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How cell wall complexity influences saccharification efficiency in Miscanthus sinensis.

De Souza AP, Alvim Kamei CL, Torres AF, Pattathil S, Hahn MG, Trindade LM, Buckeridge MS - J. Exp. Bot. (2015)

Bottom Line: However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure.Cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures.When saccharification efficiency was correlated negatively with lignin, the structural features of arabinoxylan and xyloglucan were found to contribute positively to hydrolysis.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Physiological Ecology (LAFIECO), Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão 277, Sao Paulo, SP, Brazil.

No MeSH data available.


Related in: MedlinePlus

Principal component analysis (PCA) of the cell wall variables that showed statistically significant differences according to GLM results (P<0.05). (A) Data from the three genotypes showing the saccharification vector separating mainly the H0198 genotype from the others at PC1. (B) Data from H0120 and H0116 genotypes (both inversely correlated with lignin content) showing the saccharification vector at PC1. P-values indicate statistically significant differences of PCs. (This figure is available in colour at JXB online.)
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Figure 7: Principal component analysis (PCA) of the cell wall variables that showed statistically significant differences according to GLM results (P<0.05). (A) Data from the three genotypes showing the saccharification vector separating mainly the H0198 genotype from the others at PC1. (B) Data from H0120 and H0116 genotypes (both inversely correlated with lignin content) showing the saccharification vector at PC1. P-values indicate statistically significant differences of PCs. (This figure is available in colour at JXB online.)

Mentions: In order to check this hypothesis, a PCA considering all cell wall components found to be statistically significant among genotypes was performed. The PCA revealed clear differences among the three genotypes, with PC1 contributing with 47% of the total variation (Fig. 7A). PC1 distinguished H0198 from the other two genotypes. At the same time, this principal component also displays the highest contribution for saccharification efficiency (Supplementary Table S3 at JXB online). This makes PC1 the most adequate component to distinguish features of cell wall polysaccharides correlated to saccharification or recalcitrance. Therefore, the percentages contributions to saccharification or recalcitrance (Table 2) were calculated from PC1 loadings (Supplementary Table S3). The variables chosen as the highest influences on either feature were the ones in the top 50% of the PC1. Among these selected variables, the 25 favouring saccharification efficiency were monoclonal antibodies detecting epitopes in polysaccharides belonging to the classes of pectins (~28%), mannans (16%), β-glucan (~4%), and xylan-5 (2%) (Table 2A). These variables are associated mainly with the 0.1M NaOH- and sodium chlorite-solubilized cell wall fractions. Twenty variables were shown to be associated with higher recalcitrance, corresponding to different variables and epitopes, including: xyloglucan (~26%), xylans (~13%), pectins (~8%), and galactose in the 4M NaOH extract (2.8%), and the percentage of hemicelluloses (2.5%) (Table 2B). The xylans and xyloglucans related to recalcitrance are associated with the ammonium oxalate- and 4M NaOH-solubilized wall fractions. Additionally, some pectins present in the ammonium oxalate extract and the residue seem to be related to recalcitrance. Although xylans and pectins are also present in the group of variables that favours saccharification efficiency, the cell wall fractions from which these polysaccharides were released were completely different (e.g. pectins released from sodium chlorite, and 0.1M and 1M NaOH cell wall extracts favour saccharification efficiency, while pectins from ammonium oxalate-solubilized and residue wall fractions favour recalcitrance). Moreover, it is possible to observe differences in antibody epitopes related to saccharification efficiency and recalcitrance.


How cell wall complexity influences saccharification efficiency in Miscanthus sinensis.

De Souza AP, Alvim Kamei CL, Torres AF, Pattathil S, Hahn MG, Trindade LM, Buckeridge MS - J. Exp. Bot. (2015)

Principal component analysis (PCA) of the cell wall variables that showed statistically significant differences according to GLM results (P<0.05). (A) Data from the three genotypes showing the saccharification vector separating mainly the H0198 genotype from the others at PC1. (B) Data from H0120 and H0116 genotypes (both inversely correlated with lignin content) showing the saccharification vector at PC1. P-values indicate statistically significant differences of PCs. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493786&req=5

Figure 7: Principal component analysis (PCA) of the cell wall variables that showed statistically significant differences according to GLM results (P<0.05). (A) Data from the three genotypes showing the saccharification vector separating mainly the H0198 genotype from the others at PC1. (B) Data from H0120 and H0116 genotypes (both inversely correlated with lignin content) showing the saccharification vector at PC1. P-values indicate statistically significant differences of PCs. (This figure is available in colour at JXB online.)
Mentions: In order to check this hypothesis, a PCA considering all cell wall components found to be statistically significant among genotypes was performed. The PCA revealed clear differences among the three genotypes, with PC1 contributing with 47% of the total variation (Fig. 7A). PC1 distinguished H0198 from the other two genotypes. At the same time, this principal component also displays the highest contribution for saccharification efficiency (Supplementary Table S3 at JXB online). This makes PC1 the most adequate component to distinguish features of cell wall polysaccharides correlated to saccharification or recalcitrance. Therefore, the percentages contributions to saccharification or recalcitrance (Table 2) were calculated from PC1 loadings (Supplementary Table S3). The variables chosen as the highest influences on either feature were the ones in the top 50% of the PC1. Among these selected variables, the 25 favouring saccharification efficiency were monoclonal antibodies detecting epitopes in polysaccharides belonging to the classes of pectins (~28%), mannans (16%), β-glucan (~4%), and xylan-5 (2%) (Table 2A). These variables are associated mainly with the 0.1M NaOH- and sodium chlorite-solubilized cell wall fractions. Twenty variables were shown to be associated with higher recalcitrance, corresponding to different variables and epitopes, including: xyloglucan (~26%), xylans (~13%), pectins (~8%), and galactose in the 4M NaOH extract (2.8%), and the percentage of hemicelluloses (2.5%) (Table 2B). The xylans and xyloglucans related to recalcitrance are associated with the ammonium oxalate- and 4M NaOH-solubilized wall fractions. Additionally, some pectins present in the ammonium oxalate extract and the residue seem to be related to recalcitrance. Although xylans and pectins are also present in the group of variables that favours saccharification efficiency, the cell wall fractions from which these polysaccharides were released were completely different (e.g. pectins released from sodium chlorite, and 0.1M and 1M NaOH cell wall extracts favour saccharification efficiency, while pectins from ammonium oxalate-solubilized and residue wall fractions favour recalcitrance). Moreover, it is possible to observe differences in antibody epitopes related to saccharification efficiency and recalcitrance.

Bottom Line: However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure.Cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures.When saccharification efficiency was correlated negatively with lignin, the structural features of arabinoxylan and xyloglucan were found to contribute positively to hydrolysis.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Physiological Ecology (LAFIECO), Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão 277, Sao Paulo, SP, Brazil.

No MeSH data available.


Related in: MedlinePlus