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Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds.

Kim HJ, Silva JE, Vu HS, Mockaitis K, Nam JW, Cahoon EB - J. Exp. Bot. (2015)

Bottom Line: Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0.Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs.Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

No MeSH data available.


Related in: MedlinePlus

Spatial expression of FatB acyl-ACP thioesterases in C. pulcherrima and C. viscosissima. Total RNA was isolated from individual tissues and converted into cDNAs for RT-PCR analyses for evaluation of FatB gene expression in different tissues of C. pulcherrima and C.viscossisima. Cuphea eIF4-a1 and actin genes were used as an internal control for RT-PCR.
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Figure 2: Spatial expression of FatB acyl-ACP thioesterases in C. pulcherrima and C. viscosissima. Total RNA was isolated from individual tissues and converted into cDNAs for RT-PCR analyses for evaluation of FatB gene expression in different tissues of C. pulcherrima and C.viscossisima. Cuphea eIF4-a1 and actin genes were used as an internal control for RT-PCR.

Mentions: Real-time PCR (RT-PCR) analysis was carried out with total RNAs isolated from roots, stems, leaves, flowers, and developing seeds of C. pulcherrima and C. viscosissima to examine relative expression levels of FatB transcripts from the 454 transcriptomic analyses. Among the four FatB genes from C. pulcherrima, CpuFatB1 and CpuFatB2 displayed ubiquitous expression in the tested organs, whereas CpuFatB4 was expressed predominantly in developing seeds, and CpuFatB3 was expressed exclusively in developing seeds (Fig. 2A). Among the three FatB genes in C. viscosissima, expression of CvFatB1 and CvFatB3 was detected only in developing seeds, while expression of CvFatB2 was detected in all tested organs (Fig. 2B).


Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds.

Kim HJ, Silva JE, Vu HS, Mockaitis K, Nam JW, Cahoon EB - J. Exp. Bot. (2015)

Spatial expression of FatB acyl-ACP thioesterases in C. pulcherrima and C. viscosissima. Total RNA was isolated from individual tissues and converted into cDNAs for RT-PCR analyses for evaluation of FatB gene expression in different tissues of C. pulcherrima and C.viscossisima. Cuphea eIF4-a1 and actin genes were used as an internal control for RT-PCR.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493788&req=5

Figure 2: Spatial expression of FatB acyl-ACP thioesterases in C. pulcherrima and C. viscosissima. Total RNA was isolated from individual tissues and converted into cDNAs for RT-PCR analyses for evaluation of FatB gene expression in different tissues of C. pulcherrima and C.viscossisima. Cuphea eIF4-a1 and actin genes were used as an internal control for RT-PCR.
Mentions: Real-time PCR (RT-PCR) analysis was carried out with total RNAs isolated from roots, stems, leaves, flowers, and developing seeds of C. pulcherrima and C. viscosissima to examine relative expression levels of FatB transcripts from the 454 transcriptomic analyses. Among the four FatB genes from C. pulcherrima, CpuFatB1 and CpuFatB2 displayed ubiquitous expression in the tested organs, whereas CpuFatB4 was expressed predominantly in developing seeds, and CpuFatB3 was expressed exclusively in developing seeds (Fig. 2A). Among the three FatB genes in C. viscosissima, expression of CvFatB1 and CvFatB3 was detected only in developing seeds, while expression of CvFatB2 was detected in all tested organs (Fig. 2B).

Bottom Line: Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0.Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs.Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

No MeSH data available.


Related in: MedlinePlus