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iTRAQ protein profile analysis of neuroblastoma (NA) cells infected with the rabies viruses rHep-Flury and Hep-dG.

Yang Y, Liu W, Yan G, Luo Y, Zhao J, Yang X, Mei M, Wu X, Guo X - Front Microbiol (2015)

Bottom Line: Bioinformatics analysis of the distinct protein suggested that glycoprotein over-expression in the attenuated RABV strain can induce activation of the interferon signaling.Furthermore, it may promote the antiviral response, MHC-I mediated antigen-specific T cell immune response, apoptosis and autophagy in an IFN-dependent manner.These findings might not only improve the understanding of the dynamics of RABV and host interaction, but also help understand the mechanisms underlying innate and adaptive immunity during RABV infection.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University Guangzhou, China.

ABSTRACT
The rabies virus (RABV) glycoprotein (G) is the principal contributor to the pathogenicity and protective immunity of RABV. In a previous work, we reported that recombinant rabies virus Hep-dG, which was generated by reverse genetics to carry two copies of the G-gene, showed lower virulence than the parental virus rHep-Flury in suckling mice with a better immune protection effect. To better understand the mechanisms underlying rabies virus attenuation and the role of glycoprotein G, isobaric tags for relative and absolute quantitation (iTRAQ) was performed to identify and quantify distinct proteins. 10 and 111 differentially expressed proteins were obtained in rHep-Flury and Hep-dG infection groups, respectively. Selected data were validated by western blot and qRT-PCR. Bioinformatics analysis of the distinct protein suggested that glycoprotein over-expression in the attenuated RABV strain can induce activation of the interferon signaling. Furthermore, it may promote the antiviral response, MHC-I mediated antigen-specific T cell immune response, apoptosis and autophagy in an IFN-dependent manner. These findings might not only improve the understanding of the dynamics of RABV and host interaction, but also help understand the mechanisms underlying innate and adaptive immunity during RABV infection.

No MeSH data available.


Related in: MedlinePlus

An overview of differentially expressed proteins in rabies viruses rHep-Flury and Hep-dG infection group. (A) Among the significantly altered proteins, 10 exhibited a differential expression pattern in rHep-Flury-infected cells compared to mock-infected cells. Of these, 4 proteins were highly down-regulated and 6 significantly up-regulated. Meanwhile, 111 proteins were differentially expressed in Hep-dG-infected cells compared to mock- infected cells. Of these, 32 proteins were highly down-regulated and 79 significantly up-regulated. (B) Venn diagrams depict the overlap of distinct proteins identification between rHep-Flury and Hep-dG-infected group. The number of proteins that have higher (↑) or lower (↓) fold-change values in each comparison are also shown.
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Figure 3: An overview of differentially expressed proteins in rabies viruses rHep-Flury and Hep-dG infection group. (A) Among the significantly altered proteins, 10 exhibited a differential expression pattern in rHep-Flury-infected cells compared to mock-infected cells. Of these, 4 proteins were highly down-regulated and 6 significantly up-regulated. Meanwhile, 111 proteins were differentially expressed in Hep-dG-infected cells compared to mock- infected cells. Of these, 32 proteins were highly down-regulated and 79 significantly up-regulated. (B) Venn diagrams depict the overlap of distinct proteins identification between rHep-Flury and Hep-dG-infected group. The number of proteins that have higher (↑) or lower (↓) fold-change values in each comparison are also shown.

Mentions: Among the significantly altered proteins, 10 exhibited a differential expression pattern in rHep-Flury-infected cells compared to mock-infected cells (Supporting Information Table S1), of which 4 down-regulated and 6 up-regulated (Figure 3A). Meanwhile, 111 proteins were differentially expressed in Hep-dG-infected cells compared to mock- infected cells (Supporting Information Table S2), 32 being down-regulated and 79 up-regulated (Figure 3A). As shown in the Venn diagram, eight/113 differentially expressed proteins were common in both infection groups (Figure 3B).


iTRAQ protein profile analysis of neuroblastoma (NA) cells infected with the rabies viruses rHep-Flury and Hep-dG.

Yang Y, Liu W, Yan G, Luo Y, Zhao J, Yang X, Mei M, Wu X, Guo X - Front Microbiol (2015)

An overview of differentially expressed proteins in rabies viruses rHep-Flury and Hep-dG infection group. (A) Among the significantly altered proteins, 10 exhibited a differential expression pattern in rHep-Flury-infected cells compared to mock-infected cells. Of these, 4 proteins were highly down-regulated and 6 significantly up-regulated. Meanwhile, 111 proteins were differentially expressed in Hep-dG-infected cells compared to mock- infected cells. Of these, 32 proteins were highly down-regulated and 79 significantly up-regulated. (B) Venn diagrams depict the overlap of distinct proteins identification between rHep-Flury and Hep-dG-infected group. The number of proteins that have higher (↑) or lower (↓) fold-change values in each comparison are also shown.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4493837&req=5

Figure 3: An overview of differentially expressed proteins in rabies viruses rHep-Flury and Hep-dG infection group. (A) Among the significantly altered proteins, 10 exhibited a differential expression pattern in rHep-Flury-infected cells compared to mock-infected cells. Of these, 4 proteins were highly down-regulated and 6 significantly up-regulated. Meanwhile, 111 proteins were differentially expressed in Hep-dG-infected cells compared to mock- infected cells. Of these, 32 proteins were highly down-regulated and 79 significantly up-regulated. (B) Venn diagrams depict the overlap of distinct proteins identification between rHep-Flury and Hep-dG-infected group. The number of proteins that have higher (↑) or lower (↓) fold-change values in each comparison are also shown.
Mentions: Among the significantly altered proteins, 10 exhibited a differential expression pattern in rHep-Flury-infected cells compared to mock-infected cells (Supporting Information Table S1), of which 4 down-regulated and 6 up-regulated (Figure 3A). Meanwhile, 111 proteins were differentially expressed in Hep-dG-infected cells compared to mock- infected cells (Supporting Information Table S2), 32 being down-regulated and 79 up-regulated (Figure 3A). As shown in the Venn diagram, eight/113 differentially expressed proteins were common in both infection groups (Figure 3B).

Bottom Line: Bioinformatics analysis of the distinct protein suggested that glycoprotein over-expression in the attenuated RABV strain can induce activation of the interferon signaling.Furthermore, it may promote the antiviral response, MHC-I mediated antigen-specific T cell immune response, apoptosis and autophagy in an IFN-dependent manner.These findings might not only improve the understanding of the dynamics of RABV and host interaction, but also help understand the mechanisms underlying innate and adaptive immunity during RABV infection.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University Guangzhou, China.

ABSTRACT
The rabies virus (RABV) glycoprotein (G) is the principal contributor to the pathogenicity and protective immunity of RABV. In a previous work, we reported that recombinant rabies virus Hep-dG, which was generated by reverse genetics to carry two copies of the G-gene, showed lower virulence than the parental virus rHep-Flury in suckling mice with a better immune protection effect. To better understand the mechanisms underlying rabies virus attenuation and the role of glycoprotein G, isobaric tags for relative and absolute quantitation (iTRAQ) was performed to identify and quantify distinct proteins. 10 and 111 differentially expressed proteins were obtained in rHep-Flury and Hep-dG infection groups, respectively. Selected data were validated by western blot and qRT-PCR. Bioinformatics analysis of the distinct protein suggested that glycoprotein over-expression in the attenuated RABV strain can induce activation of the interferon signaling. Furthermore, it may promote the antiviral response, MHC-I mediated antigen-specific T cell immune response, apoptosis and autophagy in an IFN-dependent manner. These findings might not only improve the understanding of the dynamics of RABV and host interaction, but also help understand the mechanisms underlying innate and adaptive immunity during RABV infection.

No MeSH data available.


Related in: MedlinePlus