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Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation.

Maloney VJ, Park JY, Unda F, Mansfield SD - J. Exp. Bot. (2015)

Bottom Line: Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins.The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch.Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar.

View Article: PubMed Central - PubMed

Affiliation: Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.

No MeSH data available.


Growth comparison of Atsps–Arabidopsis plants expressing AtSPS and AtSPP simultaneously indicates their ability to outperform the Atsps– mutant, as well as the wild-type plants. (A) Height measurements of 2-month-old SPSR:SPPY and RSPS:SPPYArabidopsis plants compared with the Atsps– mutant plant. Error bars indicate the standard deviation. The line denotes significance at P=0.05. (B) Transgenic Arabidopsis seedlings. (C) Complementation of the Atsps– mutant line with SPSR–SPPY.
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Figure 8: Growth comparison of Atsps–Arabidopsis plants expressing AtSPS and AtSPP simultaneously indicates their ability to outperform the Atsps– mutant, as well as the wild-type plants. (A) Height measurements of 2-month-old SPSR:SPPY and RSPS:SPPYArabidopsis plants compared with the Atsps– mutant plant. Error bars indicate the standard deviation. The line denotes significance at P=0.05. (B) Transgenic Arabidopsis seedlings. (C) Complementation of the Atsps– mutant line with SPSR–SPPY.

Mentions: Increasing the activity of AtSPS and AtSPP affected plant growth rates in both Arabidopsis and hybrid poplar. In general, the height of the Arabidopsis cisgenic lines expressing AtSPS and AtSPP under the regulation of the 35S promoter in the Atsps– mutant background outperformed the wild type, as well as the Atsps– mutant lines, grown under similar conditions (Fig. 8). Arabidopsis plants transformed with two fused BRET constructs had >50% higher growth than the Atsps– mutant plants or the wild type (Fig. 8A). Moreover, the lines that were shown to have significant increases in the BRET ratio (>0.03) displayed substantially faster growth rates than the control (wild-type or Atsps–) plants (Fig. 8C). In hybrid poplar, the expression of the 35S::AtSPS-SPP fusion construct significantly increased both height and stem diameter in four of the five transgenic lines studied (Fig. 9). In contrast, expression of either AtSPP (data not shown) or AtSPS (Park et al., 2009) on their own in hybrid poplar did not manifest in increase in growth. In addition to the increased height and diameter growth, the wood density of the xylem was increased in all transgenic lines, as determined by X-ray densitometry (Table 1). Effectively, the combined growth increase with the enhanced cell wall density significantly increases the total biomass of the transgenic poplar trees over the wild-type controls growing concurrently.


Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation.

Maloney VJ, Park JY, Unda F, Mansfield SD - J. Exp. Bot. (2015)

Growth comparison of Atsps–Arabidopsis plants expressing AtSPS and AtSPP simultaneously indicates their ability to outperform the Atsps– mutant, as well as the wild-type plants. (A) Height measurements of 2-month-old SPSR:SPPY and RSPS:SPPYArabidopsis plants compared with the Atsps– mutant plant. Error bars indicate the standard deviation. The line denotes significance at P=0.05. (B) Transgenic Arabidopsis seedlings. (C) Complementation of the Atsps– mutant line with SPSR–SPPY.
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Related In: Results  -  Collection

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Figure 8: Growth comparison of Atsps–Arabidopsis plants expressing AtSPS and AtSPP simultaneously indicates their ability to outperform the Atsps– mutant, as well as the wild-type plants. (A) Height measurements of 2-month-old SPSR:SPPY and RSPS:SPPYArabidopsis plants compared with the Atsps– mutant plant. Error bars indicate the standard deviation. The line denotes significance at P=0.05. (B) Transgenic Arabidopsis seedlings. (C) Complementation of the Atsps– mutant line with SPSR–SPPY.
Mentions: Increasing the activity of AtSPS and AtSPP affected plant growth rates in both Arabidopsis and hybrid poplar. In general, the height of the Arabidopsis cisgenic lines expressing AtSPS and AtSPP under the regulation of the 35S promoter in the Atsps– mutant background outperformed the wild type, as well as the Atsps– mutant lines, grown under similar conditions (Fig. 8). Arabidopsis plants transformed with two fused BRET constructs had >50% higher growth than the Atsps– mutant plants or the wild type (Fig. 8A). Moreover, the lines that were shown to have significant increases in the BRET ratio (>0.03) displayed substantially faster growth rates than the control (wild-type or Atsps–) plants (Fig. 8C). In hybrid poplar, the expression of the 35S::AtSPS-SPP fusion construct significantly increased both height and stem diameter in four of the five transgenic lines studied (Fig. 9). In contrast, expression of either AtSPP (data not shown) or AtSPS (Park et al., 2009) on their own in hybrid poplar did not manifest in increase in growth. In addition to the increased height and diameter growth, the wood density of the xylem was increased in all transgenic lines, as determined by X-ray densitometry (Table 1). Effectively, the combined growth increase with the enhanced cell wall density significantly increases the total biomass of the transgenic poplar trees over the wild-type controls growing concurrently.

Bottom Line: Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins.The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch.Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar.

View Article: PubMed Central - PubMed

Affiliation: Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.

No MeSH data available.