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How cell wall complexity influences saccharification efficiency in Miscanthus sinensis.

De Souza AP, Alvim Kamei CL, Torres AF, Pattathil S, Hahn MG, Trindade LM, Buckeridge MS - J. Exp. Bot. (2015)

Bottom Line: However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure.Cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures.When saccharification efficiency was correlated negatively with lignin, the structural features of arabinoxylan and xyloglucan were found to contribute positively to hydrolysis.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Physiological Ecology (LAFIECO), Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão 277, Sao Paulo, SP, Brazil.

No MeSH data available.


Related in: MedlinePlus

Oligosaccharide profiles obtained using lichenase (for detection of β-glucan) of cell wall fractions of stems of three different genotypes (H0120, H0116, and H0198) of Miscanthus sinensis. (a) Trisaccharides, (b) tetrasaccharides, and (c) pentasaccharide. Missing chromatograms from the ammonium oxalate extract of the H0116 and H0198 genotypes are due to the low amount of sample in this extract. Data on chromatograms are the average of three biological replicates.
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Figure 3: Oligosaccharide profiles obtained using lichenase (for detection of β-glucan) of cell wall fractions of stems of three different genotypes (H0120, H0116, and H0198) of Miscanthus sinensis. (a) Trisaccharides, (b) tetrasaccharides, and (c) pentasaccharide. Missing chromatograms from the ammonium oxalate extract of the H0116 and H0198 genotypes are due to the low amount of sample in this extract. Data on chromatograms are the average of three biological replicates.

Mentions: The oligosaccharides profiles obtained after digestion with lichenase showed that the mixed linkage β-glucan was extracted by sodium chlorite and sodium hydroxide (0.1, 1, and 4M) during the preparation of cell wallextracts of all three genotypes, with the exception of the 4M NaOH extract from H0120, in which no peaks associated with tri- or tetrasaccharides were observed (Fig. 3). No evidence for the presence of β-glucan in the residue (cellulose) remaining after the last base extraction was found by means of the oligosaccharide analyses. However, evidence that some β-glucan might be present in the residue was obtained in the glycome profiles (see below).


How cell wall complexity influences saccharification efficiency in Miscanthus sinensis.

De Souza AP, Alvim Kamei CL, Torres AF, Pattathil S, Hahn MG, Trindade LM, Buckeridge MS - J. Exp. Bot. (2015)

Oligosaccharide profiles obtained using lichenase (for detection of β-glucan) of cell wall fractions of stems of three different genotypes (H0120, H0116, and H0198) of Miscanthus sinensis. (a) Trisaccharides, (b) tetrasaccharides, and (c) pentasaccharide. Missing chromatograms from the ammonium oxalate extract of the H0116 and H0198 genotypes are due to the low amount of sample in this extract. Data on chromatograms are the average of three biological replicates.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493786&req=5

Figure 3: Oligosaccharide profiles obtained using lichenase (for detection of β-glucan) of cell wall fractions of stems of three different genotypes (H0120, H0116, and H0198) of Miscanthus sinensis. (a) Trisaccharides, (b) tetrasaccharides, and (c) pentasaccharide. Missing chromatograms from the ammonium oxalate extract of the H0116 and H0198 genotypes are due to the low amount of sample in this extract. Data on chromatograms are the average of three biological replicates.
Mentions: The oligosaccharides profiles obtained after digestion with lichenase showed that the mixed linkage β-glucan was extracted by sodium chlorite and sodium hydroxide (0.1, 1, and 4M) during the preparation of cell wallextracts of all three genotypes, with the exception of the 4M NaOH extract from H0120, in which no peaks associated with tri- or tetrasaccharides were observed (Fig. 3). No evidence for the presence of β-glucan in the residue (cellulose) remaining after the last base extraction was found by means of the oligosaccharide analyses. However, evidence that some β-glucan might be present in the residue was obtained in the glycome profiles (see below).

Bottom Line: However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure.Cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures.When saccharification efficiency was correlated negatively with lignin, the structural features of arabinoxylan and xyloglucan were found to contribute positively to hydrolysis.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Plant Physiological Ecology (LAFIECO), Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão 277, Sao Paulo, SP, Brazil.

No MeSH data available.


Related in: MedlinePlus