disorders. (C) Molecular and cellular functions. (D) Physiological system development and functions. (E) Clinical chemistry and hematology. (F) Cardiotoxicity. (G) Hepatotoxicity. (H) Nephrotoxicity.

Mentions: Canonical pathways analysis can predict the significantly regulated pathways base on the submitted information of altered proteins. Top canonical pathways in Hep-dG-infected cells comprised activation of IRF by cytosolic pattern recognition receptors (5 out of a possible 64 molecules, P = 6.31 × 10−6), acute phase response signaling (6 out of a possible 169 molecules, P = 6.68 × 10−5), role of RIG1-like receptors in antiviral innate immunity (3 out of a possible 45 molecules, P = 8.21 × 10−4), ERK/MAPK signaling (5 out of a possible 187 molecules, P = 1.01 × 10−3), and TNFR1 signaling (3 out of a possible 49 molecules, P = 1.05 × 10−3) (Figure 4A).


iTRAQ protein profile analysis of neuroblastoma (NA) cells infected with the rabies viruses rHep-Flury and Hep-dG.

Yang Y, Liu W, Yan G, Luo Y, Zhao J, Yang X, Mei M, Wu X, Guo X - Front Microbiol (2015)

Functional characterizations of differentially expressed proteins in rabies virus Hep-dG infection group. (A) Top canonical pathways. (B) Diseases and disorders. (C) Molecular and cellular functions. (D) Physiological system development and functions. (E) Clinical chemistry and hematology. (F) Cardiotoxicity. (G) Hepatotoxicity. (H) Nephrotoxicity.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4493837&req=5

Figure 4: Functional characterizations of differentially expressed proteins in rabies virus Hep-dG infection group. (A) Top canonical pathways. (B) Diseases and disorders. (C) Molecular and cellular functions. (D) Physiological system development and functions. (E) Clinical chemistry and hematology. (F) Cardiotoxicity. (G) Hepatotoxicity. (H) Nephrotoxicity.
Mentions: Canonical pathways analysis can predict the significantly regulated pathways base on the submitted information of altered proteins. Top canonical pathways in Hep-dG-infected cells comprised activation of IRF by cytosolic pattern recognition receptors (5 out of a possible 64 molecules, P = 6.31 × 10−6), acute phase response signaling (6 out of a possible 169 molecules, P = 6.68 × 10−5), role of RIG1-like receptors in antiviral innate immunity (3 out of a possible 45 molecules, P = 8.21 × 10−4), ERK/MAPK signaling (5 out of a possible 187 molecules, P = 1.01 × 10−3), and TNFR1 signaling (3 out of a possible 49 molecules, P = 1.05 × 10−3) (Figure 4A).

Bottom Line: Bioinformatics analysis of the distinct protein suggested that glycoprotein over-expression in the attenuated RABV strain can induce activation of the interferon signaling.Furthermore, it may promote the antiviral response, MHC-I mediated antigen-specific T cell immune response, apoptosis and autophagy in an IFN-dependent manner.These findings might not only improve the understanding of the dynamics of RABV and host interaction, but also help understand the mechanisms underlying innate and adaptive immunity during RABV infection.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University Guangzhou, China.

ABSTRACT
The rabies virus (RABV) glycoprotein (G) is the principal contributor to the pathogenicity and protective immunity of RABV. In a previous work, we reported that recombinant rabies virus Hep-dG, which was generated by reverse genetics to carry two copies of the G-gene, showed lower virulence than the parental virus rHep-Flury in suckling mice with a better immune protection effect. To better understand the mechanisms underlying rabies virus attenuation and the role of glycoprotein G, isobaric tags for relative and absolute quantitation (iTRAQ) was performed to identify and quantify distinct proteins. 10 and 111 differentially expressed proteins were obtained in rHep-Flury and Hep-dG infection groups, respectively. Selected data were validated by western blot and qRT-PCR. Bioinformatics analysis of the distinct protein suggested that glycoprotein over-expression in the attenuated RABV strain can induce activation of the interferon signaling. Furthermore, it may promote the antiviral response, MHC-I mediated antigen-specific T cell immune response, apoptosis and autophagy in an IFN-dependent manner. These findings might not only improve the understanding of the dynamics of RABV and host interaction, but also help understand the mechanisms underlying innate and adaptive immunity during RABV infection.

No MeSH data available.


Related in: MedlinePlus