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Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds.

Kim HJ, Silva JE, Vu HS, Mockaitis K, Nam JW, Cahoon EB - J. Exp. Bot. (2015)

Bottom Line: Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0.Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs.Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

No MeSH data available.


Related in: MedlinePlus

Fatty acid composition of seeds and leaves in Cuphea pulcherrima and Cuphea viscosissima. Fatty acids were extracted from leaves and seeds of C. pulcherrima and C. viscosissima and analysed using gas chromatography. Values are the means ±SD from five biological replicates.
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Figure 1: Fatty acid composition of seeds and leaves in Cuphea pulcherrima and Cuphea viscosissima. Fatty acids were extracted from leaves and seeds of C. pulcherrima and C. viscosissima and analysed using gas chromatography. Values are the means ±SD from five biological replicates.

Mentions: Seeds of C. pulcherrima and C. viscosissima accumulate 94 mol% of 8:0, and 17 mol% of 8:0 and 70 mol% of 10:0 fatty acids, respectively (Graham and Kleiman, 1992; Phippen et al., 2006). To confirm the fatty acid profile of Cuphea seeds and to compare their profile with other tissues, the fatty acid composition was analysed in mature dried seeds and leaves. Seeds and leaves of C. pulcherrima and C. viscosissima had very different fatty acid compositions (Fig. 1). As previously reported, 8:0 accounted for nearly 95 mol% of fatty acids in C. pulcherrima seeds, while the leaves contained primarily 16:0 and large amounts of unsaturated long fatty acids, including 18:3, which made up 61 mol% of the total fatty acids. Similar results were observed in C. viscosissima (Fig. 1). Nearly 90 mol% of the fatty acids in C. viscosissima seed consisted of 8:0 and 10:0, but leaves were enriched in 18:3 in amounts similar to those in C. pulcherrima leaves. This result confirmed that seeds of C. pulcherrima and C. viscosissima are excellent genetic resources for caprylic acid (8:0) and capric acid (10:0).


Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds.

Kim HJ, Silva JE, Vu HS, Mockaitis K, Nam JW, Cahoon EB - J. Exp. Bot. (2015)

Fatty acid composition of seeds and leaves in Cuphea pulcherrima and Cuphea viscosissima. Fatty acids were extracted from leaves and seeds of C. pulcherrima and C. viscosissima and analysed using gas chromatography. Values are the means ±SD from five biological replicates.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493788&req=5

Figure 1: Fatty acid composition of seeds and leaves in Cuphea pulcherrima and Cuphea viscosissima. Fatty acids were extracted from leaves and seeds of C. pulcherrima and C. viscosissima and analysed using gas chromatography. Values are the means ±SD from five biological replicates.
Mentions: Seeds of C. pulcherrima and C. viscosissima accumulate 94 mol% of 8:0, and 17 mol% of 8:0 and 70 mol% of 10:0 fatty acids, respectively (Graham and Kleiman, 1992; Phippen et al., 2006). To confirm the fatty acid profile of Cuphea seeds and to compare their profile with other tissues, the fatty acid composition was analysed in mature dried seeds and leaves. Seeds and leaves of C. pulcherrima and C. viscosissima had very different fatty acid compositions (Fig. 1). As previously reported, 8:0 accounted for nearly 95 mol% of fatty acids in C. pulcherrima seeds, while the leaves contained primarily 16:0 and large amounts of unsaturated long fatty acids, including 18:3, which made up 61 mol% of the total fatty acids. Similar results were observed in C. viscosissima (Fig. 1). Nearly 90 mol% of the fatty acids in C. viscosissima seed consisted of 8:0 and 10:0, but leaves were enriched in 18:3 in amounts similar to those in C. pulcherrima leaves. This result confirmed that seeds of C. pulcherrima and C. viscosissima are excellent genetic resources for caprylic acid (8:0) and capric acid (10:0).

Bottom Line: Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0.Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs.Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

No MeSH data available.


Related in: MedlinePlus