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Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds.

Kim HJ, Silva JE, Vu HS, Mockaitis K, Nam JW, Cahoon EB - J. Exp. Bot. (2015)

Bottom Line: Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0.Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs.Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

No MeSH data available.


Related in: MedlinePlus

Total fatty acid contents of engineered Camelina lines. Total fatty acid contents of seeds from FatB with or without CnLPAT expression were analysed by gas chromatography. The data represents means ±SD with five biological replicates. Asterisks indicate statistical differences compared with the wild type (*P<0.05).
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Figure 6: Total fatty acid contents of engineered Camelina lines. Total fatty acid contents of seeds from FatB with or without CnLPAT expression were analysed by gas chromatography. The data represents means ±SD with five biological replicates. Asterisks indicate statistical differences compared with the wild type (*P<0.05).

Mentions: Similar to a previous report by Tjellström et al. (2013), a positive correlation between 8:0 and 10:0 levels and 18:1 levels was observed. Seeds expressing CpuFatB3, for example, had a 2-fold increase of 18:1. However, although 12:0, 14:0, and 16:0 increase in seeds expressing UcFatB1, CpFatB2, and CpuFatB1, the level of 18:1 decreased to 8, 6, and 7 mol%, respectively, compared with wild-type seeds that had 11 mol% of 18:1 (Table 2). Furthermore, total fatty acid content in 14:0-accumulating seeds expressing CpFatB2 was increased, whereas a decrease in total fatty acids was detected in seeds of 10:0-producing FatBs, in comparison with wild-type seeds (Supplementary Fig. S1 at JXB online). Notably, co-expression of MCFA-specific FatB and CnLPAT resulted in an increase of MCFA without significant impact on total seed fatty acid content under greenhouse growth conditions (Fig. 6). Interestingly, lines expressing the C10-producing ChFatB2 thioesterase had lower seed fatty acid content than wild-type controls, but this was restored to wild-type levels with co-expression of CnLPAT (Fig. 6).


Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds.

Kim HJ, Silva JE, Vu HS, Mockaitis K, Nam JW, Cahoon EB - J. Exp. Bot. (2015)

Total fatty acid contents of engineered Camelina lines. Total fatty acid contents of seeds from FatB with or without CnLPAT expression were analysed by gas chromatography. The data represents means ±SD with five biological replicates. Asterisks indicate statistical differences compared with the wild type (*P<0.05).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493788&req=5

Figure 6: Total fatty acid contents of engineered Camelina lines. Total fatty acid contents of seeds from FatB with or without CnLPAT expression were analysed by gas chromatography. The data represents means ±SD with five biological replicates. Asterisks indicate statistical differences compared with the wild type (*P<0.05).
Mentions: Similar to a previous report by Tjellström et al. (2013), a positive correlation between 8:0 and 10:0 levels and 18:1 levels was observed. Seeds expressing CpuFatB3, for example, had a 2-fold increase of 18:1. However, although 12:0, 14:0, and 16:0 increase in seeds expressing UcFatB1, CpFatB2, and CpuFatB1, the level of 18:1 decreased to 8, 6, and 7 mol%, respectively, compared with wild-type seeds that had 11 mol% of 18:1 (Table 2). Furthermore, total fatty acid content in 14:0-accumulating seeds expressing CpFatB2 was increased, whereas a decrease in total fatty acids was detected in seeds of 10:0-producing FatBs, in comparison with wild-type seeds (Supplementary Fig. S1 at JXB online). Notably, co-expression of MCFA-specific FatB and CnLPAT resulted in an increase of MCFA without significant impact on total seed fatty acid content under greenhouse growth conditions (Fig. 6). Interestingly, lines expressing the C10-producing ChFatB2 thioesterase had lower seed fatty acid content than wild-type controls, but this was restored to wild-type levels with co-expression of CnLPAT (Fig. 6).

Bottom Line: Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0.Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs.Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

No MeSH data available.


Related in: MedlinePlus