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Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds.

Kim HJ, Silva JE, Vu HS, Mockaitis K, Nam JW, Cahoon EB - J. Exp. Bot. (2015)

Bottom Line: Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0.Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs.Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

No MeSH data available.


Related in: MedlinePlus

Fatty acid composition (weight%) of seed lipids in CsKASII-RNAi Camelina expressing UcFatB1. Fatty acids were extracted from transgenic Camelina seeds and analysed using gas chromatography. Values are the means ±SD of five biological replicates.
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Figure 7: Fatty acid composition (weight%) of seed lipids in CsKASII-RNAi Camelina expressing UcFatB1. Fatty acids were extracted from transgenic Camelina seeds and analysed using gas chromatography. Values are the means ±SD of five biological replicates.

Mentions: To determine whether KASII suppression increases MCFA levels, the KASII-RNAi construct was transformed into a Camelina line homozygous for the UcFatB1 transgene. The resulting 12:0 content in seeds from these lines ranged from 3 mol% to 7 mol% versus 15 mol% (or 5% versus 10% on a weight percent basis to total oil) in the parent line lacking KASII-RNAi suppression (Table 4; Fig. 7). The mol% of C8–C16 fatty acids in KASII-RNAi lines was not significantly affected by suppression in either the wild type or the UcFatB1 background (Table 4), and ranged from 24.5% to 33.7% of total fatty acid in both backgrounds. These results suggested that efficient downstream acylation of MCFAs into TAG is important for the stable accumulation of MCFAs in the transgenic Camelina seeds. In this regard, with high levels of 16:0 production, it is likely that 16:0-CoA is used in preference to 12:0-CoA for acylation on the glycerol backbone.


Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds.

Kim HJ, Silva JE, Vu HS, Mockaitis K, Nam JW, Cahoon EB - J. Exp. Bot. (2015)

Fatty acid composition (weight%) of seed lipids in CsKASII-RNAi Camelina expressing UcFatB1. Fatty acids were extracted from transgenic Camelina seeds and analysed using gas chromatography. Values are the means ±SD of five biological replicates.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493788&req=5

Figure 7: Fatty acid composition (weight%) of seed lipids in CsKASII-RNAi Camelina expressing UcFatB1. Fatty acids were extracted from transgenic Camelina seeds and analysed using gas chromatography. Values are the means ±SD of five biological replicates.
Mentions: To determine whether KASII suppression increases MCFA levels, the KASII-RNAi construct was transformed into a Camelina line homozygous for the UcFatB1 transgene. The resulting 12:0 content in seeds from these lines ranged from 3 mol% to 7 mol% versus 15 mol% (or 5% versus 10% on a weight percent basis to total oil) in the parent line lacking KASII-RNAi suppression (Table 4; Fig. 7). The mol% of C8–C16 fatty acids in KASII-RNAi lines was not significantly affected by suppression in either the wild type or the UcFatB1 background (Table 4), and ranged from 24.5% to 33.7% of total fatty acid in both backgrounds. These results suggested that efficient downstream acylation of MCFAs into TAG is important for the stable accumulation of MCFAs in the transgenic Camelina seeds. In this regard, with high levels of 16:0 production, it is likely that 16:0-CoA is used in preference to 12:0-CoA for acylation on the glycerol backbone.

Bottom Line: Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0.Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs.Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

No MeSH data available.


Related in: MedlinePlus