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How cell wall complexity influences saccharification efficiency in Miscanthus sinensis.

De Souza AP, Alvim Kamei CL, Torres AF, Pattathil S, Hahn MG, Trindade LM, Buckeridge MS - J. Exp. Bot. (2015)

Bottom Line: However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure.Cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures.When saccharification efficiency was correlated negatively with lignin, the structural features of arabinoxylan and xyloglucan were found to contribute positively to hydrolysis.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Physiological Ecology (LAFIECO), Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão 277, Sao Paulo, SP, Brazil.

No MeSH data available.


Related in: MedlinePlus

Glycome profiles of each cell wall extract of three different genotypes (H0120, H0116, and H0198) of Miscanthus sinensis. On the right, the groups of antibodies used are shown, identified according to the polysaccharides predominantly recognized by each group. Coloured boxes indicate the main statistically significant differences among genotypes (n=5). See Supplementary Table S2 at JXB online for additional details about the individual antibodies used and statistics.
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Figure 4: Glycome profiles of each cell wall extract of three different genotypes (H0120, H0116, and H0198) of Miscanthus sinensis. On the right, the groups of antibodies used are shown, identified according to the polysaccharides predominantly recognized by each group. Coloured boxes indicate the main statistically significant differences among genotypes (n=5). See Supplementary Table S2 at JXB online for additional details about the individual antibodies used and statistics.

Mentions: The glycome profiles of cell wall extracts showed significant differences in the pattern and extractability of diverse glycan epitopes present in these three genotypes (Fig. 4; Supplementary Table S2 at JXB online). Specifically, the overall profiles of H0120 and H0116 are quite similar, and clearly distinct from the overall profile for H0198. For example, epitopes recognized by the xylan-5 clade of antibodies are absent in the sodium chlorite extract of the H0120 and H0116 genotypes, but present in H0198 (Fig. 4, yellow boxes). In contrast, the H0120 and H0116 genotypes appear to lack any mannan or β-glucan epitopes, while both sets of epitopes are clearly abundant in extracts of the H0198 genotype (Fig. 4, white boxes). The H0120 and H0116 genotypes had significantly higher levels of xyloglucan epitopes released in the oxalate extract than did the H0198 genotype (Fig. 4, orange boxes). H0116 appeared to contain higher levels of xyloglucan epitopes overall than did the other two genotypes (Fig. 4, green box). A few arabinogalactan-directed antibodies showed higher binding to wall extracts of H0198 [two antibodies in the middle of the rhamnogalacturonan I (RG-I)/arabinogalactan (AG) group and one in the AG-2 group] than were observed in extracts from the other two genotypes (Fig. 4, red boxes).


How cell wall complexity influences saccharification efficiency in Miscanthus sinensis.

De Souza AP, Alvim Kamei CL, Torres AF, Pattathil S, Hahn MG, Trindade LM, Buckeridge MS - J. Exp. Bot. (2015)

Glycome profiles of each cell wall extract of three different genotypes (H0120, H0116, and H0198) of Miscanthus sinensis. On the right, the groups of antibodies used are shown, identified according to the polysaccharides predominantly recognized by each group. Coloured boxes indicate the main statistically significant differences among genotypes (n=5). See Supplementary Table S2 at JXB online for additional details about the individual antibodies used and statistics.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493786&req=5

Figure 4: Glycome profiles of each cell wall extract of three different genotypes (H0120, H0116, and H0198) of Miscanthus sinensis. On the right, the groups of antibodies used are shown, identified according to the polysaccharides predominantly recognized by each group. Coloured boxes indicate the main statistically significant differences among genotypes (n=5). See Supplementary Table S2 at JXB online for additional details about the individual antibodies used and statistics.
Mentions: The glycome profiles of cell wall extracts showed significant differences in the pattern and extractability of diverse glycan epitopes present in these three genotypes (Fig. 4; Supplementary Table S2 at JXB online). Specifically, the overall profiles of H0120 and H0116 are quite similar, and clearly distinct from the overall profile for H0198. For example, epitopes recognized by the xylan-5 clade of antibodies are absent in the sodium chlorite extract of the H0120 and H0116 genotypes, but present in H0198 (Fig. 4, yellow boxes). In contrast, the H0120 and H0116 genotypes appear to lack any mannan or β-glucan epitopes, while both sets of epitopes are clearly abundant in extracts of the H0198 genotype (Fig. 4, white boxes). The H0120 and H0116 genotypes had significantly higher levels of xyloglucan epitopes released in the oxalate extract than did the H0198 genotype (Fig. 4, orange boxes). H0116 appeared to contain higher levels of xyloglucan epitopes overall than did the other two genotypes (Fig. 4, green box). A few arabinogalactan-directed antibodies showed higher binding to wall extracts of H0198 [two antibodies in the middle of the rhamnogalacturonan I (RG-I)/arabinogalactan (AG) group and one in the AG-2 group] than were observed in extracts from the other two genotypes (Fig. 4, red boxes).

Bottom Line: However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure.Cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures.When saccharification efficiency was correlated negatively with lignin, the structural features of arabinoxylan and xyloglucan were found to contribute positively to hydrolysis.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Physiological Ecology (LAFIECO), Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão 277, Sao Paulo, SP, Brazil.

No MeSH data available.


Related in: MedlinePlus