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Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation.

Maloney VJ, Park JY, Unda F, Mansfield SD - J. Exp. Bot. (2015)

Bottom Line: Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins.The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch.Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar.

View Article: PubMed Central - PubMed

Affiliation: Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.

No MeSH data available.


YFP signal in AtSPP-SPS-YFP poplar plants indicates functionality of the 35SP::SPP–SPS–YFP vector.
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Figure 7: YFP signal in AtSPP-SPS-YFP poplar plants indicates functionality of the 35SP::SPP–SPS–YFP vector.

Mentions: In order to confirm the functionality of the 35SP::SPP-SPS-YFP vector, the presence of YFP fluorescence was confirmed using microscopy (Fig. 7). A strong YFP signal in all of the transgenic hybrid poplar lines clearly indicates that the foreign construct is being expressed and that the proteins are being produced.


Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation.

Maloney VJ, Park JY, Unda F, Mansfield SD - J. Exp. Bot. (2015)

YFP signal in AtSPP-SPS-YFP poplar plants indicates functionality of the 35SP::SPP–SPS–YFP vector.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493782&req=5

Figure 7: YFP signal in AtSPP-SPS-YFP poplar plants indicates functionality of the 35SP::SPP–SPS–YFP vector.
Mentions: In order to confirm the functionality of the 35SP::SPP-SPS-YFP vector, the presence of YFP fluorescence was confirmed using microscopy (Fig. 7). A strong YFP signal in all of the transgenic hybrid poplar lines clearly indicates that the foreign construct is being expressed and that the proteins are being produced.

Bottom Line: Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins.The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch.Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar.

View Article: PubMed Central - PubMed

Affiliation: Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.

No MeSH data available.